PCDHB6 (NM_018939) Human Tagged ORF Clone

CAT#: RC224130

PCDHB6 (Myc-DDK-tagged)-Human protocadherin beta 6 (PCDHB6)

Product Images

Western blot validation of overexpression lysate (Cat# LY402714) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224130 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCDHB6
• Synonyms: PCDH-BETA6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224130 representing NM_018939
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCAAACTAAAGTACAGAACAAGAAAAGGCAAGTGGCTTTCTTCATTTTATTGATGCTTTGGGGAG
  AGGTGGGTTCTGAATCGATTCAGTATTCCGTATTGGAGGAGACAGAAAGTGGCACGTTTGTGGCCAACTT
  GACAAAGGACCTGGGACTGAGGGTGGGGGAGCTGGCTTCGCGGGGCGCTCGGGTTGTTTTCAAAGGGAAC
  AGACAACATTTGCAGTTTGATCCACAGACCCATGATTTACTGCTAAATGAAAAACTGGACCGGGAGGAGC
  TGTGTGGCTCCACTGAGCCGTGTGTGCTACCTTTCCAAGTGTTACTGGAAAACCCCTTGCAGTTTTTTCA
  GGCTTCCTTGCGAGTCAGAGATATAAATGACCACGCCCCGGAATTCCCTGCCAGAGAAATGCTCCTGAAA
  ATATCAGAAATTACTATGCCAGGAAAGATATTTCCTTTGAAAATGGCACACGATTTAGACACCGGCAGCA
  ACGGCCTTCAGAGGTACACAATCAGCTCCAACCCTCACTTCCACGTTCTCACCCGCAATCGCAGCGAAGG
  CAGGAAGTTCCCGGAGCTGGTGCTAGACAAACCGTTGGACCGCGAGGAGCAGCCCCAACTCAGGCTAACG
  CTGATCGCGCTGGATGGCGGGTCTCCGCCCCGGTCAGGGACCTCCGAGATTCAGATCCAGATTTTGGACA
  TCAATGACAACGTCCCCGAGTTTGCTCAGGAGCTCTATGAAGCACAAGTCCCTGAGAACAACCCCCTCGG
  CTCTCTGGTTATTACCGTCTCAGCCAGAGATTTAGATGCAGGATCGTTTGGGAAGGTATCTTACGCCCTG
  TTTCAAGTCGATGACGTCAACCAACCCTTCGAAATAAACGCAATCACAGGAGAAATTCGGCTGAGAAAGG
  CTTTGGATTTTGAGGAAATTCAGTCTTATGACGTGGATGTTGAGGCTACAGATGGTGGAGGCCTATCAGG
  AAAATGCTCTTTAGTCGTCAGGGTCCTGGACGTGAATGACAATGCCCCTGAACTCACCATGTCGTTCTTC
  ATCAGCCTCATCCCAGAAAACTTACCAGAGATCACAGTGGCAGTTTTCAGTGTTTCAGATGCAGACTCTG
  GACATAACCAACAGGTTATTTGTTCAATAGAGAACAATCTCCCCTTTCTACTAAGACCTTCCGTGGAGAA
  TTTCTACACCCTGGTAACAGAAGGCGCGCTGGACAGAGAGAGCAGAGCCGAGTACAACATCACTATCACG
  GTCACTGATTTGGGGACACCAAGGCTGAAAACCCAGCAGAGCATAACTGTGCAGGTCTCCGACGTCAATG
  ACAACGCCCCCGCCTTCACCCAAACCTCCTACACCCTGTTCGTCCGCGAGAACAACAGCCCCGCCCTGCA
  CATCGGCAGCGTCAGCGCCACAGACAGAGACTCAGGCATCAACGCCCAGGTCACCTACTCGCTGCTGCCG
  CCCCAGGACCCGCACCTGCCCCTCTCTTCCCTGGTCTCCATCAACGCGGACAACGGCCACCTGTTTGCCC
  TCAGGTCGCTGGACTACGAGGCCCTGCAGTCTTTCGAGTTCCGCGTGGGCGCCACAGACCGCGGCTCCCC
  GGCGTTGAGCAGCGAGGCGCTGGTGCGCTTGCTGGTGCTGGACGCCAACGACAACTCGCCCTTCGTGTTG
  TACCCGCTGCAGAACGGCTCCGCGCCCTGCACCGAGCTGGTGCCCCGGGCGGCCGAGCCGGGCTACCTGG
  TGATCAAGGTGGTGGCGGTGGACGGCGACTCGGGCCAGAACGCCTGGCTGTCGTACCAGCTGCTCAAGGC
  CACGGAGCTCGGTCTGTTCGGCGTGTGGGCGCACAATGGCGAGGTGCGCACCGCCAGGCTGCTGAGCGAG
  CGAGACGCAGCCAAGCAGAGGCTGGTGGTGCTTGTCAAGGACAATGGCGAGCCTCCGCGCTCGGCCACCG
  CCACGCTGCACGTGCTCCTGGTGGACGGCTTCTCCCAGCCCTACCTGCCTCTCCCTGAGGCGGCCCCGGC
  CCAAGCCCAGGCCGACTCTCTCACCGTCTACCTGGTGGTGGCGTTGGCCTCGGTGTCGTCGCTCTTCCTC
  TTTTCGGTGCTCCTGTTCGTGGCGGTGCGGCTGTGCAGGAGGAGCAGGGCGGCCTCGGTGGGTCGCTACT
  CGGTGCCCGAGGGTCCCTTTCCAGGGCATCTGGTGGATGTGAGCGGCACCGGGACCCTATCCCAGAGCTA
  CCAGTACAAGGTGTGTCTGACGGGAGGCTCAGAAACAAATGAGTTCAAGTTCCTGAAGCCGATTATGCCC
  AACTTCCCTCCTCAGGGCACTGAGAGAGAAATGGAAGAAACCCCCACCTCTCGGAATAGCTTCCCGTTCA
  GT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224130 representing NM_018939
  Red=Cloning site Green=Tags(s)
  
  MMQTKVQNKKRQVAFFILLMLWGEVGSESIQYSVLEETESGTFVANLTKDLGLRVGELASRGARVVFKGN
  RQHLQFDPQTHDLLLNEKLDREELCGSTEPCVLPFQVLLENPLQFFQASLRVRDINDHAPEFPAREMLLK
  ISEITMPGKIFPLKMAHDLDTGSNGLQRYTISSNPHFHVLTRNRSEGRKFPELVLDKPLDREEQPQLRLT
  LIALDGGSPPRSGTSEIQIQILDINDNVPEFAQELYEAQVPENNPLGSLVITVSARDLDAGSFGKVSYAL
  FQVDDVNQPFEINAITGEIRLRKALDFEEIQSYDVDVEATDGGGLSGKCSLVVRVLDVNDNAPELTMSFF
  ISLIPENLPEITVAVFSVSDADSGHNQQVICSIENNLPFLLRPSVENFYTLVTEGALDRESRAEYNITIT
  VTDLGTPRLKTQQSITVQVSDVNDNAPAFTQTSYTLFVRENNSPALHIGSVSATDRDSGINAQVTYSLLP
  PQDPHLPLSSLVSINADNGHLFALRSLDYEALQSFEFRVGATDRGSPALSSEALVRLLVLDANDNSPFVL
  YPLQNGSAPCTELVPRAAEPGYLVIKVVAVDGDSGQNAWLSYQLLKATELGLFGVWAHNGEVRTARLLSE
  RDAAKQRLVVLVKDNGEPPRSATATLHVLLVDGFSQPYLPLPEAAPAQAQADSLTVYLVVALASVSSLFL
  FSVLLFVAVRLCRRSRAASVGRYSVPEGPFPGHLVDVSGTGTLSQSYQYKVCLTGGSETNEFKFLKPIMP
  NFPPQGTEREMEETPTSRNSFPFS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018939
• ORF Size: 2382 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018939.1, NM_018939.2, NM_018939.3, NP_061762.1
• RefSeq Size: 3030 bp
• RefSeq ORF: 2385 bp
• Locus ID: 56130
• Domains: CA
• Protein Families: Transmembrane
• MW: 84.1 kDa
• Gene Summary: This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. Unlike the alpha and gamma clusters, the transcripts from these genes do not share common 3' exons. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell neural connections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]