DGLUCY (NM_001102369) Human Tagged ORF Clone

CAT#: RC223957

C14orf159 (Myc-DDK-tagged)-Human chromosome 14 open reading frame 159 (C14orf159), transcript variant 5

Product Images

Western blot validation of overexpression lysate (Cat# LY420136) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223957 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DGLUCY
• Synonyms: C14orf159
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC223957 representing NM_001102369
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCTTCACACTCCACCTGAGGTCCCGCCTTCCCTCTGCCATAAGGAGTTTGATTCTACAAAAGAAAC
  CAAACATCAGAAATACATCCAGCATGGCTGGAGAGCTCCGACCAGCCAGCCTGGTGGTCCTGCCCAGGTC
  CCTTGCTCCAGCTTTTGAAAGATTCTGCCAGGTCAACACTGGTCCTCTACCCCTGCTGGGCCAGAGTGAG
  CCAGAAAAGTGGATGCTGCCCCCTCAAGGTGCTATCTCAGAGACCAGGATGGGCCATCCCCAGTTCTGGA
  AATACGAGTTCGGTGCCTGCACCGGCAGCCTGGCTTCGCTGGAGCAGTACTCGGAGCAGCTGAAGGACAT
  GGTGGCCTTCTTCCTGGGCTGCAGCTTCTCCCTGGAGGAGGCCTTGGAGAAAGCGGGGCTCCCCAGAAGA
  GACCCAGCAGGTCACAGCCAGACAACAGTGCCTTGTGTTACCCATGCTGGCTTCTGCTGCCCTCTGGTGG
  TCACGATGAGGCCCATTCCCAAGGACAAGCTGGAAGGGCTGGTGCGGGCCTGCTGCTCCCTCGGAGAACT
  GTTGGGAATCAAAGAGCTTTCCAAACCTGCCTACGGGGATGCCATGGTGTGTCCCCCAGGGGAGGTTCCA
  GTGTTCTGGCCTTCTCCGCTGACCAGTCTCGGAGCTGTCAGCAGCTGTGAGACCCCACTGGCTTTTGCCA
  GCATCCCAGGCTGCACAGTTATGACTGACCTGAAGGATGCAAAGGCTCCACCTGGTTGTCTCACCCCAGA
  GAGAATTCCAGAGGTCCATCACATTTCCCAAGATCCTCTGCACTACAGCATCGCGTCAGTCTCTGCTTCT
  CAGAAGATCAGAGAACTAGAGTCTATGATCGGCATAGACCCAGGGAACCGGGGGATTGGGCACCTGCTCT
  GTAAAGATGAGCTGCTGAAGGCCTCTCTCTCGCTGTCCCATGCCCGCTCAGTGCTCATCACCACTGGGTT
  CCCCACACATTTCAATCATGAGCCTCCAGAAGAGACAGATGGCCCACCAGGAGCTGTTGCTCTGGTTGCC
  TTCCTGCAGGCCTTGGAGAAGGAGGTCGCCATAATCGTTGACCAGAGAGCCTGGAACTTGCACCAGAAGA
  TTGTTGAAGATGCTGTTGAGCAAGGTGTTCTGAAGACGCAGATCCCGATATTAACTTACCAAGGTGGATC
  AGTGGAAGCTGCTCAGGCATTCCTGTGCAAAAATGGGGACCCGCAGACACCTAGATTTGACCACCTGGTG
  GCCATAGAGCGTGCCGGAAGAGCTGCTGATGGCAATTACTACAATGCAAGGAAGATGAACATCAAGCACT
  TGGTTGACCCCATTGACGATCTTTTTCTTGCTGCGAAGAAGATTCCTGGAATCTCATCAACTGGTGTTTC
  TAACTGGGGAGGCTATGCCCTGGCCTGCGCACTCTACATCCTGTACTCATGTGCTGTCCACAGTCAGTAC
  CTGAGGAAAGCAGTCGGACCCTCCAGGGCACCTGGAGATCAGGCCTGGACTCAGGCCCTCCCGTCGGTCA
  TTAAGGAAGAAAAAATGCTGGGCATCTTGGTGCAGCACAAAGTCCGGAGTGGCGTCTCGGGCATCGTGGG
  CATGGAGGTGGATGGGCTGCCCTTCCACAACACCCACGCCGAGATGATCCAGAAGCTGGTGGACGTCACC
  ACGGCACAGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC223957 representing NM_001102369
  Red=Cloning site Green=Tags(s)
  
  MPFTLHLRSRLPSAIRSLILQKKPNIRNTSSMAGELRPASLVVLPRSLAPAFERFCQVNTGPLPLLGQSE
  PEKWMLPPQGAISETRMGHPQFWKYEFGACTGSLASLEQYSEQLKDMVAFFLGCSFSLEEALEKAGLPRR
  DPAGHSQTTVPCVTHAGFCCPLVVTMRPIPKDKLEGLVRACCSLGELLGIKELSKPAYGDAMVCPPGEVP
  VFWPSPLTSLGAVSSCETPLAFASIPGCTVMTDLKDAKAPPGCLTPERIPEVHHISQDPLHYSIASVSAS
  QKIRELESMIGIDPGNRGIGHLLCKDELLKASLSLSHARSVLITTGFPTHFNHEPPEETDGPPGAVALVA
  FLQALEKEVAIIVDQRAWNLHQKIVEDAVEQGVLKTQIPILTYQGGSVEAAQAFLCKNGDPQTPRFDHLV
  AIERAGRAADGNYYNARKMNIKHLVDPIDDLFLAAKKIPGISSTGVSNWGGYALACALYILYSCAVHSQY
  LRKAVGPSRAPGDQAWTQALPSVIKEEKMLGILVQHKVRSGVSGIVGMEVDGLPFHNTHAEMIQKLVDVT
  TAQV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001102369
• ORF Size: 1692 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001102369.1, NM_001102369.2, NP_001095839.1
• RefSeq Size: 2763
• RefSeq ORF: 1695
• Locus ID: 80017
• MW: 60.9 kDa