PP4R1 (PPP4R1) (NM_001042388) Human Tagged ORF Clone

CAT#: RC223947

PPP4R1 (Myc-DDK-tagged)-Human protein phosphatase 4, regulatory subunit 1 (PPP4R1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPP4R1
• Synonyms: MEG1; PP4(Rmeg); PP4R1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223947 representing NM_001042388
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACCTCTCGCTGCTTCAGGAGGACCTGCAGGAGGACGCAGACGGATTTGGTGTGGATGACTACA
  GCTCAGAGTCTGATGTGATTATTATACCTTCAGCCCTGGACTTTGTCTCACAAGATGAAATGTTGACGCC
  CCTGGGGAGATTGGACAAGTATGCTGCAAGTGAGAACATATTTAACAGACAAATGGTGGCCCGGAGTTTG
  CTCGATACCTTGAGGGAAGTCTGCGATGATGAAAGAGATTGTATTGCTGTTTTGGAAAGAATTAGCAGAT
  TGGCCGATGATTCAGAACCAACTGTGAGAGCGGAGCTGATGGAACAGGTGCCTCACATCGCACTGTTTTG
  TCAAGAAAACCGGCCTTCAATACCATATGCTTTTTCAAAATTCTTACTACCTATTGTGGTTAGATACCTT
  GCAGATCAGAATAATCAGGTGAGGAAAACAAGTCAGGCAGCTTTGCTGGCTCTGTTGGAGCAGGAGCTCA
  TTGAACGATTTGATGTGGAGACCAAAGTGTGCCCTGTCCTCATAGAGCTGACAGCCCCAGATAGCAATGA
  TGATGTGAAAACAGAAGCTGTGGCTATAATGTGCAAAATGGCTCCCATGGTTGGGAAGGATATTACAGAG
  CGTCTTATCCTCCCTAGGTTTTGTGAGATGTGCTGCGATTGCAGAATGTTTCACGTTCGAAAGGTCTGTG
  CTGCCAATTTTGGAGATATTTGCAGTGTAGTTGGCCAGCAAGCTACTGAAGAAATGTTGCTGCCCAGATT
  TTTCCAGCTTTGTTCTGATAATGTATGGGGAGTCCGAAAGGCTTGTGCTGAATGCTTCATGGCGGTTTCA
  TGTGCAACATGTCAAGAAATCCGACGGACCAAATTATCAGCACTTTTTATTAATTTGATCAGTGATCCTT
  CACGTTGGGTTCGCCAAGCAGCTTTTCAGTCTCTGGGACCTTTCATATCTACTTTTGCTAATCCATCTAG
  CTCAGGCCAGTATTTTAAAGAAGAAAGCAAAAGTTCAGAAGAGATGTCAGTAGAAAACAAAAATAGGACC
  AGAGATCAAGAAGCCCCAGAGGATGTACAAGTCAGGCCAGAGGATACTCCTTCAGATCTCAGTGTTAGTA
  ATTCCAGTGTCATACTGGAAAACACGATGGAAGACCATGCTGCTGAGGCATCCGGGAAGCCTCTAGGTGA
  AATTAGTGTTCCACTGGACAGCTCTTTACTTTGTACTTTGTCCTCAGAATCTCACCAGGAAGCAGCTAGT
  AATGAGAATGATAAAAAACCTGGTAACTACAAATCTATGTTACGACCAGAGGTTGGCACCACTTCACAAG
  ATTCAGCTCTCTTAGATCAGGAATTGTATAACTCCTTCCATTTCTGGAGGACTCCTCTTCCTGAAATAGA
  TCTAGACATAGAGCTTGAACAGAACTCTGGGGGAAAACCCAGCCCAGAGGGACCAGAGGAAGAATCTGAG
  GGCCCTGTGCCCAGTTCTCCAAACATCACCATGGCCACCAGAAAGGAACTGGAAGAAATGATAGAAAATC
  TAGAGCCCCACATTGATGATCCAGATGTTAAAGCACAAGTGGAAGTGCTGTCCGCTGCACTACGTGCTTC
  CAGCCTGGATGCACATGAAGAGACCATCAGTATAGAAAAGAGAAGTGATTTGCAAGATGAACTGGATATA
  AATGAGCTACCAAATTGTAAAATAAATCAAGAAGATTCTGTGCCTTTAATCAGCGATGCTGTTGAGAATA
  TGGACTCCACTCTTCACTATATTCACAGCGATTCAGACTTGAGCAACAATAGCAGTTTTAGCCCTGATGA
  GGAAAGGAGAACTAAAGTACAAGATGTTGTACCTCAGGCGTTGTTAGATCAGTATTTATCTATGACTGAC
  CCTTCTCGTGCACAGACGGTTGACACTGAAATTGCTAAGCACTGTGCATATAGCCTCCCTGGTGTGGCCT
  TGACACTCGGAAGACAGAATTGGCACTGCCTGAGAGAGACGTATGAGACTCTGGCCTCAGACATGCAGTG
  GAAAGTTCGACGAACTCTAGCATTCTCCATCCACGAGCTTGCAGTTATTCTTGGAGATCAATTGACAGCT
  GCAGATCTGGTTCCAATTTTTAATGGATTTTTAAAAGACCTCGATGAAGTCAGGATAGGTGTTCTTAAAC
  ACTTGCATGATTTTCTGAAGCTTCTTCATATTGACAAAAGAAGAGAATATCTTTATCAACTTCAGGAGTT
  TTTGGTGACAGATAATAGTAGAAATTGGCGGTTTCGAGCTGAACTGGCTGAACAGCTGATTTTACTTCTA
  GAGTTATATAGTCCCAGAGATGTTTATGACTATTTACGTCCCATTGCTCTGAATCTGTGTGCAGACAAAG
  TTTCTTCTGTTCGTTGGATTTCCTACAAGTTGGTCAGCGAGATGGTGAAGAAGCTGCACGCGGCAACACC
  ACCAACGTTCGGAGTGGACCTCATCAATGAGCTTGTGGAGAACTTTGGCAGATGTCCCAAGTGGTCTGGT
  CGGCAAGCCTTTGTCTTTGTCTGCCAGACTGTCATTGAGGATGACTGCCTTCCCATGGACCAGTTTGCTG
  TGCATCTCATGCCGCATCTGCTAACCTTAGCAAATGACAGGGTTCCTAACGTGCGAGTGCTGCTTGCAAA
  GACATTAAGACAAACTCTACTAGAAAAAGACTATTTCTTGGCCTCTGCCAGCTGCCACCAGGAGGCTGTG
  GAGCAGACCATCATGGCTCTTCAGATGGACCGTGACAGCGATGTCAAGTATTTTGCAAGCATCCACCCTG
  CCAGTACCAAAATCTCCGAAGATGCCATGAGCACAGCGTCCTCAACCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223947 representing NM_001042388
  Red=Cloning site Green=Tags(s)
  
  MADLSLLQEDLQEDADGFGVDDYSSESDVIIIPSALDFVSQDEMLTPLGRLDKYAASENIFNRQMVARSL
  LDTLREVCDDERDCIAVLERISRLADDSEPTVRAELMEQVPHIALFCQENRPSIPYAFSKFLLPIVVRYL
  ADQNNQVRKTSQAALLALLEQELIERFDVETKVCPVLIELTAPDSNDDVKTEAVAIMCKMAPMVGKDITE
  RLILPRFCEMCCDCRMFHVRKVCAANFGDICSVVGQQATEEMLLPRFFQLCSDNVWGVRKACAECFMAVS
  CATCQEIRRTKLSALFINLISDPSRWVRQAAFQSLGPFISTFANPSSSGQYFKEESKSSEEMSVENKNRT
  RDQEAPEDVQVRPEDTPSDLSVSNSSVILENTMEDHAAEASGKPLGEISVPLDSSLLCTLSSESHQEAAS
  NENDKKPGNYKSMLRPEVGTTSQDSALLDQELYNSFHFWRTPLPEIDLDIELEQNSGGKPSPEGPEEESE
  GPVPSSPNITMATRKELEEMIENLEPHIDDPDVKAQVEVLSAALRASSLDAHEETISIEKRSDLQDELDI
  NELPNCKINQEDSVPLISDAVENMDSTLHYIHSDSDLSNNSSFSPDEERRTKVQDVVPQALLDQYLSMTD
  PSRAQTVDTEIAKHCAYSLPGVALTLGRQNWHCLRETYETLASDMQWKVRRTLAFSIHELAVILGDQLTA
  ADLVPIFNGFLKDLDEVRIGVLKHLHDFLKLLHIDKRREYLYQLQEFLVTDNSRNWRFRAELAEQLILLL
  ELYSPRDVYDYLRPIALNLCADKVSSVRWISYKLVSEMVKKLHAATPPTFGVDLINELVENFGRCPKWSG
  RQAFVFVCQTVIEDDCLPMDQFAVHLMPHLLTLANDRVPNVRVLLAKTLRQTLLEKDYFLASASCHQEAV
  EQTIMALQMDRDSDVKYFASIHPASTKISEDAMSTASSTY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042388
• ORF Size: 2850 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001042388.1, NM_001042388.2, NP_001035847.1
• RefSeq Size: 3980
• RefSeq ORF: 2853
• Locus ID: 9989
• Protein Families: Druggable Genome, Phosphatase
• MW: 107 kDa
• Gene Summary: This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]