NNT (NM_182977) Human Tagged ORF Clone

CAT#: RC224048

NNT (Myc-DDK-tagged)-Human nicotinamide nucleotide transhydrogenase (NNT), nuclear gene encoding mitochondrial protein, transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY405292) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224048 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NNT
• Synonyms: GCCD4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224048 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAACCTATTGAAAACAGTGGTGACTGGCTGCTCGTGTCCTCTACTTAGCAATTTGGGGTCCTGTA
  AGGGTCTACGTGTGAAGAAGGATTTTTTACGAACATTTTATACTCACCAAGAACTGTGGTGTAAAGCGCC
  TGTAAAACCAGGAATTCCATATAAGCAACTGACTGTTGGAGTCCCCAAAGAGATATTCCAAAATGAGAAG
  CGAGTGGCATTGTCTCCTGCTGGTGTTCAGAACTTGGTCAAGCAGGGTTTTAATGTTGTCGTGGAATCGG
  GTGCGGGCGAAGCTTCCAAGTTCTCAGATGATCACTATAGAGTGGCAGGTGCCCAAATCCAAGGGGCAAA
  GGAAGTGCTGGCTTCTGATTTGGTGGTCAAAGTGCGAGCCCCTATGGTTAATCCAACATTAGGTGTTCAT
  GAAGCTGACCTTTTAAAGACATCAGGAACGCTGATTAGTTTTATTTACCCAGCCCAAAATCCAGAGTTGC
  TAAATAAACTTTCCCAAAGAAAAACTACAGTTCTGGCAATGGACCAGGTTCCAAGAGTCACAATTGCTCA
  GGGATATGATGCGCTAAGCTCCATGGCCAACATTGCGGGTTATAAGGCTGTTGTCCTAGCAGCAAATCAT
  TTTGGACGTTTTTTTACTGGTCAGATCACAGCTGCTGGAAAAGTTCCTCCAGCTAAGATTCTGATAGTTG
  GTGGTGGTGTTGCTGGGCTTGCTTCTGCAGGCGCAGCAAAGTCGATGGGTGCAATTGTTCGAGGATTTGA
  CACAAGAGCTGCAGCTTTGGAACAGTTCAAGTCTCTTGGTGCTGAGCCCTTGGAGGTGGACTTGAAGGAA
  TCTGGTGAGGGACAAGGAGGATATGCAAAAGAGATGTCCAAAGAGTTCATTGAAGCTGAAATGAAACTCT
  TTGCTCAACAATGCAAGGAGGTAGACATCCTTATCAGCACAGCACTTATTCCAGGTAAAAAAGCTCCAGT
  TTTATTTAATAAAGAAATGATTGAGTCAATGAAGGAAGGTTCAGTTGTTGTGGATTTAGCTGCTGAGGCT
  GGTGGAAACTTTGAAACCACTAAGCCAGGAGAACTCTACATTCATAAGGGAATTACTCACATAGGCTACA
  CAGACCTGCCCAGCCGAATGGCCACTCAGGCCAGCACCCTATATTCCAACAACATCACCAAACTCCTGAA
  GGCCATCAGCCCGGACAAAGATAATTTTTATTTTGATGTGAAAGATGACTTTGACTTTGGTACGATGGGT
  CATGTCATTAGAGGAACTGTAGTGATGAAAGATGGTAAAGTGATTTTCCCAGCTCCCACACCGAAAAATA
  TTCCTCAAGGTGCCCCAGTAAAACAGAAGACAGTGGCTGAGCTGGAAGCTGAAAAAGCAGCTACCATTAC
  ACCCTTCAGGAAGACAATGTCAACGGCTTCTGCATATACAGCAGGTCTCACAGGGATACTGGGTTTGGGC
  ATTGCGGCTCCCAATCTAGCCTTTTCTCAGATGGTGACCACTTTTGGCTTGGCTGGCATTGTGGGGTATC
  ATACCGTCTGGGGAGTGACCCCTGCTCTCCACTCACCACTGATGTCTGTGACAAATGCAATCTCAGGGCT
  GACTGCAGTTGGTGGGTTGGCACTGATGGGAGGACATTTGTATCCTTCCACAACTTCTCAGGGCCTTGCT
  GCTCTTGCTGCATTCATATCCTCTGTCAACATTGCAGGTGGCTTTCTGGTGACTCAGAGAATGCTGGACA
  TGTTCAAGCGTCCCACTGACCCCCCAGAATACAACTACCTGTACCTGCTCCCTGCCGGCACCTTTGTTGG
  TGGATATTTAGCTGCCCTCTACAGTGGTTATAACATTGAACAGATCATGTACCTAGGCTCGGGTTTGTGC
  TGTGTCGGTGCCTTGGCTGGCCTCTCCACCCAGGGAACAGCACGTCTTGGCAATGCACTGGGCATGATTG
  GGGTTGCTGGAGGACTGGCAGCCACCCTCGGAGTCCTAAAACCGGGCCCAGAATTACTAGCTCAGATGTC
  TGGAGCGATGGCTTTGGGTGGTACCATTGGATTGACAATTGCCAAACGCATCCAGATTTCTGATTTACCT
  CAATTAGTTGCTGCTTTTCACAGTTTAGTGGGTTTGGCAGCTGTACTTACTTGCATAGCTGAGTACATTA
  TAGAATATCCACATTTTGCTACGGATGCAGCAGCAAATCTCACCAAGATTGTGGCCTACCTCGGCACTTA
  CATTGGTGGCGTCACCTTTAGTGGGTCTCTCATTGCCTATGGAAAATTGCAGGGTCTCCTGAAATCTGCC
  CCTCTCCTACTGCCTGGAAGGCACTTACTCAATGCAGGCTTACTGGCTGCTAGTGTGGGCGGGATAATCC
  CATTCATGGTGGACCCAAGCTTTACTACTGGCATCACCTGTCTGGGTTCAGTGTCTGCTCTCTCTGCTGT
  CATGGGTGTGACTTTGACAGCTGCTATTGGGGGTGCTGACATGCCCGTCGTTATCACTGTGCTGAACAGC
  TACTCAGGCTGGGCCCTGTGTGCAGAGGGCTTCCTGCTCAACAACAATCTGCTGACCATCGTGGGTGCAC
  TCATAGGCTCGTCTGGTGCTATCCTGTCATACATCATGTGTGTGGCAATGAATCGCTCCCTGGCTAATGT
  GATTCTTGGAGGCTATGGCACCACTTCAACAGCTGGTGGAAAACCCATGGAAATTTCTGGCACACATACG
  GAAATCAACCTTGACAATGCAATTGACATGATTCGAGAAGCTAATAGCATTATTATTACACCAGGCTATG
  GTCTCTGTGCAGCCAAAGCTCAATACCCCATTGCTGATTTGGTAAAGATGCTCACTGAGCAAGGCAAAAA
  AGTCAGGTTTGGAATTCACCCAGTTGCAGGCCGAATGCCTGGTCAGCTTAATGTGCTGCTGGCTGAGGCT
  GGTGTGCCATATGACATTGTGTTGGAAATGGATGAGATCAACCATGATTTTCCAGATACTGATTTGGTCC
  TTGTAATTGGAGCTAATGACACTGTTAATTCAGCAGCTCAAGAAGATCCCAACTCTATTATTGCAGGCAT
  GCCAGTCCTTGAGGTCTGGAAATCAAAGCAGGTGATTGTTATGAAGAGGTCTTTGGGTGTTGGCTATGCT
  GCAGTGGACAATCCAATCTTCTACAAACCTAACACGGCCATGCTTCTAGGTGATGCCAAGAAAACATGTG
  ACGCGCTCCAGGCGAAAGTTAGAGAATCCTATCAGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224048 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MANLLKTVVTGCSCPLLSNLGSCKGLRVKKDFLRTFYTHQELWCKAPVKPGIPYKQLTVGVPKEIFQNEK
  RVALSPAGVQNLVKQGFNVVVESGAGEASKFSDDHYRVAGAQIQGAKEVLASDLVVKVRAPMVNPTLGVH
  EADLLKTSGTLISFIYPAQNPELLNKLSQRKTTVLAMDQVPRVTIAQGYDALSSMANIAGYKAVVLAANH
  FGRFFTGQITAAGKVPPAKILIVGGGVAGLASAGAAKSMGAIVRGFDTRAAALEQFKSLGAEPLEVDLKE
  SGEGQGGYAKEMSKEFIEAEMKLFAQQCKEVDILISTALIPGKKAPVLFNKEMIESMKEGSVVVDLAAEA
  GGNFETTKPGELYIHKGITHIGYTDLPSRMATQASTLYSNNITKLLKAISPDKDNFYFDVKDDFDFGTMG
  HVIRGTVVMKDGKVIFPAPTPKNIPQGAPVKQKTVAELEAEKAATITPFRKTMSTASAYTAGLTGILGLG
  IAAPNLAFSQMVTTFGLAGIVGYHTVWGVTPALHSPLMSVTNAISGLTAVGGLALMGGHLYPSTTSQGLA
  ALAAFISSVNIAGGFLVTQRMLDMFKRPTDPPEYNYLYLLPAGTFVGGYLAALYSGYNIEQIMYLGSGLC
  CVGALAGLSTQGTARLGNALGMIGVAGGLAATLGVLKPGPELLAQMSGAMALGGTIGLTIAKRIQISDLP
  QLVAAFHSLVGLAAVLTCIAEYIIEYPHFATDAAANLTKIVAYLGTYIGGVTFSGSLIAYGKLQGLLKSA
  PLLLPGRHLLNAGLLAASVGGIIPFMVDPSFTTGITCLGSVSALSAVMGVTLTAAIGGADMPVVITVLNS
  YSGWALCAEGFLLNNNLLTIVGALIGSSGAILSYIMCVAMNRSLANVILGGYGTTSTAGGKPMEISGTHT
  EINLDNAIDMIREANSIIITPGYGLCAAKAQYPIADLVKMLTEQGKKVRFGIHPVAGRMPGQLNVLLAEA
  GVPYDIVLEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSLGVGYA
  AVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182977
• ORF Size: 3258 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182977.1, NM_182977.2, NP_892022.1
• RefSeq Size: 4655 bp
• RefSeq ORF: 3261 bp
• Locus ID: 23530
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Nicotinate and nicotinamide metabolism
• MW: 113.9 kDa
• Gene Summary: This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. [provided by RefSeq, Sep 2016]