RHOBTB1 (NM_001032380) Human Tagged ORF Clone
CAT#: RC223885
RHOBTB1 (Myc-DDK-tagged)-Human Rho-related BTB domain containing 1 (RHOBTB1), transcript variant 3
"NM_001032380" in other vectors (3)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | RHOBTB1 |
Synonyms | 1700008H16Rik; 3110048G13Rik; AI173445; AI573858; AV350930; KIAA0740, MGC33059, MGC33841; MGC107538; Rho-related BTB domain containing 1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC223885 representing NM_001032380
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGCTGACATGGACTACGAAAGACCCAACGTTGAAACTATCAAATGTGTGGTCGTGGGTGACAATG CCGTGGGGAAGACGCGCTTGATCTGTGCCAGGGCGTGCAACACCACACTCACGCAGTATCAGCTGCTGGC CACCCACGTGCCAACAGTGTGGGCGATTGACCAGTACCGCGTGTGCCAGGAGGTCTTGGAGCGTTCTCGG GATGTTGTTGATGAAGTGAGTGTTTCTCTCAGGCTTTGGGATACTTTTGGTGATCATCACAAAGACAGAC GCTTTGCATATGGCAGGTCTGATGTTGTGGTCCTCTGTTTTTCGATTGCTAATCCCAATTCCCTAAATCA TGTGAAAAGCATGTGGTATCCAGAAATCAAGCACTTTTGCCCTCGAACACCCGTTATCCTTGTTGGGTGC CAGCTTGATCTCCGCTATGCCGACCTGGAAGCTGTTAATCGAGCCAGGCGCCCGTTAGCAAGGCCCATAA AGAGAGGGGATATTTTGCCCCCAGAAAAAGGCCGAGAGGTAGCAAAGGAACTTGGCTTACCATACTATGA AACAAGCGTGTTTGACCAGTTTGGTATCAAGGATGTGTTTGACAATGCAATCCGAGCAGCGCTGATTTCC CGCAGGCACCTGCAATTCTGGAAATCCCACCTAAAGAAAGTCCAGAAACCTTTACTTCAGGCACCCTTCC TACCTCCAAAAGCCCCTCCACCGGTCATCAAAATTCCAGAGTGTCCTTCCATGGGGACAAATGAAGCTGC CTGTTTACTGGACAATCCTCTATGTGCCGATGTTCTGTTCATCCTTCAGGACCAGGAACACATCTTTGCA CATCGAATTTACCTCGCTACCTCTTCTTCCAAATTTTATGATCTGTTTTTAATGGAATGTGAAGAATCCC CAAATGGGAGTGAAGGAGCCTGTGAGAAAGAGAAGCAGAGCAGAGATTTCCAGGGGCGGATATTGAGTGT CGACCCAGAGGAAGAAAGGGAGGAGGGCCCGCCTAGGATTCCTCAGGCCGACCAGTGGAAGTCTTCAAAC AAGAGCCTGGTGGAGGCTCTGGGGCTGGAAGCCGAGGGTGCAGTTCCTGAGACACAGACTTTGACCGGAT GGAGTAAGGGGTTCATTGGCATGCACAGGGAAATGCAAGTCAACCCCATTTCAAAGCGGATGGGGCCCAT GACTGTGGTCAGGATGGACGCTTCAGTCCAGCCAGGCCCTTTTCGGACCCTGCTCCAGTTTCTTTATACG GGACAACTGGATGAAAAGGAAAAGGATTTGGTGGGCCTGGCTCAGATCGCAGAGGTCCTCGAGATGTTCG ATTTGAGGATGATGGTGGAAAACATCATGAACAAGGAAGCCTTCATGAACCAGGAGATTACGAAAGCCTT TCACGTAAGGAAAGCCAATCGGATAAAAGAGTGTCTCAGCAAGGGAACGTTCTCGGACGTGACATTTAAA TTGGACGATGGAGCCATCAGTGCCCACAAGCCGCTGCTGATCTGTAGCTGTGAGTGGATGGCAGCCATGT TCGGGGGGTCATTTGTGGAAAGTGCCAACAGTGAGGTGTATCTCCCGAACATAAACAAGATATCAATGCA AGCAGTATTGGATTATCTCTATACCAAGCAGTTGTCTCCTAACTTGGATCTGGACCCGCTGGAATTAATT GCCTTGGCAAACAGATTTTGCCTGCCACACTTGGTTGCACTTGCAGAACAGCATGCCGTTCAGGAGTTGA CCAAAGCCGCCACGAGTGGCGTGGGCATTGACGGAGAAGTGCTCTCTTACTTGGAATTGGCTCAGTTTCA CAATGCCCACCAGTTGGCCGCCTGGTGTTTGCACCACATCTGCACCAACTACAACAGTGTATGCTCCAAG TTCCGTAAGGAAATCAAATCAAAATCTGCAGACAACCAGGAATACTTCGAGCGGCACCGCTGGCCCCCTG TGTGGTACCTGAAGGAAGAAGATCACTACCAGCGTGTGAAAAGGGAACGAGAGAAGGAAGATATTGCACT AAATAAGCATCGCTCAAGACGAAAGTGGTGCTTCTGGAATTCATCTCCAGCAGTGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC223885 representing NM_001032380
Red=Cloning site Green=Tags(s) MDADMDYERPNVETIKCVVVGDNAVGKTRLICARACNTTLTQYQLLATHVPTVWAIDQYRVCQEVLERSR DVVDEVSVSLRLWDTFGDHHKDRRFAYGRSDVVVLCFSIANPNSLNHVKSMWYPEIKHFCPRTPVILVGC QLDLRYADLEAVNRARRPLARPIKRGDILPPEKGREVAKELGLPYYETSVFDQFGIKDVFDNAIRAALIS RRHLQFWKSHLKKVQKPLLQAPFLPPKAPPPVIKIPECPSMGTNEAACLLDNPLCADVLFILQDQEHIFA HRIYLATSSSKFYDLFLMECEESPNGSEGACEKEKQSRDFQGRILSVDPEEEREEGPPRIPQADQWKSSN KSLVEALGLEAEGAVPETQTLTGWSKGFIGMHREMQVNPISKRMGPMTVVRMDASVQPGPFRTLLQFLYT GQLDEKEKDLVGLAQIAEVLEMFDLRMMVENIMNKEAFMNQEITKAFHVRKANRIKECLSKGTFSDVTFK LDDGAISAHKPLLICSCEWMAAMFGGSFVESANSEVYLPNINKISMQAVLDYLYTKQLSPNLDLDPLELI ALANRFCLPHLVALAEQHAVQELTKAATSGVGIDGEVLSYLELAQFHNAHQLAAWCLHHICTNYNSVCSK FRKEIKSKSADNQEYFERHRWPPVWYLKEEDHYQRVKREREKEDIALNKHRSRRKWCFWNSSPAVA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001032380 |
ORF Size | 2088 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001032380.1, NP_001027552.1 |
RefSeq Size | 4381 bp |
RefSeq ORF | 2090 bp |
Locus ID | 9886 |
MW | 79.9 kDa |
Gene Summary | The protein encoded by this gene belongs to the Rho family of the small GTPase superfamily. It contains a GTPase domain, a proline-rich region, a tandem of 2 BTB (broad complex, tramtrack, and bric-a-brac) domains, and a conserved C-terminal region. The protein plays a role in small GTPase-mediated signal transduction and the organization of the actin filament system. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RG223885 | RHOBTB1 (GFP-tagged) - Human Rho-related BTB domain containing 1 (RHOBTB1), transcript variant 3 |
USD 800.00 |
|
RC223885L3 | Lenti ORF clone of Human Rho-related BTB domain containing 1 (RHOBTB1), transcript variant 3, Myc-DDK-tagged |
USD 930.00 |
|
RC223885L4 | Lenti ORF clone of Human Rho-related BTB domain containing 1 (RHOBTB1), transcript variant 3, mGFP tagged |
USD 930.00 |
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