SLCO3A1 (NM_013272) Human Tagged ORF Clone

CAT#: RC223712

SLCO3A1 (Myc-DDK-tagged)-Human solute carrier organic anion transporter family, member 3A1 (SLCO3A1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLCO3A1
• Synonyms: OATP-D; OATP-RP3; OATP3A1; OATPD; OATPRP3; SLC21A11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223712 representing NM_013272
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGGAAGAAGCCGGGCGGTTCGTCGGGCGGCGGCCGGAGCGGCGAGCTGCAGGGGGACGAGGCGC
  AGAGGAACAAGAAAAAGAAAAAGAAGGTGTCCTGCTTTTCCAACATCAAGATCTTCCTGGTGTCCGAGTG
  CGCCCTGATGCTGGCGCAGGGCACGGTGGGCGCCTACCTGGTGAGCGTCCTGACCACCCTGGAGCGTAGG
  TTCAACCTGCAGAGCGCTGACGTGGGTGTGATCGCTAGCAGCTTCGAGATCGGGAACCTGGCGCTCATCC
  TCTTCGTGAGCTACTTCGGGGCACGCGGGCACCGGCCGCGCCTGATCGGCTGCGGCGGCATCGTCATGGC
  GCTGGGCGCGCTGCTGTCGGCGCTGCCCGAGTTCCTGACCCACCAGTACAAGTACGAGGCGGGCGAGATC
  CGCTGGGGCGCCGAGGGCCGCGACGTCTGCGCAGCCAACGGCTCGGGCGGCGACGAGGGGCCCGACCCCG
  ACCTCATCTGCCGCAACCGGACGGCTACCAACATGATGTACTTGCTGCTCATTGGGGCCCAGGTGCTCCT
  GGGCATCGGTGCTACCCCTGTGCAGCCCCTGGGCGTCTCCTACATCGACGACCACGTGCGGAGGAAGGAC
  TCCTCGCTCTATATAGGAATCCTGTTCACGATGCTGGTATTTGGACCAGCCTGCGGGTTTATCCTGGGCT
  CTTTCTGTACCAAAATCTACGTGGATGCAGTCTTCATTGACACAAGTAACCTGGACATCACTCCGGACGA
  CCCCCGCTGGATCGGAGCCTGGTGGGGTGGCTTTCTGCTCTGCGGTGCCTTACTCTTCTTCTCTTCCCTC
  TTGATGTTTGGGTTTCCACAGTCCCTGCCCCCGCACTCAGACCCCGCCATGGAAAGCGAGCAGGCCATGC
  TCTCCGAAAGAGAATACGAGAGACCCAAGCCCAGCAACGGGGTCCTGAGGCACCCCCTGGAGCCAGACAG
  CAGTGCCTCCTGTTTCCAGCAGCTGAGAGTGATCCCGAAGGTCACCAAGCACCTGCTCTCAAACCCTGTG
  TTCACCTGCATCATCCTGGCCGCCTGCATGGAGATTGCAGTGGTGGCTGGCTTCGCTGCCTTTTTGGGGA
  AGTACCTGGAGCAGCAGTTTAACCTCACCACCTCTTCTGCCAACCAGCTGCTTGGGATGACTGCGATCCC
  GTGTGCTTGTCTGGGTATCTTCCTGGGAGGTCTTTTGGTGAAGAAGCTCAGCCTGTCTGCCCTGGGGGCC
  ATTCGGATGGCCATGCTCGTCAACCTGGTGTCCACTGCTTGCTACGTCTCCTTCCTCTTCCTGGGCTGCG
  ACACTGGCCCTGTGGCTGGGGTTACTGTTCCCTATGGAAACAGCACAGCACCTGGCTCAGCCCTGGACCC
  CTACTCGCCCTGCAATAATAACTGTGAATGCCAAACCGATTCCTTCACTCCAGTGTGTGGGGCAGATGGC
  ATCACCTACCTGTCTGCCTGCTTTGCTGGCTGCAACAGCACGAATCTCACGGGCTGTGCGTGCCTCACCA
  CCGTCCCTGCTGAGAACGCAACCGTGGTTCCTGGAAAATGCCCCAGTCCTGGGTGCCAAGAGGCCTTCCT
  CACTTTCCTCTGTGTGATGTGTATCTGCAGCCTGATCGGTGCCATGGCACAGACACCCTCAGTCATCATC
  CTCATCAGGACAGTCAGCCCTGAACTCAAGTCTTACGCTTTGGGAGTTCTTTTTCTCCTCCTTCGTTTGT
  TGGGCTTCATCCCTCCACCCCTCATCTTCGGGGCTGGCATCGACTCCACCTGCCTGTTCTGGAGCACGTT
  CTGTGGGGAGCAAGGCGCCTGCGTCCTCTACGACAATGTGGTCTACCGATACCTGTATGTCAGCATCGCC
  ATCGCGCTCAAATCCTTCGCCTTCATCCTGTACACCACCACGTGGCAGTGCCTGAGGAAAAACTATAAAC
  GCTACATCAAAAACCACGAGGGCGGGCTGAGCACCAGTGAGTTCTTTGCCTCTACTCTGACCCTAGACAA
  CCTGGGGAGGGACCCTGTGCCCGCAAACCAGACACATAGGACAAAGTTTATCTATAACCTGGAAGACCAT
  GAGTGGTGTGAAAACATGGAGTCCGTTTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223712 representing NM_013272
  Red=Cloning site Green=Tags(s)
  
  MQGKKPGGSSGGGRSGELQGDEAQRNKKKKKKVSCFSNIKIFLVSECALMLAQGTVGAYLVSVLTTLERR
  FNLQSADVGVIASSFEIGNLALILFVSYFGARGHRPRLIGCGGIVMALGALLSALPEFLTHQYKYEAGEI
  RWGAEGRDVCAANGSGGDEGPDPDLICRNRTATNMMYLLLIGAQVLLGIGATPVQPLGVSYIDDHVRRKD
  SSLYIGILFTMLVFGPACGFILGSFCTKIYVDAVFIDTSNLDITPDDPRWIGAWWGGFLLCGALLFFSSL
  LMFGFPQSLPPHSDPAMESEQAMLSEREYERPKPSNGVLRHPLEPDSSASCFQQLRVIPKVTKHLLSNPV
  FTCIILAACMEIAVVAGFAAFLGKYLEQQFNLTTSSANQLLGMTAIPCACLGIFLGGLLVKKLSLSALGA
  IRMAMLVNLVSTACYVSFLFLGCDTGPVAGVTVPYGNSTAPGSALDPYSPCNNNCECQTDSFTPVCGADG
  ITYLSACFAGCNSTNLTGCACLTTVPAENATVVPGKCPSPGCQEAFLTFLCVMCICSLIGAMAQTPSVII
  LIRTVSPELKSYALGVLFLLLRLLGFIPPPLIFGAGIDSTCLFWSTFCGEQGACVLYDNVVYRYLYVSIA
  IALKSFAFILYTTTWQCLRKNYKRYIKNHEGGLSTSEFFASTLTLDNLGRDPVPANQTHRTKFIYNLEDH
  EWCENMESVL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_013272
• ORF Size: 2130 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013272.1, NM_013272.2, NM_013272.3, NP_037404.1
• RefSeq Size: 2740 bp
• RefSeq ORF: 2133 bp
• Locus ID: 28232
• Domains: OATP_N, OATP_C
• Protein Families: Transmembrane
• MW: 76.4 kDa