Metabotropic Glutamate Receptor 4 (GRM4) (NM_000841) Human Tagged ORF Clone

CAT#: RC223673

GRM4 (Myc-DDK-tagged)-Human glutamate receptor, metabotropic 4 (GRM4)

Product Images

Western blot validation of overexpression lysate (Cat# LY400298) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223673 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRM4
• Synonyms: GPRC1D; mGlu4; MGLUR4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223673 representing NM_000841
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGGGAAGAGAGGCTTGGGCTGGTGGTGGGCCCGGCTGCCCCTTTGCCTGCTCCTCAGCCTTTACG
  GCCCCTGGATGCCTTCCTCCCTGGGAAAGCCCAAAGGCCACCCTCACATGAATTCCATCCGCATAGATGG
  GGACATCACACTGGGAGGCCTGTTCCCGGTGCATGGCCGGGGCTCAGAGGGCAAGCCCTGTGGAGAACTT
  AAGAAGGAAAAGGGCATCCACCGGCTGGAGGCCATGCTGTTCGCCCTGGATCGCATCAACAACGACCCGG
  ACCTGCTGCCTAACATCACGCTGGGCGCCCGCATTCTGGACACCTGCTCCAGGGACACCCATGCCCTCGA
  GCAGTCGCTGACCTTTGTGCAGGCGCTCATCGAGAAGGATGGCACAGAGGTCCGCTGTGGCAGTGGCGGC
  CCACCCATCATCACCAAGCCTGAACGTGTGGTGGGTGTCATCGGTGCTTCAGGGAGCTCGGTCTCCATCA
  TGGTGGCCAACATCCTTCGCCTCTTCAAGATACCCCAGATCAGCTACGCCTCCACAGCGCCAGACCTGAG
  TGACAACAGCCGCTACGATTTCTTCTCCCGCGTGGTGCCCTCGGACACGTACCAGGCCCAGGCCATGGTG
  GACATCGTCCGTGCCCTCAAGTGGAACTATGTGTCCACAGTGGCCTCGGAGGGCAGCTATGGTGAGAGCG
  GTGTGGAGGCCTTCATCCAGAAGTCCCGTGAGGACGGGGGCGTGTGCATCGCCCAGTCGGTGAAGATACC
  ACGGGAGCCCAAGGCAGGCGAGTTCGACAAGATCATCCGCCGCCTCCTGGAGACTTCGAACGCCAGGGCA
  GTCATCATCTTTGCCAACGAGGATGACATCAGGCGTGTGCTGGAGGCAGCACGAAGGGCCAACCAGACAG
  GCCATTTCTTCTGGATGGGCTCTGACAGCTGGGGCTCCAAGATTGCACCTGTGCTGCACCTGGAGGAGGT
  GGCTGAGGGTGCTGTCACGATCCTCCCCAAGAGGATGTCCGTACGAGGCTTCGACCGCTACTTCTCCAGC
  CGCACGCTGGACAACAACCGGCGCAACATCTGGTTTGCCGAGTTCTGGGAGGACAACTTCCACTGCAAGC
  TGAGCCGCCACGCCCTCAAGAAGGGCAGCCACGTCAAGAAGTGCACCAACCGTGAGCGAATTGGGCAGGA
  TTCAGCTTATGAGCAGGAGGGGAAGGTGCAGTTTGTGATCGATGCCGTGTACGCCATGGGCCACGCGCTG
  CACGCCATGCACCGTGACCTGTGTCCCGGCCGCGTGGGGCTCTGCCCGCGCATGGACCCTGTAGATGGCA
  CCCAGCTGCTTAAGTACATCCGAAACGTCAACTTCTCAGGCATCGCAGGGAACCCTGTGACCTTCAATGA
  GAATGGAGATGCGCCTGGGCGCTATGACATCTACCAATACCAGCTGCGCAACGATTCTGCCGAGTACAAG
  GTCATTGGCTCCTGGACTGACCACCTGCACCTTAGAATAGAGCGGATGCACTGGCCGGGGAGCGGGCAGC
  AGCTGCCCCGCTCCATCTGCAGCCTGCCCTGCCAACCGGGTGAGCGGAAGAAGACAGTGAAGGGCATGCC
  TTGCTGCTGGCACTGCGAGCCTTGCACAGGGTACCAGTACCAGGTGGACCGCTACACCTGTAAGACGTGT
  CCCTATGACATGCGGCCCACAGAGAACCGCACGGGCTGCCGGCCCATCCCCATCATCAAGCTTGAGTGGG
  GCTCGCCCTGGGCCGTGCTGCCCCTCTTCCTGGCCGTGGTGGGCATCGCTGCCACGTTGTTCGTGGTGAT
  CACCTTTGTGCGCTACAACGACACGCCCATCGTCAAGGCCTCGGGCCGTGAACTGAGCTACGTGCTGCTG
  GCAGGCATCTTCCTGTGCTATGCCACCACCTTCCTCATGATCGCTGAGCCCGACCTTGGCACCTGCTCGC
  TGCGCCGAATCTTCCTGGGACTAGGGATGAGCATCAGCTATGCAGCCCTGCTCACCAAGACCAACCGCAT
  CTACCGCATCTTCGAGCAGGGCAAGCGCTCGGTCAGTGCCCCACGCTTCATCAGCCCCGCCTCACAGCTG
  GCCATCACCTTCAGCCTCATCTCGCTGCAGCTGCTGGGCATCTGTGTGTGGTTTGTGGTGGACCCCTCCC
  ACTCGGTGGTGGACTTCCAGGACCAGCGGACACTCGACCCCCGCTTCGCCAGGGGTGTGCTCAAGTGTGA
  CATCTCGGACCTGTCGCTCATCTGCCTGCTGGGCTACAGCATGCTGCTCATGGTCACGTGCACCGTGTAT
  GCCATCAAGACACGCGGCGTGCCCGAGACCTTCAATGAGGCCAAGCCCATTGGCTTCACCATGTACACCA
  CTTGCATCGTCTGGCTGGCCTTCATCCCCATCTTCTTTGGCACCTCGCAGTCGGCCGACAAGCTGTACAT
  CCAGACGACGACGCTGACGGTCTCGGTGAGTCTGAGCGCCTCGGTGTCCCTGGGAATGCTCTACATGCCC
  AAAGTCTACATCATCCTCTTCCACCCGGAGCAGAATGTGCCCAAGCGCAAGCGCAGCCTCAAAGCCGTCG
  TTACGGCGGCCACCATGTCCAACAAGTTCACGCAGAAGGGCAACTTCCGGCCCAACGGAGAGGCCAAGTC
  TGAGCTCTGCGAGAACCTTGAGGCCCCAGCGCTGGCCACCAAACAGACTTACGTCACTTACACCAACCAT
  GCAATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223673 representing NM_000841
  Red=Cloning site Green=Tags(s)
  
  MPGKRGLGWWWARLPLCLLLSLYGPWMPSSLGKPKGHPHMNSIRIDGDITLGGLFPVHGRGSEGKPCGEL
  KKEKGIHRLEAMLFALDRINNDPDLLPNITLGARILDTCSRDTHALEQSLTFVQALIEKDGTEVRCGSGG
  PPIITKPERVVGVIGASGSSVSIMVANILRLFKIPQISYASTAPDLSDNSRYDFFSRVVPSDTYQAQAMV
  DIVRALKWNYVSTVASEGSYGESGVEAFIQKSREDGGVCIAQSVKIPREPKAGEFDKIIRRLLETSNARA
  VIIFANEDDIRRVLEAARRANQTGHFFWMGSDSWGSKIAPVLHLEEVAEGAVTILPKRMSVRGFDRYFSS
  RTLDNNRRNIWFAEFWEDNFHCKLSRHALKKGSHVKKCTNRERIGQDSAYEQEGKVQFVIDAVYAMGHAL
  HAMHRDLCPGRVGLCPRMDPVDGTQLLKYIRNVNFSGIAGNPVTFNENGDAPGRYDIYQYQLRNDSAEYK
  VIGSWTDHLHLRIERMHWPGSGQQLPRSICSLPCQPGERKKTVKGMPCCWHCEPCTGYQYQVDRYTCKTC
  PYDMRPTENRTGCRPIPIIKLEWGSPWAVLPLFLAVVGIAATLFVVITFVRYNDTPIVKASGRELSYVLL
  AGIFLCYATTFLMIAEPDLGTCSLRRIFLGLGMSISYAALLTKTNRIYRIFEQGKRSVSAPRFISPASQL
  AITFSLISLQLLGICVWFVVDPSHSVVDFQDQRTLDPRFARGVLKCDISDLSLICLLGYSMLLMVTCTVY
  AIKTRGVPETFNEAKPIGFTMYTTCIVWLAFIPIFFGTSQSADKLYIQTTTLTVSVSLSASVSLGMLYMP
  KVYIILFHPEQNVPKRKRSLKAVVTAATMSNKFTQKGNFRPNGEAKSELCENLEAPALATKQTYVTYTNH
  AI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000841
• ORF Size: 2736 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000841.1, NM_000841.2, NM_000841.3, NP_000832.1
• RefSeq Size: 3884 bp
• RefSeq ORF: 2739 bp
• Locus ID: 2914
• Cytogenetics: 6p21.31
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction, Taste transduction
• MW: 101.87 kDa
• Gene Summary: 'L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]'