MPRIP (NM_201274) Human Tagged ORF Clone

CAT#: RC224291

MPRIP (Myc-DDK-tagged)-Human myosin phosphatase Rho interacting protein (MPRIP), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY404508) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224291 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MPRIP
• Synonyms: M-RIP; MRIP; p116Rip; RHOIP3; RIP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224291 representing NM_201274
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGCAGCCAAGGAGAACCCGTGCAGGAAATTCCAGGCCAACATCTTCAACAAGAGCAAGTGTCAGA
  ACTGCTTCAAGCCCCGCGAGTCGCATCTGCTCAACGACGAGGACCTGACGCAGGCAAAACCCATTTATGG
  CGGTTGGCTGCTCCTGGCTCCAGATGGGACCGACTTTGACAACCCAGTGCACCGGTCTCGGAAATGGCAG
  CGACGGTTCTTCATCCTTTACGAGCACGGCCTCTTGCGCTACGCCCTGGATGAGATGCCCACGACCCTTC
  CTCAGGGCACCATCAACATGAACCAGTGCACAGATGTGGTGGATGGGGAGGGCCGCACGGGCCAGAAGTT
  CTCCCTGTGTATTCTGACGCCTGAGAAGGAGCATTTCATCCGGGCGGAGACCAAGGAGATCGTCAGTGGG
  TGGCTGGAGATGCTCATGGTCTATCCCCGGACCAACAAGCAGAATCAGAAGAAGAAACGGAAAGTGGAGC
  CCCCCACACCACAGGAGCCTGGGCCTGCCAAGGTGGCTGTTACCAGCAGCAGCAGCAGCAGCAGCAGCAG
  CAGCAGCATCCCCAGTGCTGAGAAAGTCCCCACCACCAAGTCCACACTCTGGCAGGAAGAAATGAGGACC
  AAGGACCAGCCAGATGGCAGCAGCCTGAGTCCAGCTCAGAGTCCCAGCCAGAGCCAGCCTCCTGCTGCCA
  GCTCCCTGCGGGAACCTGGGCTAGAGAGCAAAGAAGAGGAGAGCGCCATGAGTAGCGACCGCATGGACTG
  TGGCCGCAAAGTCCGGGTGGAGAGCGGCTACTTCTCTCTGGAGAAGACCAAACAGGACTTGAAGGCTGAA
  GAACAGCAGCTGCCCCCGCCGCTCTCCCCTCCCAGCCCCAGCACCCCCAACCACAGGAGGTCCCAGGTGA
  TTGAAAAGTTTGAGGCCTTGGACATTGAGAAGGCAGAGCACATGGAGACCAATGCAGTGGGGCCCTCACC
  ATCCAGCGACACACGCCAGGGCCGCAGCGAGAAGAGGGCGTTCCCTAGGAAGCGGGACTTCACCAATGAA
  GCCCCCCCAGCTCCTCTCCCAGACGCCTCGGCTTCCCCCCTGTCTCCACACCGAAGAGCCAAGTCACTGG
  ACAGGAGGTCCACGGAGCCCTCCGTGACGCCCGACCTGCTGAATTTCAAGAAAGGCTGGCTGACTAAGCA
  GTATGAGGACGGCCAGTGGAAGAAACACTGGTTTGTCCTCGCCGATCAAAGCCTGAGATACTACAGGGAT
  TCAGTGGCTGAGGAGGCAGCCGACTTGGATGGAGAAATTGACTTGTCCGCATGTTACGATGTCACAGAGT
  ATCCAGTTCAGAGAAACTATGGCTTCCAGATACATACAAAGGAGGGCGAGTTTACCCTGTCGGCCATGAC
  ATCTGGGATTCGGCGGAACTGGATCCAGACCATCATGAAGCACGTGCACCCGACCACTGCCCCGGATGTG
  ACCAGCTCGTTGCCAGAGGAAAAAAACAAGAGCAGCTGCTCTTTTGAGACCTGCCCGAGGCCTACTGAGA
  AGCAAGAGGCAGAGCTGGGGGAGCCGGACCCTGAGCAGAAGAGGAGCCGCGCACGGGAGCGGAGGCGAGA
  GGGCCGCTCCAAGACCTTTGACTGGGCTGAGTTCCGTCCCATCCAGCAGGCCCTGGCTCAGGAGCGGGTG
  GGCGGCGTGGGGCCTGCTGACACCCACGAGCCCCTGCGCCCTGAGGCGGAGCCTGGGGAGCTGGAGCGGG
  AGCGTGCACGGAGGCGGGAGGAGCGCCGCAAGCGCTTCGGGATGCTCGACGCCACAGACGGGCCAGGCAC
  TGAGGATGCAGCCCTGCGCATGGAGGTGGACCGGAGCCCAGGGCTGCCTATGAGCGACCTCAAAACGCAT
  AACGTCCACGTGGAGATTGAGCAGCGGTGGCATCAGGTGGAGACCACACCTCTCCGGGAAGAGAAGCAGG
  TGCCCATCGCCCCCGTCCACCTGTCTTCTGAAGATGGGGGTGACCGGCTCTCCACACACGAGCTGACCTC
  TCTGCTCGAGAAGGAGCTGGAGCAGAGCCAGAAGGAGGCCTCAGACCTTCTGGAGCAGAACCGGCTCCTG
  CAGGACCAGCTGAGGGTGGCCCTGGGCCGGGAGCAGAGCGCCCGTGAGGGCTACGTGCTGCAGGCCACGT
  GCGAGCGAGGGTTTGCAGCAATGGAAGAAACGCACCAGAAGAAGATTGAAGATCTCCAGAGGCAGCACCA
  GCGGGAGCTAGAGAAACTTCGAGAAGAGAAAGACCGCCTCCTAGCCGAGGAGACAGCGGCCACCATCTCA
  GCCATCGAAGCCATGAAGAACGCCCACCGGGAGGAAATGGAGCGGGAGCTGGAGAAGAGCCAGCGGTCCC
  AGATCAGCAGCGTCAACTCGGATGTTGAGGCCCTGCGGCGCCAGTACCTGGAGGAGCTGCAGTCGGTGCA
  GCGGGAACTGGAGGTCCTCTCGGAGCAGTACTCGCAGAAGTGCCTGGAGAATGCCCATCTGGCCCAGGCG
  CTGGAGGCCGAGCGGCAGGCCCTGCGGCAGTGCCAGCGTGAGAACCAGGAGCTCAATGCCCACAACCAGG
  AGCTGAACAACCGCCTGGCTGCAGAGATCACACGGTTGCGGACGCTGCTGACTGGGGACGGCGGTGGGGA
  GGCCACTGGGTCACCCCTTGCACAGGGCAAGGATGCCTATGAACTAGAGGTCTTATTGCGGGTAAAGGAA
  TCGGAAATACAGTACCTGAAACAGGAGATTAGCTCCCTCAAGGATGAGCTGCAGACGGCACTGCGGGACA
  AGAAGTACGCAAGTGACAAGTACAAAGACATCTACACAGAGCTCAGCATCGCGAAGGCTAAGGCTGACTG
  TGACATCAGCAGGTTGAAGGAGCAGCTCAAGGCTGCAACGGAAGCACTGGGGGAGAAGTCCCCTGACAGT
  GCCACGGTGTCCGGATATGATATAATGAAATCTAAAAGCAACCCTGACTTCTTGAAGAAAGACAGATCCT
  GTGTCACCCGGCAACTCAGAAACATCAGGTCCAAGTCCGTAATTGAGCAGGTCTCGTGGGATACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224291 representing NM_201274
  Red=Cloning site Green=Tags(s)
  
  MSAAKENPCRKFQANIFNKSKCQNCFKPRESHLLNDEDLTQAKPIYGGWLLLAPDGTDFDNPVHRSRKWQ
  RRFFILYEHGLLRYALDEMPTTLPQGTINMNQCTDVVDGEGRTGQKFSLCILTPEKEHFIRAETKEIVSG
  WLEMLMVYPRTNKQNQKKKRKVEPPTPQEPGPAKVAVTSSSSSSSSSSSIPSAEKVPTTKSTLWQEEMRT
  KDQPDGSSLSPAQSPSQSQPPAASSLREPGLESKEEESAMSSDRMDCGRKVRVESGYFSLEKTKQDLKAE
  EQQLPPPLSPPSPSTPNHRRSQVIEKFEALDIEKAEHMETNAVGPSPSSDTRQGRSEKRAFPRKRDFTNE
  APPAPLPDASASPLSPHRRAKSLDRRSTEPSVTPDLLNFKKGWLTKQYEDGQWKKHWFVLADQSLRYYRD
  SVAEEAADLDGEIDLSACYDVTEYPVQRNYGFQIHTKEGEFTLSAMTSGIRRNWIQTIMKHVHPTTAPDV
  TSSLPEEKNKSSCSFETCPRPTEKQEAELGEPDPEQKRSRARERRREGRSKTFDWAEFRPIQQALAQERV
  GGVGPADTHEPLRPEAEPGELERERARRREERRKRFGMLDATDGPGTEDAALRMEVDRSPGLPMSDLKTH
  NVHVEIEQRWHQVETTPLREEKQVPIAPVHLSSEDGGDRLSTHELTSLLEKELEQSQKEASDLLEQNRLL
  QDQLRVALGREQSAREGYVLQATCERGFAAMEETHQKKIEDLQRQHQRELEKLREEKDRLLAEETAATIS
  AIEAMKNAHREEMERELEKSQRSQISSVNSDVEALRRQYLEELQSVQRELEVLSEQYSQKCLENAHLAQA
  LEAERQALRQCQRENQELNAHNQELNNRLAAEITRLRTLLTGDGGGEATGSPLAQGKDAYELEVLLRVKE
  SEIQYLKQEISSLKDELQTALRDKKYASDKYKDIYTELSIAKAKADCDISRLKEQLKAATEALGEKSPDS
  ATVSGYDIMKSKSNPDFLKKDRSCVTRQLRNIRSKSVIEQVSWDT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_201274
• ORF Size: 3075 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_201274.1, NM_201274.2, NM_201274.3, NP_958431.2
• RefSeq Size: 11023
• RefSeq ORF: 3078
• Locus ID: 23164
• Protein Families: Druggable Genome
• MW: 116.5 kDa