Semaphorin 3B (SEMA3B) (NM_004636) Human Tagged ORF Clone

CAT#: RC223532

SEMA3B (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B (SEMA3B), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417853) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223532 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SEMA3B
• Synonyms: LUCA-1; SemA; SEMA5; SEMAA; semaV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223532 representing NM_004636
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGGGCCGGGGCTGCCGCCGTGATCCCGGGCCTGGCCCTGCTCTGGGCAGTGGGGCTGGGGAGTG
  CCGCCCCCAGCCCCCCACGCCTTCGGCTCTCCTTCCAAGAGCTCCAGGCCTGGCATGGTCTCCAGACTTT
  CAGCCTGGAGCGAACCTGCTGCTACCAGGCCTTGCTGGTGGATGAGGAGCGTGGACGCCTGTTTGTGGGT
  GCCGAGAACCATGTGGCCTCCCTCAACCTGGACAACATCAGCAAGCGGGCCAAGAAGCTGGCCTGGCCGG
  CCCCTGTGGAATGGCGAGAGGAGTGCAACTGGGCAGGGAAGGACATTGGTACTGAGTGCATGAACTTCGT
  GAAGTTGCTGCATGCCTACAACCGCACCCATTTGCTGGCCTGTGGCACGGGAGCCTTCCACCCAACCTGT
  GCCTTTGTGGAAGTGGGCCACCGGGCAGAGGAGCCCGTCCTCCGGCTGGACCCAGGAAGGATAGAGGATG
  GCAAGGGGAAGAGTCCTTATGACCCCAGGCATCGGGCTGCCTCCGTGCTGGTGGGGGAGGAGCTATACTC
  AGGGGTGGCAGCAGACCTCATGGGACGAGACTTTACCATCTTTCGCAGCCTAGGGCAACGTCCAAGTCTC
  CGAACAGAGCCACACGACTCCCGCTGGCTCAATGAGCCCAAGTTTGTCAAGGTATTTTGGATCCCGGAGA
  GCGAGAACCCAGACGACGACAAAATCTACTTCTTCTTTCGTGAGACGGCGGTAGAGGCGGCGCCGGCACT
  GGGACGCCTGTCCGTGTCCCGCGTTGGCCAGATCTGCCGGAACGACGTGGGCGGCCAGCGCAGCCTGGTC
  AACAAGTGGACGACGTTCCTGAAGGCGCGGCTGGTGTGCTCGGTGCCCGGCGTCGAGGGCGACACCCACT
  TCGATCAGCTCCAGGATGTGTTTCTGTTGTCCTCGCGGGACCACCGGACCCCGCTGCTCTATGCCGTCTT
  CTCCACGTCCAGCAGCATCTTCCAGGGCTCTGCGGTGTGCGTGTACAGCATGAACGACGTGCGCCGGGCC
  TTCTTGGGACCCTTTGCACACAAGGAGGGGCCCATGCACCAGTGGGTGTCATACCAGGGTCGCGTCCCCT
  ACCCGCGGCCAGGCATGTGCCCCAGCAAGACCTTTGGCACCTTCAGTTCCACCAAGGACTTCCCAGACGA
  TGTCATCCAGTTTGCGCGGAACCACCCCCTCATGTACAACTCTGTCCTGCCCACTGGGGGGCGCCCTCTT
  TTCCTACAAGTTGGAGCCAATTACACCTTCACTCAAATTGCCGCGGACCGGGTTGCAGCCGCTGACGGAC
  ACTATGACGTCCTCTTCATTGGCACAGACGTTGGCACGGTGCTGAAGGTGATCTCGGTCCCCAAGGGCAG
  TAGGCCCAGCGCAGAGGGGCTGCTCCTGGAGGAGCTGCACGTGTTTGAGGACTCGGCCGCTGTCACCAGC
  ATGCAAATTTCTTCCAAGAGGCACCAGCTGTACGTAGCCTCGCGGAGCGCGGTGGCCCAGATCGCGTTGC
  ACCGCTGCGCTGCCCACGGCCGCGTCTGCACCGAATGCTGTCTGGCGCGTGACCCCTACTGCGCCTGGGA
  CGGGGTCGCGTGCACGCGCTTCCAGCCCAGTGCCAAGAGGCGGTTCCGGCGGCAAGACGTAAGGAATGGC
  GACCCCAGCACGTTGTGCTCCGGAGACTCGTCTCGTCCCGCGCTGCTGGAACACAAGGTGTTCGGCGTGG
  AGGGCAGCAGCGCCTTTCTGGAGTGTGAGCCCCGCTCGCTGCAGGCGCGCGTGGAGTGGACTTTCCAGCG
  CGCAGGGGTGACAGCCCACACCCAGGTGCTGGCAGAGGAGCGCACCGAGCGCACCGCCCGGGGACTACTG
  CTGCGCAGGCTGCGGCGCCGGGACTCGGGCGTGTACTTGTGCGCCGCCGTCGAGCAGGGCTTTACGCAAC
  CGCTGCGTCGCCTGTCGCTGCACGTGTTGAGTGCTACGCAGGCCGAACGACTGGCGCGGGCCGAGGAGGC
  TGCGCCCGCCGCGCCGCCGGGCCCCAAACTCTGGTACCGGGACTTTCTGCAGCTGGTGGAGCCGGGCGGA
  GGTGGCAGCGCGAACTCCCTGCGCATGTGCCGCCCGCAGCCTGCGCTGCAGTCACTGCCCCTGGAGTCGC
  GGAGAAAGGGCCGTAACCGGAGGACCCACGCCCCTGAGCCTCGCGCTGAGCGGGGGCCGCGCAGCGCAAC
  GCACTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223532 representing NM_004636
  Red=Cloning site Green=Tags(s)
  
  MGRAGAAAVIPGLALLWAVGLGSAAPSPPRLRLSFQELQAWHGLQTFSLERTCCYQALLVDEERGRLFVG
  AENHVASLNLDNISKRAKKLAWPAPVEWREECNWAGKDIGTECMNFVKLLHAYNRTHLLACGTGAFHPTC
  AFVEVGHRAEEPVLRLDPGRIEDGKGKSPYDPRHRAASVLVGEELYSGVAADLMGRDFTIFRSLGQRPSL
  RTEPHDSRWLNEPKFVKVFWIPESENPDDDKIYFFFRETAVEAAPALGRLSVSRVGQICRNDVGGQRSLV
  NKWTTFLKARLVCSVPGVEGDTHFDQLQDVFLLSSRDHRTPLLYAVFSTSSSIFQGSAVCVYSMNDVRRA
  FLGPFAHKEGPMHQWVSYQGRVPYPRPGMCPSKTFGTFSSTKDFPDDVIQFARNHPLMYNSVLPTGGRPL
  FLQVGANYTFTQIAADRVAAADGHYDVLFIGTDVGTVLKVISVPKGSRPSAEGLLLEELHVFEDSAAVTS
  MQISSKRHQLYVASRSAVAQIALHRCAAHGRVCTECCLARDPYCAWDGVACTRFQPSAKRRFRRQDVRNG
  DPSTLCSGDSSRPALLEHKVFGVEGSSAFLECEPRSLQARVEWTFQRAGVTAHTQVLAEERTERTARGLL
  LRRLRRRDSGVYLCAAVEQGFTQPLRRLSLHVLSATQAERLARAEEAAPAAPPGPKLWYRDFLQLVEPGG
  GGSANSLRMCRPQPALQSLPLESRRKGRNRRTHAPEPRAERGPRSATHW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004636
• ORF Size: 2247 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_004636.1, NM_004636.2, NM_004636.3, NP_004627.1
• RefSeq Size: 2989
• RefSeq ORF: 2250
• Locus ID: 7869
• Domains: Sema, IG, PSI
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Axon guidance
• MW: 83.12 kDa
• Gene Summary: The protein encoded by this gene belongs to the class-3 semaphorin/collapsin family, whose members function in growth cone guidance during neuronal development. This family member inhibits axonal extension and has been shown to act as a tumor suppressor by inducing apoptosis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]