BCMO1 (BCO1) (NM_017429) Human Tagged ORF Clone

CAT#: RC223790

BCMO1 (Myc-DDK-tagged)-Human beta-carotene 15,15'-monooxygenase 1 (BCMO1)

Product Images

Western blot validation of overexpression lysate (Cat# LY413785) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223790 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified BCO1 protein (Cat# TP323790). The protein was produced from HEK293T cells transfected with BCO1 cDNA clone (Cat# RC223790) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BCO1
• Synonyms: BCDO; BCDO1; BCMO; BCMO1; BCO
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223790 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATATAATATTTGGCAGGAATAGGAAAGAACAGCTGGAGCCTGTGAGGGCCAAAGTGACAGGCAAGA
  TTCCAGCATGGCTGCAGGGAACCCTGCTCCGCAACGGGCCTGGGATGCACACAGTTGGGGAGTCCAGATA
  CAACCATTGGTTCGACGGCCTTGCCCTGCTCCACAGCTTCACCATCAGAGACGGTGAAGTCTATTACAGG
  AGCAAATACCTGAGAAGCGATACCTACAACACCAATATTGAGGCAAACAGGATTGTGGTGTCTGAGTTTG
  GAACAATGGCCTATCCGGACCCCTGCAAAAACATATTTTCCAAAGCTTTCTCCTACTTGTCTCACACCAT
  CCCCGATTTCACCGACAACTGCCTGATCAACATCATGAAGTGCGGAGAAGACTTCTACGCGACCTCAGAG
  ACCAATTACATCAGGAAAATCAACCCACAGACTCTGGAAACCCTGGAGAAGGTTGATTATCGTAAATACG
  TGGCGGTAAATCTGGCAACGTCACATCCCCATTATGATGAGGCTGGAAATGTTCTAAACATGGGCACATC
  CATTGTGGAAAAGGGGAAGACAAAGTATGTGATTTTTAAGATCCCTGCCACAGTACCAGAGGGCAAGAAG
  CAGGGGAAGAGCCCCTGGAAGCACACAGAGGTGTTCTGCTCCATCCCATCCCGCTCCCTGCTCTCCCCAA
  GCTACTACCACAGCTTTGGAGTCACCGAGAACTATGTCATCTTCCTTGAGCAGCCTTTCAGGTTGGATAT
  TCTCAAGATGGCAACCGCATACATCCGGAGTATGAGCTGGGCCTCCTGCCTGGCTTTCCACAGGGAGGAG
  AAGACTTATATCCACATCATCGACCAAAGGACCAGGCAGCCTGTGCAGACCAAGTTTTACACAGACGCCA
  TGGTGGTCTTCCATCACGTCAACGCCTACGAAGAGGACGGCTGCATCGTGTTTGACGTCATTGCCTACGA
  GGACAACAGCCTCTACCAGCTCTTCTACCTGGCCAACCTGAACCAGGACTTCAAGGAGAACTCCAGGCTC
  ACCTCGGTCCCCACCCTCAGGAGGTTTGCCGTGCCCCTCCACGTGGACAAGAATGCAGAAGTGGGCACAA
  ATTTAATCAAAGTGGCATCTACAACAGCCACGGCCCTGAAGGAAGAAGATGGCCAAGTCTACTGCCAGCC
  GGAATTTCTTTATGAAGGCTTAGAGCTTCCACGGGTCAATTATGCTCACAATGGAAAGCAATACCGATAT
  GTCTTTGCTACAGGAGTTCAGTGGAGTCCAATCCCAACCAAGATAATAAAATATGACATTCTCACAAAGT
  CATCCTTAAAATGGAGAGAGGACGACTGCTGGCCAGCGGAACCCCTGTTTGTGCCCGCGCCAGGTGCCAA
  GGATGAGGATGACGGAGTAATCTTATCAGCCATTGTCTCTACTGATCCCCAAAAGCTGCCTTTTCTGCTC
  ATTCTGGATGCCAAAAGCTTTACGGAATTGGCCCGTGCCTCTGTTGATGTCGATATGCACATGGATCTCC
  ATGGATTATTCATTACAGACATGGACTGGGACACAAAAAAGCAGGCCGCTTCTGAGGAACAGCGGGACAG
  GGCTTCCGACTGCCACGGGGCTCCTCTGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223790 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDIIFGRNRKEQLEPVRAKVTGKIPAWLQGTLLRNGPGMHTVGESRYNHWFDGLALLHSFTIRDGEVYYR
  SKYLRSDTYNTNIEANRIVVSEFGTMAYPDPCKNIFSKAFSYLSHTIPDFTDNCLINIMKCGEDFYATSE
  TNYIRKINPQTLETLEKVDYRKYVAVNLATSHPHYDEAGNVLNMGTSIVEKGKTKYVIFKIPATVPEGKK
  QGKSPWKHTEVFCSIPSRSLLSPSYYHSFGVTENYVIFLEQPFRLDILKMATAYIRSMSWASCLAFHREE
  KTYIHIIDQRTRQPVQTKFYTDAMVVFHHVNAYEEDGCIVFDVIAYEDNSLYQLFYLANLNQDFKENSRL
  TSVPTLRRFAVPLHVDKNAEVGTNLIKVASTTATALKEEDGQVYCQPEFLYEGLELPRVNYAHNGKQYRY
  VFATGVQWSPIPTKIIKYDILTKSSLKWREDDCWPAEPLFVPAPGAKDEDDGVILSAIVSTDPQKLPFLL
  ILDAKSFTELARASVDVDMHMDLHGLFITDMDWDTKKQAASEEQRDRASDCHGAPLT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017429
• ORF Size: 1641 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017429.1, NM_017429.2, NP_059125.2
• RefSeq Size: 2446 bp
• RefSeq ORF: 1644 bp
• Locus ID: 53630
• Protein Pathways: Metabolic pathways, Retinol metabolism
• MW: 62.6 kDa
• Gene Summary: Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of beta,beta-carotene into two retinal molecules. [provided by RefSeq, Jul 2008]