NOR1 (NR4A3) (NM_173200) Human Tagged ORF Clone

CAT#: RC223629

NR4A3 (Myc-DDK-tagged)-Human nuclear receptor subfamily 4, group A, member 3 (NR4A3), transcript variant 3

Product Images

Coomassie blue staining of purified NR4A3 protein (Cat# TP323629). The protein was produced from HEK293T cells transfected with NR4A3 cDNA clone (Cat# RC223629) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NR4A3
• Synonyms: CHN; CSMF; MINOR; NOR1; TEC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223629 representing NM_173200
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATGACTCAATCAGATTTGGAAATGTGGATATGCCCTGCGTCCAAGCCCAATATAGCCCTTCCCCTC
  CAGGTTCCAGTTATGCGGCGCAGACATACAGCTCGGAATACACCACGGAGATCATGAACCCCGACTACAC
  CAAGCTGACCATGGACCTTGGCAGCACTGAGATCACGGCTACAGCCACCACGTCCCTGCCCAGCATCAGT
  ACCTTCGTGGAGGGCTACTCGAGCAACTACGAACTCAAGCCTTCCTGCGTGTACCAAATGCAGCGGCCCT
  TGATCAAAGTGGAGGAGGGGCGGGCGCCCAGCTACCATCACCATCACCACCACCACCACCACCACCACCA
  CCATCACCAGCAGCAGCATCAGCAGCCATCCATTCCTCCAGCCTCCAGCCCGGAGGACGAGGTGCTGCCC
  AGCACCTCCATGTACTTCAAGCAGTCCCCACCGTCCACCCCCACCACGCCGGCCTTCCCCCCGCAGGCGG
  GGGCGTTATGGGACGAGGCACTGCCCTCGGCGCCCGGCTGCATCGCACCCGGCCCGCTGCTGGACCCGCC
  GATGAAGGCGGTCCCCACGGTGGCCGGCGCGCGCTTCCCGCTCTTCCACTTCAAGCCCTCGCCGCCGCAT
  CCCCCCGCGCCCAGCCCGGCCGGCGGCCACCACCTCGGCTACGACCCGACGGCCGCTGCCGCGCTCAGCC
  TGCCGCTGGGAGCCGCAGCCGCCGCGGGCAGCCAGGCCGCCGCGCTTGAGAGCCACCCGTACGGGCTGCC
  GCTGGCCAAGAGGGCGGCCCCGCTGGCCTTCCCGCCTCTCGGCCTCACGCCCTCCCCTACCGCGTCCAGC
  CTGCTGGGCGAGAGTCCCAGCCTGCCGTCGCCGCCCAGCAGGAGCTCGTCGTCTGGCGAGGGCACGTGTG
  CCGTGTGCGGGGACAACGCCGCCTGCCAGCACTACGGCGTGCGAACCTGCGAGGGCTGCAAGGGCTTTTT
  CAAGAGAACAGTGCAGAAAAATGCAAAATATGTTTGCCTGGCAAATAAAAACTGCCCAGTAGACAAGAGA
  CGTCGAAACCGATGTCAGTACTGTCGATTTCAGAAGTGTCTCAGTGTTGGAATGGTAAAAGAAGTTGTCC
  GTACAGATAGTCTGAAAGGGAGGAGAGGTCGTCTGCCTTCCAAACCAAAGAGCCCATTACAACAGGAACC
  TTCTCAGCCCTCTCCACCTTCTCCTCCAATCTGCATGATGAATGCCCTTGTCCGAGCTTTAACAGACTCA
  ACACCCAGAGATCTTGATTATTCCAGATACTGTCCCACTGACCAGGCTGCTGCAGGCACAGATGCTGAGC
  ATGTGCAACAATTCTACAACCTCCTGACAGCCTCCATTGATGTATCCAGAAGCTGGGCAGAAAAGATTCC
  GGGATTTACTGATCTCCCCAAAGAAGATCAGACATTACTTATTGAATCAGCCTTTTTGGAGCTGTTTGTC
  CTCAGACTTTCCATCAGGTCAAACACTGCTGAAGATAAGTTTGTGTTCTGCAATGGACTTGTCCTGCATC
  GACTTCAGTGCCTTCGTGGATTTGGGGAGTGGCTCGACTCTATTAAAGACTTTTCCTTAAATTTGCAGAG
  CCTGAACCTTGATATCCAAGCCTTAGCCTGCCTGTCAGCACTGAGCATGATCACAGAAAGACATGGGTTA
  AAAGAACCAAAGAGAGTCGAAGAGCTATGCAACAAGATCACAAGCAGTTTAAAAGACCACCAGAGTAAGG
  GACAGGCTCTGGAGCCCACCGAGTCCAAGGTCCTGGGTGCCCTGGTAGAACTGAGGAAGATCTGCACCCT
  GGGCCTCCAGCGCATCTTCTACCTGAAGCTGGAAGACTTGGTGTCTCCACCTTCCATCATTGACAAGCTC
  TTCCTGGACACCCTACCTTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223629 representing NM_173200
  Red=Cloning site Green=Tags(s)
  
  MHDSIRFGNVDMPCVQAQYSPSPPGSSYAAQTYSSEYTTEIMNPDYTKLTMDLGSTEITATATTSLPSIS
  TFVEGYSSNYELKPSCVYQMQRPLIKVEEGRAPSYHHHHHHHHHHHHHHQQQHQQPSIPPASSPEDEVLP
  STSMYFKQSPPSTPTTPAFPPQAGALWDEALPSAPGCIAPGPLLDPPMKAVPTVAGARFPLFHFKPSPPH
  PPAPSPAGGHHLGYDPTAAAALSLPLGAAAAAGSQAAALESHPYGLPLAKRAAPLAFPPLGLTPSPTASS
  LLGESPSLPSPPSRSSSSGEGTCAVCGDNAACQHYGVRTCEGCKGFFKRTVQKNAKYVCLANKNCPVDKR
  RRNRCQYCRFQKCLSVGMVKEVVRTDSLKGRRGRLPSKPKSPLQQEPSQPSPPSPPICMMNALVRALTDS
  TPRDLDYSRYCPTDQAAAGTDAEHVQQFYNLLTASIDVSRSWAEKIPGFTDLPKEDQTLLIESAFLELFV
  LRLSIRSNTAEDKFVFCNGLVLHRLQCLRGFGEWLDSIKDFSLNLQSLNLDIQALACLSALSMITERHGL
  KEPKRVEELCNKITSSLKDHQSKGQALEPTESKVLGALVELRKICTLGLQRIFYLKLEDLVSPPSIIDKL
  FLDTLPF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173200
• ORF Size: 1911 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173200.1, NM_173200.2, NP_775292.1
• RefSeq Size: 4983
• RefSeq ORF: 1914
• Locus ID: 8013
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• MW: 69.3 kDa
• Gene Summary: This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcriptional activator. The protein can efficiently bind the NGFI-B Response Element (NBRE). Three different versions of extraskeletal myxoid chondrosarcomas (EMCs) are the result of reciprocal translocations between this gene and other genes. The translocation breakpoints are associated with Nuclear Receptor Subfamily 4, Group A, Member 3 (on chromosome 9) and either Ewing Sarcome Breakpoint Region 1 (on chromosome 22), RNA Polymerase II, TATA Box-Binding Protein-Associated Factor, 68-KD (on chromosome 17), or Transcription factor 12 (on chromosome 15). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]