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ACAT1 (ACACA) (NM_198836) Human Tagged ORF Clone
CAT#: RC224181
- TrueORF®
ACACA (Myc-DDK-tagged)-Human acetyl-CoA carboxylase alpha (ACACA), transcript variant 3
"NM_198836" in other vectors (2)
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Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Symbol | ACACA |
| Synonyms | ACAC; ACACAD; ACC; ACC1; ACCA |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224181 representing NM_198836
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAACCATCTCCCTTGGCCCAACCTCTGGAGCTGAACCAGCACTCTCGATTCATAATAGGTTCTG TGTCTGAAGATAACTCAGAGGATGAGATCAGCAACCTGGTGAAGTTGGACCTACTGGAGGAGAAGGAGGG CTCCTTGTCACCTGCTTCTGTTGGCTCAGATACACTCTCTGATTTGGGGATCTCTAGCCTACAGGATGGC TTGGCCTTGCACATAAGGTCCAGCATGTCTGGCTTGCACCTAGTAAAGCAGGGCCGAGACAGAAAGAAAA TAGATTCTCAACGAGATTTCACTGTGGCTTCTCCAGCAGAATTTGTTACTCGCTTTGGGGGAAATAAAGT GATTGAGAAGGTTCTTATTGCTAACAATGGCATTGCAGCAGTGAAATGCATGCGGTCTATCCGTAGGTGG TCTTATGAAATGTTTCGAAATGAACGTGCAATTAGATTCGTTGTCATGGTCACACCTGAAGACCTTAAAG CCAATGCAGAATACATTAAGATGGCAGATCACTATGTGCCAGTGCCTGGAGGACCAAACAACAACAACTA TGCAAATGTGGAATTAATTCTTGATATTGCTAAAAGGATCCCAGTACAAGCAGTGTGGGCTGGCTGGGGT CATGCTTCTGAGAATCCCAAACTACCGGAACTTCTCTTGAAAAATGGCATTGCCTTCATGGGTCCTCCAA GCCAGGCCATGTGGGCTTTAGGGGATAAGATTGCATCTTCCATAGTGGCTCAAACTGCAGGTATCCCAAC TCTTCCCTGGAGCGGCAGTGGTCTTCGTGTGGACTGGCAGGAAAATGATTTTTCAAAACGTATCTTAAAT GTTCCCCAGGAGCTATATGAAAAAGGTTATGTGAAAGATGTGGATGATGGGCTACAGGCAGCTGAGGAAG TTGGATATCCAGTAATGATCAAGGCCTCAGAGGGAGGAGGAGGGAAGGGAATTAGAAAAGTCAACAATGC AGATGACTTCCCTAATCTCTTCAGACAGGTTCAAGCTGAAGTTCCTGGATCTCCCATATTTGTGATGAGA CTAGCCAAACAATCTCGTCATCTGGAGGTGCAGATCTTAGCGGACCAATATGGCAATGCTATCTCTTTGT TTGGTCGTGATTGCTCTGTACAACGCAGGCATCAGAAGATTATTGAAGAAGCACCTGCTACTATTGCTAC TCCAGCAGTATTTGAACACATGGAACAGTGTGCGGTGAAACTTGCCAAAATGGTGGGTTATGTGAGTGCT GGGACTGTGGAATACCTGTACAGCCAGGATGGCAGCTTCTACTTTCTGGAATTGAATCCTCGGCTGCAGG TAGAGCACCCTTGTACAGAGATGGTGGCTGATGTCAATCTCCCTGCAGCACAGCTCCAGATTGCCATGGG GATTCCTCTATATAGAATCAAGGATATCCGTATGATGTATGGGGTATCTCCCTGGGGTGATTCTCCCATT GATTTTGAAGATTCTGCACACGTTCCTTGTCCAAGGGGCCATGTTATTGCTGCTCGGATCACTAGTGAAA ATCCAGATGAGGGTTTTAAGCCCAGCTCAGGAACAGTTCAGGAGCTAAATTTCCGCAGCAATAAGAATGT TTGGGGATATTTCAGTGTTGCTGCTGCAGGGGGACTTCATGAATTTGCTGATTCTCAGTTTGGTCACTGC TTTTCTTGGGGAGAAAACAGAGAAGAGGCAATTTCAAACATGGTGGTGGCTTTGAAGGAGCTGTCTATTC GGGGTGACTTTCGAACTACAGTTGAATACCTGATCAAATTGTTAGAGACTGAAAGCTTTCAGATGAACAG AATTGATACTGGCTGGCTGGACAGACTGATAGCAGAAAAAGTACAGGCTGAGCGACCTGACACCATGTTG GGGGTTGTGTGTGGTGCCCTCCACGTGGCAGATGTGAGCCTGCGGAATAGCGTCTCTAACTTCCTTCACT CCTTAGAAAGGGGTCAAGTCCTTCCTGCTCATACACTTCTGAATACAGTAGATGTTGAACTTATCTATGA GGGAGTCAAGTATGTACTTAAGGTGACTCGACAGTCCCCCAACTCCTATGTGGTGATCATGAATGGCTCA TGTGTAGAAGTAGATGTACATCGGCTGAGTGACGGTGGACTGCTCTTGTCCTATGATGGCAGCAGTTATA CTACGTATATGAAAGAGGAAGTGGATAGATATCGCATCACAATTGGCAATAAAACCTGTGTGTTTGAGAA GGAAAATGACCCATCGGTGATGCGCTCACCTTCTGCTGGGAAGTTAATCCAGTACATTGTAGAAGATGGA GGTCATGTGTTTGCCGGCCAGTGCTATGCTGAGATTGAGGTAATGAAGATGGTAATGACCTTAACAGCTG TGGAGTCTGGCTGTATCCATTACGTCAAGCGACCTGGAGCAGCTCTTGACCCTGGCTGTGTACTAGCCAA AATGCAACTGGACAACCCCAGCAAGGTTCAGCAGGCTGAACTTCACACAGGTAGTCTGCCACGGATCCAG AGCACGGCACTCAGAGGCGAGAAACTCCATCGAGTGTTCCATTATGTCCTGGATAATCTGGTCAATGTAA TGAATGGATACTGCCTTCCAGATCCTTTCTTTAGCAGCAAGGTAAAAGACTGGGTAGAGCGATTGATGAA AACCCTCAGAGATCCCTCCCTGCCTCTCCTAGAATTGCAAGATATTATGACCAGTGTGTCTGGCCGCATT CCCCCCAATGTGGAGAAGTCTATCAAGAAGGAAATGGCTCAGTATGCTAGCAACATCACATCAGTCCTCT GTCAGTTTCCCAGCCAGCAGATTGCAAACATCCTAGATAGCCATGCAGCTACATTGAACCGGAAATCTGA ACGGGAAGTCTTCTTTATGAATACTCAGAGCATTGTTCAGCTGGTACAGAGGTACCGAAGTGGCATCCGA GGCCACATGAAGGCTGTGGTGATGGATCTGCTCCGGCAGTACCTGCGAGTAGAGACACAATTCCAGAATG GTCACTATGACAAATGTGTATTCGCCCTCCGAGAAGAGAATAAAAGTGACATGAACACTGTACTGAACTA CATCTTCTCTCACGCTCAAGTCACCAAGAAGAATCTTCTGGTCACAATGCTTATTGATCAGTTGTGTGGC CGGGACCCTACTCTCACTGATGAGCTGCTGAATATTCTCACAGAGCTAACTCAACTCAGTAAGACCACCA ATGCCAAAGTAGCACTTCGAGCACGCCAGGTTCTTATTGCCTCCCATTTGCCATCATATGAGCTTCGCCA TAACCAAGTAGAGTCTATCTTCCTATCAGCTATTGACATGTATGGACATCAATTTTGCATTGAGAACCTG CAGAAACTCATCCTATCAGAAACATCTATTTTTGATGTCCTACCAAACTTCTTCTATCACAGCAACCAAG TAGTGAGGATGGCAGCTCTGGAGGTGTATGTTCGAAGGGCTTATATTGCCTATGAACTTAACAGCGTACA ACACCGCCAGCTTAAGGACAACACCTGTGTGGTGGAATTCCAGTTCATGCTGCCCACATCTCATCCAAAC AGAGGGAACATCCCTACGCTAAACAGAATGTCCTTCTCCTCCAACCTCAACCACTATGGCATGACCCATG TAGCTAGTGTCAGCGATGTACTGTTGGACAACTCATTCACTCCACCTTGTCAGCGGATGGGCGGAATGGT CTCTTTTCGGACTTTTGAAGATTTTGTCAGGATCTTTGATGAAGTGATGGGCTGCTTCTCTGACTCCCCA CCCCAGAGTCCCACATTCCCTGAGGCAGGTCACACGTCTCTTTATGATGAGGATAAGGTTCCCAGGGATG AACCAATTCACATTCTCAATGTGGCTATCAAGACTGACTGTGATATTGAGGATGACAGGCTGGCAGCTAT GTTCAGAGAATTTACCCAGCAAAATAAAGCTACCCTGGTTGACCATGGGATCCGGCGCCTTACTTTCCTG GTTGCACAAAAGGATTTCAGAAAGCAGGTCAACTATGAGGTGGATCGGAGATTTCATAGAGAATTCCCTA AATTTTTTACATTCCGAGCAAGGGATAAGTTTGAGGAGGATCGTATCTATCGTCATCTGGAGCCTGCTCT GGCTTTCCAGTTAGAGCTGAACCGGATGAGAAATTTTGACCTCACTGCCATTCCATGTGCTAATCACAAG ATGCACCTGTATCTCGGGGCAGCCAAGGTGGAAGTGGGCACAGAAGTGACAGACTACAGGTTCTTTGTTC GTGCAATCATCAGGCATTCTGATCTGGTCACCAAGGAAGCTTCTTTTGAATATCTGCAAAATGAAGGGGA GCGGCTACTCCTGGAAGCCATGGATGAGTTGGAAGTTGCTTTTAACAATACAAATGTCCGCACTGACTGT AACCACATCTTCCTCAACTTTGTGCCCACGGTTATCATGGACCCATCAAAGATTGAGGAATCCGTGCGGA GCATGGTAATGCGGTATGGAAGTCGCCTGTGGAAATTGCGCGTCCTCCAGGCAGAACTGAAAATCAACAT TCGCCTGACGCCAACTGGAAAAGCAATTCCCATCCGCCTCTTCCTGACAAACGAGTCTGGCTATTACTTG GATATCAGCCTATACAAGGAAGTGACTGACTCCAGGACAGCACAGATCATGTTTCAGGCATATGGAGACA AACAGGGACCACTGCATGGAATGTTAATCAATACTCCATATGTGACCAAAGACCTGCTGCAATCAAAGAG GTTCCAGGCACAATCCTTAGGGACAACATACATATATGATATCCCAGAGATGTTTCGGCAGTCCCTGATC AAACTCTGGGAGTCTATGTCCACTCAAGCATTTCTTCCATCTCCCCCTCTGCCTTCTGACATGCTGACTT ACACTGAACTGGTACTGGATGATCAAGGTCAGCTGGTCCACATGAACAGGCTTCCAGGAGGAAATGAGAT TGGCATGGTAGCTTGGAAAATGACCTTTAAAAGTCCTGAATATCCAGAAGGCCGAGATATCATTGTTATT GGCAATGACATCACATACCGAATTGGGTCCTTTGGGCCTCAAGAGGATTTGTTATTTCTCAGAGCTTCCG AACTTGCTAGGGCAGAAGGTATTCCACGCATCTATGTATCAGCCAACAGTGGAGCAAGAATCGGACTGGC AGAAGAAATTCGCCATATGTTTCATGTGGCCTGGGTAGATCCTGAGGATCCTTACAAGGGATACAGGTAT TTATATCTGACTCCTCAAGATTATAAGAGAGTCAGTGCTCTCAACTCTGTCCATTGTGAACACGTGGAAG ATGAAGGAGAATCCAGGTACAAGATAACAGATATTATTGGGAAAGAAGAGGGAATTGGACCCGAGAACCT TCGAGGTTCTGGAATGATTGCTGGAGAATCCTCATTGGCCTATAATGAGATCATTACCATCAGCCTGGTG ACGTGCCGGGCCATTGGGATTGGGGCTTACCTTGTCCGGCTGGGACAGAGAACCATCCAGGTTGAGAATT CTCACTTAATTCTAACAGGAGCTGGAGCCCTCAACAAAGTCCTCGGGCGGGAAGTGTACACCTCCAATAA CCAGCTGGGGGGCATCCAGATTATGCACAACAATGGGGTGACCCACTGCACTGTGTGTGATGACTTTGAA GGGGTTTTCACTGTCCTGCACTGGCTGTCTTACATGCCCAAGAGCGTGCACAGTTCAGTTCCTCTTCTGA ACTCAAAGGATCCTATAGACAGAATCATCGAGTTTGTTCCCACAAAGACCCCATACGATCCTCGATGGAT GCTAGCAGGCCGTCCTCACCCAACCCAAAAAGGTCAGTGGTTGAGTGGCTTTTTTGACTATGGATCTTTC TCAGAGATTATGCAGCCCTGGGCACAGACTGTGGTGGTTGGTAGAGCCAGGCTAGGAGGAATACCTGTGG GAGTTGTTGCTGTAGAAACCCGAACAGTAGAACTAAGTATCCCAGCTGATCCAGCAAACCTGGATTCTGA AGCCAAGATAATCCAGCAGGCTGGCCAGGTTTGGTTCCCAGATTCTGCGTTTAAGACGTATCAGGCCATC AAGGACTTCAACCGGGAAGGGCTGCCTCTGATGGTCTTTGCCAACTGGAGAGGCTTCTCTGGTGGAATGA AAGATATGTACGACCAAGTGCTGAAGTTTGGTGCTTACATTGTGGATGGCTTGAGGGAGTGCTGCCAGCC TGTGCTGGTTTACATTCCTCCCCAGGCTGAGCTGCGGGGTGGCTCCTGGGTGGTGATTGACTCCTCCATC AACCCCCGGCACATGGAGATGTATGCTGACCGAGAAAGCAGGGGATCTGTTCTGGAGCCAGAAGGGACAG TAGAAATCAAATTCCGCAGAAAGGATCTGGTGAAAACCATGCGTCGGGTGGACCCAGTCTACATCCACTT GGCTGAGCGATTGGGGACCCCAGAGCTAAGCACAGCTGAGCGGAAGGAGTTGGAGAACAAGTTGAAGGAG CGGGAGGAATTCCTAATTCCCATTTACCATCAGGTAGCCGTGCAGTTTGCTGACTTGCACGACACACCAG GCCGGATGCAGGAGAAGGGTGTTATTAGCGATATCCTGGATTGGAAAACATCCCGTACCTTCTTCTACTG GCGGCTGAGGCGTCTTCTGCTGGAGGACCTGGTCAAGAAGAAAATCCACAATGCCAACCCTGAGCTGACT GATGGCCAGATTCAAGCCATGTTAAGGCGCTGGTTTGTGGAAGTGGAAGGAACAGTGAAGGCTTATGTTT GGGACAATAATAAGGATCTGGCGGAGTGGCTAGAGAAACAGCTGACAGAGGAGGATGGTGTTCACTCGGT AATAGAGGAAAACATCAAATGCATCAGCAGAGACTACGTCCTCAAGCAAATCCGCAGCTTGGTCCAGGCC AATCCAGAGGTTGCCATGGATTCCATCATCCATATGACGCAGCACATATCACCCACTCAGCGAGCAGAAG TCATACGGATCCTCTCCACAATGGATTCCCCTTCCACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224181 representing NM_198836
Red=Cloning site Green=Tags(s) MDEPSPLAQPLELNQHSRFIIGSVSEDNSEDEISNLVKLDLLEEKEGSLSPASVGSDTLSDLGISSLQDG LALHIRSSMSGLHLVKQGRDRKKIDSQRDFTVASPAEFVTRFGGNKVIEKVLIANNGIAAVKCMRSIRRW SYEMFRNERAIRFVVMVTPEDLKANAEYIKMADHYVPVPGGPNNNNYANVELILDIAKRIPVQAVWAGWG HASENPKLPELLLKNGIAFMGPPSQAMWALGDKIASSIVAQTAGIPTLPWSGSGLRVDWQENDFSKRILN VPQELYEKGYVKDVDDGLQAAEEVGYPVMIKASEGGGGKGIRKVNNADDFPNLFRQVQAEVPGSPIFVMR LAKQSRHLEVQILADQYGNAISLFGRDCSVQRRHQKIIEEAPATIATPAVFEHMEQCAVKLAKMVGYVSA GTVEYLYSQDGSFYFLELNPRLQVEHPCTEMVADVNLPAAQLQIAMGIPLYRIKDIRMMYGVSPWGDSPI DFEDSAHVPCPRGHVIAARITSENPDEGFKPSSGTVQELNFRSNKNVWGYFSVAAAGGLHEFADSQFGHC FSWGENREEAISNMVVALKELSIRGDFRTTVEYLIKLLETESFQMNRIDTGWLDRLIAEKVQAERPDTML GVVCGALHVADVSLRNSVSNFLHSLERGQVLPAHTLLNTVDVELIYEGVKYVLKVTRQSPNSYVVIMNGS CVEVDVHRLSDGGLLLSYDGSSYTTYMKEEVDRYRITIGNKTCVFEKENDPSVMRSPSAGKLIQYIVEDG GHVFAGQCYAEIEVMKMVMTLTAVESGCIHYVKRPGAALDPGCVLAKMQLDNPSKVQQAELHTGSLPRIQ STALRGEKLHRVFHYVLDNLVNVMNGYCLPDPFFSSKVKDWVERLMKTLRDPSLPLLELQDIMTSVSGRI PPNVEKSIKKEMAQYASNITSVLCQFPSQQIANILDSHAATLNRKSEREVFFMNTQSIVQLVQRYRSGIR GHMKAVVMDLLRQYLRVETQFQNGHYDKCVFALREENKSDMNTVLNYIFSHAQVTKKNLLVTMLIDQLCG RDPTLTDELLNILTELTQLSKTTNAKVALRARQVLIASHLPSYELRHNQVESIFLSAIDMYGHQFCIENL QKLILSETSIFDVLPNFFYHSNQVVRMAALEVYVRRAYIAYELNSVQHRQLKDNTCVVEFQFMLPTSHPN RGNIPTLNRMSFSSNLNHYGMTHVASVSDVLLDNSFTPPCQRMGGMVSFRTFEDFVRIFDEVMGCFSDSP PQSPTFPEAGHTSLYDEDKVPRDEPIHILNVAIKTDCDIEDDRLAAMFREFTQQNKATLVDHGIRRLTFL VAQKDFRKQVNYEVDRRFHREFPKFFTFRARDKFEEDRIYRHLEPALAFQLELNRMRNFDLTAIPCANHK MHLYLGAAKVEVGTEVTDYRFFVRAIIRHSDLVTKEASFEYLQNEGERLLLEAMDELEVAFNNTNVRTDC NHIFLNFVPTVIMDPSKIEESVRSMVMRYGSRLWKLRVLQAELKINIRLTPTGKAIPIRLFLTNESGYYL DISLYKEVTDSRTAQIMFQAYGDKQGPLHGMLINTPYVTKDLLQSKRFQAQSLGTTYIYDIPEMFRQSLI KLWESMSTQAFLPSPPLPSDMLTYTELVLDDQGQLVHMNRLPGGNEIGMVAWKMTFKSPEYPEGRDIIVI GNDITYRIGSFGPQEDLLFLRASELARAEGIPRIYVSANSGARIGLAEEIRHMFHVAWVDPEDPYKGYRY LYLTPQDYKRVSALNSVHCEHVEDEGESRYKITDIIGKEEGIGPENLRGSGMIAGESSLAYNEIITISLV TCRAIGIGAYLVRLGQRTIQVENSHLILTGAGALNKVLGREVYTSNNQLGGIQIMHNNGVTHCTVCDDFE GVFTVLHWLSYMPKSVHSSVPLLNSKDPIDRIIEFVPTKTPYDPRWMLAGRPHPTQKGQWLSGFFDYGSF SEIMQPWAQTVVVGRARLGGIPVGVVAVETRTVELSIPADPANLDSEAKIIQQAGQVWFPDSAFKTYQAI KDFNREGLPLMVFANWRGFSGGMKDMYDQVLKFGAYIVDGLRECCQPVLVYIPPQAELRGGSWVVIDSSI NPRHMEMYADRESRGSVLEPEGTVEIKFRRKDLVKTMRRVDPVYIHLAERLGTPELSTAERKELENKLKE REEFLIPIYHQVAVQFADLHDTPGRMQEKGVISDILDWKTSRTFFYWRLRRLLLEDLVKKKIHNANPELT DGQIQAMLRRWFVEVEGTVKAYVWDNNKDLAEWLEKQLTEEDGVHSVIEENIKCISRDYVLKQIRSLVQA NPEVAMDSIIHMTQHISPTQRAEVIRILSTMDSPST TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_198836 |
| ORF Size | 7038 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_198836.1, NM_198836.2, NP_942133.1 |
| RefSeq Size | 9585 bp |
| RefSeq ORF | 7041 bp |
| Locus ID | 31 |
| Cytogenetics | 17q12 |
| Protein Families | Druggable Genome |
| Protein Pathways | Fatty acid biosynthesis, Insulin signaling pathway, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism |
| MW | 265.4 kDa |
| Gene Summary | 'Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]' |
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