GAS2 (NM_005256) Human Tagged ORF Clone
CAT#: RC223624
GAS2 (Myc-DDK-tagged)-Human growth arrest-specific 2 (GAS2), transcript variant 1
"NM_005256" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | GAS2 |
Synonyms | GAS-2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC223624 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCACTGCTCTGAGCCCAAAGGTACGCAGTGGACCTGGCCTCTCTGATATGCATCAGTATAGCCAAT GGCTAGCCAGCAGACATGAAGCTAATTTGCTACCAATGAAAGAAGATCTGGCCTTGTGGTTAACCAATCT ATTAGGGAAGGAGATTACAGCAGAAACTTTTATGGAGAAGTTGGACAATGGTGCCTTGCTCTGTCAACTT GCAGAAACTATGCAGGAGAAATTCAAGGAGAGCATGGATGCTAACAAGCCCACAAAGAATCTACCGTTGA AGAAGATCCCATGCAAAACCAGTGCACCCTCGGGCTCCTTTTTTGCCAGAGACAATACAGCAAATTTCTT ATCCTGGTGCCGAGATTTAGGGGTGGATGAAACGTGTCTATTTGAATCGGAAGGTTTGGTCCTCCACAAG CAACCCAGAGAAGTGTGTCTCTGTCTGCTAGAGCTTGGCCGGATTGCAGCCAGGTATGGTGTGGAGCCTC CTGGTTTGATAAAGCTGGAAAAAGAGATTGAACAAGAAGAAACACTTTCTGCCCCTTCTCCTTCACCTTC TCCTTCATCAAAGTCTTCTGGAAAAAAGAGTACAGGAAACTTACTGGATGATGCAGTGAAACGAATTTCT GAAGATCCTCCTTGCAAATGCCCAAACAAGTTCTGTGTGGAGCGGCTCTCCCAAGGAAGATACCGAGTGG GAGAAAAGATCCTCTTCATTAGGATGCTGCACAACAAACATGTCATGGTCCGTGTGGGAGGAGGCTGGGA AACTTTTGCAGGGTATTTGTTGAAACACGACCCCTGCCGAATGCTGCAGATCTCCCGTGTGGATGGCAAA ACATCCCCTATCCAAAGCAAATCTCCAACTCTAAAGGACATGAATCCAGATAACTACTTGGTGGTCTCTG CCAGTTATAAGGCTAAGAAGGAAATTAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223624 protein sequence
Red=Cloning site Green=Tags(s) MCTALSPKVRSGPGLSDMHQYSQWLASRHEANLLPMKEDLALWLTNLLGKEITAETFMEKLDNGALLCQL AETMQEKFKESMDANKPTKNLPLKKIPCKTSAPSGSFFARDNTANFLSWCRDLGVDETCLFESEGLVLHK QPREVCLCLLELGRIAARYGVEPPGLIKLEKEIEQEETLSAPSPSPSPSSKSSGKKSTGNLLDDAVKRIS EDPPCKCPNKFCVERLSQGRYRVGEKILFIRMLHNKHVMVRVGGGWETFAGYLLKHDPCRMLQISRVDGK TSPIQSKSPTLKDMNPDNYLVVSASYKAKKEIK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_005256 |
ORF Size | 939 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_005256.1, NM_005256.2, NM_005256.3, NP_005247.1 |
RefSeq Size | 2031 bp |
RefSeq ORF | 942 bp |
Locus ID | 2620 |
Cytogenetics | 11p14.3 |
Protein Families | Druggable Genome |
MW | 34.9 kDa |
Gene Summary | 'The protein encoded by this gene is a caspase-3 substrate that plays a role in regulating microfilament and cell shape changes during apoptosis. It can also modulate cell susceptibility to p53-dependent apoptosis by inhibiting calpain activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2017]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC303611 | GAS2 (untagged)-Human growth arrest-specific 2 (GAS2), transcript variant 1 |
USD 660.00 |
|
RG223624 | GAS2 (GFP-tagged) - Human growth arrest-specific 2 (GAS2), transcript variant 1 |
USD 460.00 |
|
RC223624L3 | Lenti ORF clone of Human growth arrest-specific 2 (GAS2), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC223624L4 | Lenti ORF clone of Human growth arrest-specific 2 (GAS2), transcript variant 1, mGFP tagged |
USD 620.00 |
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