OR5A2 (NM_001001954) Human Tagged ORF Clone

CAT#: RC224012

OR5A2 (Myc-DDK-tagged)-Human olfactory receptor, family 5, subfamily A, member 2 (OR5A2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OR5A2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224012 representing NM_001001954
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTAGGAAGGAACAACACAATTGTGACAAAATTCATTCTCCTGGGACTTTCAGACCATCCTCAAA
  TGAAGATTTTCCTTTTCATGTTATTTCTGGGGCTCTACCTCCTGACGTTGGCCTGGAACTTAAGCCTCAT
  TGCCCTCATTAAGATGGACTCTCACCTGCACATGCCCATGTACTTCTTCCTCAGTAACCTGTCCTTCCTG
  GACATCTGCTATGTGTCCTCCACCGCCCCTAAGATGCTGTCTGACATCATCACAGAGCAGAAAACCATTT
  CCTTTGTTGGCTGTGCCACTCAGTACTTTGTCTTCTGTGGGATGGGGCTGACTGAATGCTTTCTCCTGGC
  AGCTATGGCCTATGACCGGTATGCTGCAATCTGCAACCCCTTGCTTTACACAGTCCTCATATCCCATACA
  CTTTGTTTAAAGATGGTGGTTGGCGCCTATGTGGGTGGATTCCTTAGTTCTTTCATTGAAACATACTCTG
  TCTATCAGCATGATTTCTGTGGGCCCTATATGATCAACCACTTTTTCTGTGACCTCCCTCCAGTCCTGGC
  TCTGTCCTGCTCTGATACCTTCACCAGCGAGGTGGTGACCTTCATAGTCAGTGTTGTCGTTGGAATAGTG
  TCTGTGCTAGTGGTCCTCATCTCTTATGGTTACATTGTTGCTGCTGTTGTGAAGATCAGCTCAGCTACAG
  GTAGGACAAAGGCCTTCAGCACTTGTGCCTCTCACCTGACTGCTGTGACCCTCTTCTATGGTTCTGGATT
  CTTCATGTACATGCGACCCAGTTCCAGCTACTCCCTAAACAGGGACAAGGTGGTGTCCATATTCTATGCC
  TTGGTGATCCCCGTGGTGAATCCCATCATCTACAGTTTTAGGAATAAGGAGATTAAAAATGCCATGAGGA
  AAGCCATGGAAAGGGACCCCGGGATTTCTCACGGTGGACCATTCATTTTTATGACCTTGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224012 representing NM_001001954
  Red=Cloning site Green=Tags(s)
  
  MAVGRNNTIVTKFILLGLSDHPQMKIFLFMLFLGLYLLTLAWNLSLIALIKMDSHLHMPMYFFLSNLSFL
  DICYVSSTAPKMLSDIITEQKTISFVGCATQYFVFCGMGLTECFLLAAMAYDRYAAICNPLLYTVLISHT
  LCLKMVVGAYVGGFLSSFIETYSVYQHDFCGPYMINHFFCDLPPVLALSCSDTFTSEVVTFIVSVVVGIV
  SVLVVLISYGYIVAAVVKISSATGRTKAFSTCASHLTAVTLFYGSGFFMYMRPSSSYSLNRDKVVSIFYA
  LVIPVVNPIIYSFRNKEIKNAMRKAMERDPGISHGGPFIFMTLG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001001954
• ORF Size: 972 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001954.1, NP_001001954.1
• RefSeq Size: 975 bp
• RefSeq ORF: 975 bp
• Locus ID: 219981
• Cytogenetics: 11q12.1
• Protein Families: Transmembrane
• Protein Pathways: Olfactory transduction
• MW: 35.8 kDa
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]