SOCS4 (NM_080867) Human Tagged ORF Clone

CAT#: RC224144

SOCS4 (Myc-DDK-tagged)-Human suppressor of cytokine signaling 4 (SOCS4), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SOCS4
• Synonyms: SOCS7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224144 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAAAATAATGAAAATATTAGTAAAAATGTAGATGTAAGGCCCAAAACTAGTCGGAGCAGAAGTG
  CCGACAGAAAAGACGGTTATGTGTGGAGTGGAAAGAAGTTATCTTGGTCAAAAAAGAGTGAGAGTTATTC
  AGATGCTGAGACAGTGAATGGTATAGAGAAAACCGAAGTGTCTTTAAGGAACCAAGAAAGGAAGCACAGC
  TGTTCATCCATTGAGTTGGACTTAGATCATTCCTGTGGGCATCGATTTTTAGGCCGATCTCTTAAACAGA
  AACTGCAAGATGCCGTGGGGCAGTGTTTTCCAATAAAGAATTGTAGTAGTCGGCACTCTTCAGGGCTTCC
  GTCTAAAAGGAAAATTCATATCAGTGAACTCATGTTAGATAAGTGTCCTTTCCCACCTCGATCAGATTTA
  GCCTTTAGGTGGCATTTTATTAAACGACACACTGCTCCTATAAATTCCAAATCAGATGAATGGGTAAGCA
  CAGACTTGTCTCAGACTGAATTGAGGGATGGTCAGCTAAAACGAAGAAATATGGAAGAAAATATAAACTG
  TTTCTCACATACCAATGTTCAGCCCTGTGTCATAACCACCGACAATGCTTTGTGTAGAGAAGGTCCTATG
  ACTGGCTCTGTGATGAACCTGGTTTCAAATAACAGTATAGAAGATAGTGATATGGATTCCGATGATGAAA
  TTCTAACACTTTGCACAAGTTCCAGAAAAAGAAACAAACCCAAATGGGATTTGGATGATGAAATCCTGCA
  GTTGGAAACACCTCCTAAATACCACACGCAGATTGATTATGTCCACTGTCTTGTACCAGACCTCCTTCAG
  ATCAATAACAACCCATGTTACTGGGGAGTGATGGATAAATACGCAGCCGAAGCACTACTGGAAGGAAAAC
  CAGAGGGTACCTTTTTACTTCGAGACTCAGCACAGGAAGACTATTTATTCTCTGTTAGTTTTAGACGCTA
  TAGTCGTTCTCTTCATGCTAGAATTGAACAGTGGAATCACAACTTTAGCTTTGATGCACATGACCCCTGT
  GTCTTCCATTCTCCTGACATTACTGGGCTCCTAGAACATTATAAGGACCCAAGCGCCTGTATGTTCTTTG
  AACCACTTCTATCCACTCCCTTAATTCGGACTTTCCCTTTTTCCCTGCAGCATATATGCAGAACAGTTAT
  TTGTAACTGTACAACTTATGATGGCATCGATGCCCTTCCAATTCCTTCTTCTATGAAATTATATCTGAAG
  GAATATCATTATAAATCAAAAGTTAGAGTACTCAGGATTGATGCACCAGAACAGCAATGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224144 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAENNENISKNVDVRPKTSRSRSADRKDGYVWSGKKLSWSKKSESYSDAETVNGIEKTEVSLRNQERKHS
  CSSIELDLDHSCGHRFLGRSLKQKLQDAVGQCFPIKNCSSRHSSGLPSKRKIHISELMLDKCPFPPRSDL
  AFRWHFIKRHTAPINSKSDEWVSTDLSQTELRDGQLKRRNMEENINCFSHTNVQPCVITTDNALCREGPM
  TGSVMNLVSNNSIEDSDMDSDDEILTLCTSSRKRNKPKWDLDDEILQLETPPKYHTQIDYVHCLVPDLLQ
  INNNPCYWGVMDKYAAEALLEGKPEGTFLLRDSAQEDYLFSVSFRRYSRSLHARIEQWNHNFSFDAHDPC
  VFHSPDITGLLEHYKDPSACMFFEPLLSTPLIRTFPFSLQHICRTVICNCTTYDGIDALPIPSSMKLYLK
  EYHYKSKVRVLRIDAPEQQC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080867
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080867.1, NM_080867.2, NP_543143.1
• RefSeq Size: 6900 bp
• RefSeq ORF: 1323 bp
• Locus ID: 122809
• Domains: SH2, SOCS
• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway, Jak-STAT signaling pathway, Type II diabetes mellitus
• MW: 50.6 kDa
• Gene Summary: The protein encoded by this gene contains a SH2 domain and a SOCS BOX domain. The protein thus belongs to the suppressor of cytokine signaling (SOCS), also known as STAT-induced STAT inhibitor (SSI), protein family. SOCS family members are known to be cytokine-inducible negative regulators of cytokine signaling. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]