AP4 (REPIN1) (NM_001099696) Human Tagged ORF Clone

CAT#: RC224200

REPIN1 (Myc-DDK-tagged)-Human replication initiator 1 (REPIN1), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY420504) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224200 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: REPIN1
• Synonyms: AP4; RIP60; Zfp464; ZNF464
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224200 representing NM_001099696
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGAACGTCGTTGCAGGGGCCCCCTGGCCATGGGCCTGGCCCAGCCCCGACTCCTTTCTGGGCCCT
  CCCAGGAGTCACCCCAGACCCTGGGGAAGGAGTCCCGCGGGCTGAGGCAACAAGGCACGTCAGTGGCCCA
  GTCTGGTGCCCAAGCCCCAGGCAGGGCCCATCGCTGTGCCCACTGTCGAAGGCACTTCCCTGGCTGGGTG
  GCTCTGTGGCTTCACACCCGCCGGTGCCAGGCCCGGCTGCCCTTGCCCTGCCCTGAGTGTGGCCGTCGCT
  TTCGCCATGCCCCCTTCTTAGCACTGCACCGCCAGGTCCATGCTGCTGCCACCCCAGACCTGGGCTTTGC
  CTGCCACCTCTGTGGGCAGAGCTTCCGAGGCTGGGTGGCCCTGGTTCTGCATCTGCGGGCCCATTCAGCT
  GCAAAGCGGCCCATCGCTTGTCCCAAATGCGAGAGACGCTTCTGGCGACGAAAGCAGCTTCGAGCTCATC
  TGCGGCGGTGCCACCCTCCCGCCCCGGAGGCCCGGCCCTTCATATGCGGCAACTGTGGCCGGAGCTTTGC
  CCAGTGGGACCAGCTAGTTGCCCACAAGCGGGTGCACGTAGCTGAGGCCCTGGAGGAGGCCGCAGCCAAG
  GCTCTGGGGCCCCGGCCCAGGGGCCGCCCCGCGGTGACCGCCCCCCGGCCCGGTGGAGATGCCGTCGACC
  GCCCCTTCCAGTGTGCCTGTTGTGGCAAGCGCTTCCGGCACAAGCCCAACTTGATCGCTCACCGCCGCGT
  GCACACGGGCGAGCGGCCCCACCAGTGCCCCGAGTGCGGGAAGCGCTTTACCAATAAGCCCTATCTGACT
  TCGCACCGGCGCATCCACACCGGCGAGAAGCCCTACCCGTGCAAAGAGTGCGGCCGCCGCTTCCGGCACA
  AACCCAACCTGCTGTCTCACAGCAAGATTCACAAGCGATCCGAGGGGTCGGCCCAGGCCGCCCCCGGCCC
  GGGGAGCCCCCAGCTGCCAGCCGGCCCCCAGGAGTCCGCGGCCGAGCCCACCCCGGCGGTACCTCTGAAA
  CCGGCCCAGGAGCCGCCGCCAGGGGCCCCGCCAGAGCACCCGCAGGACCCGATCGAAGCCCCCCCCTCCC
  TCTACAGCTGCGACGACTGCGGCAGGAGCTTCCGGCTGGAGCGCTTCCTGCGGGCCCACCAGCGGCAGCA
  CACCGGGGAGCGGCCCTTCACCTGCGCCGAGTGCGGGAAGAACTTCGGCAAGAAGACGCACCTGGTGGCG
  CACTCGCGCGTGCACTCCGGCGAGCGGCCCTTCGCCTGCGAGGAGTGCGGCCGCCGCTTCTCCCAGGGCA
  GCCATCTGGCGGCGCATCGGCGCGACCACGCCCCCGATCGGCCCTTCGTGTGTCCCGACTGCGGCAAGGC
  CTTCCGCCACAAACCCTACCTGGCGGCGCACCGGCGCATCCACACCGGCGAGAAGCCCTACGTCTGCCCC
  GACTGCGGCAAAGCCTTCAGCCAGAAGTCCAACCTGGTGTCGCACCGGCGCATCCACACGGGCGAGCGGC
  CCTACGCCTGTCCCGACTGCGACCGCAGCTTCAGCCAGAAGTCCAACCTCATCACCCACCGCAAGAGCCA
  CATCCGGGACGGCGCCTTCTGCTGTGCCATCTGTGGCCAGACCTTCGACGACGAGGAGAGACTCCTGGCC
  CACCAGAAGAAGCACGATGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224200 representing NM_001099696
  Red=Cloning site Green=Tags(s)
  
  MLERRCRGPLAMGLAQPRLLSGPSQESPQTLGKESRGLRQQGTSVAQSGAQAPGRAHRCAHCRRHFPGWV
  ALWLHTRRCQARLPLPCPECGRRFRHAPFLALHRQVHAAATPDLGFACHLCGQSFRGWVALVLHLRAHSA
  AKRPIACPKCERRFWRRKQLRAHLRRCHPPAPEARPFICGNCGRSFAQWDQLVAHKRVHVAEALEEAAAK
  ALGPRPRGRPAVTAPRPGGDAVDRPFQCACCGKRFRHKPNLIAHRRVHTGERPHQCPECGKRFTNKPYLT
  SHRRIHTGEKPYPCKECGRRFRHKPNLLSHSKIHKRSEGSAQAAPGPGSPQLPAGPQESAAEPTPAVPLK
  PAQEPPPGAPPEHPQDPIEAPPSLYSCDDCGRSFRLERFLRAHQRQHTGERPFTCAECGKNFGKKTHLVA
  HSRVHSGERPFACEECGRRFSQGSHLAAHRRDHAPDRPFVCPDCGKAFRHKPYLAAHRRIHTGEKPYVCP
  DCGKAFSQKSNLVSHRRIHTGERPYACPDCDRSFSQKSNLITHRKSHIRDGAFCCAICGQTFDDEERLLA
  HQKKHDV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001099696
• ORF Size: 1701 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001099696.1, NM_001099696.2, NP_001093166.1
• RefSeq Size: 2986 bp
• RefSeq ORF: 1704 bp
• Locus ID: 29803
• MW: 63.4 kDa
• Gene Summary: Sequence-specific double-stranded DNA-binding protein required for initiation of chromosomal DNA replication. Binds on 5'-ATT-3' reiterated sequences downstream of the origin of bidirectional replication (OBR) and a second, homologous ATT sequence of opposite orientation situated within the OBR zone. Facilitates DNA bending. [UniProtKB/Swiss-Prot Function]