STEAP3 (NM_182915) Human Tagged ORF Clone

CAT#: RC224323

STEAP3 (Myc-DDK-tagged)-Human STEAP family member 3 (STEAP3), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY405328) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224323 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STEAP3
• Synonyms: AHMIO2; dudlin-2; dudulin-2; pHyde; STMP3; TSAP6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224323 representing NM_182915
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCACCAGCCTGCTGTTGCCACCAAAATGCCAGAAGAGATGGACAAGCCACTGATCAGCCTCCACC
  TGGTGGACAGCGATAGTAGCCTTGCCAAGGTCCCCGATGAGGCCCCCAAAGTGGGCATCCTGGGTAGCGG
  GGACTTTGCCCGCTCCCTGGCCACACGCCTGGTGGGCTCTGGCTTCAAAGTGGTGGTGGGGAGCCGCAAC
  CCCAAACGCACAGCCAGGCTGTTTCCCTCAGCGGCCCAAGTGACTTTCCAAGAGGAGGCAGTGAGCTCCC
  CGGAGGTCATCTTTGTGGCTGTGTTCCGGGAGCACTACTCTTCACTGTGCAGTCTCAGTGACCAGCTGGC
  GGGCAAGATCCTGGTGGATGTGAGCAACCCTACAGAGCAAGAGCACCTTCAGCATCGTGAGTCCAATGCT
  GAGTACCTGGCCTCCCTCTTCCCCACTTGCACAGTGGTCAAGGCCTTCAATGTCATCTCTGCCTGGACCC
  TGCAGGCTGGCCCAAGGGATGGTAACAGGCAGGTGCCCATCTGCGGTGACCAGCCAGAAGCCAAGCGTGC
  TGTCTCGGAGATGGCGCTCGCCATGGGCTTCATGCCCGTGGACATGGGATCCCTGGCGTCAGCCTGGGAG
  GTGGAGGCCATGCCCCTGCGCCTCCTCCCGGCCTGGAAGGTGCCCACCCTGCTGGCCCTGGGGCTCTTCG
  TCTGCTTCTATGCCTACAACTTCGTCCGGGACGTTCTGCAGCCCTATGTGCAGGAAAGCCAGAACAAGTT
  CTTCAAGCTGCCCGTGTCCGTGGTCAACACCACACTGCCGTGCGTGGCCTACGTGCTGCTGTCACTCGTG
  TACTTGCCCGGCGTGCTGGCGGCTGCCCTGCAGCTGCGGCGCGGCACCAAGTACCAGCGCTTCCCCGACT
  GGCTGGACCACTGGCTACAGCACCGCAAGCAGATCGGGCTGCTCAGCTTCTTCTGCGCCGCCCTGCACGC
  CCTCTACAGCTTCTGCTTGCCGCTGCGCCGCGCCCACCGCTACGACCTGGTCAACCTGGCAGTCAAGCAG
  GTCTTGGCCAACAAGAGCCACCTCTGGGTGGAGGAGGAGGTCTGGCGGATGGAGATCTACCTCTCCCTGG
  GAGTGCTGGCCCTCGGCACGTTGTCCCTGCTGGCCGTGACCTCACTGCCGTCCATTGCAAACTCGCTCAA
  CTGGAGGGAGTTCAGCTTCGTTCAGTCCTCACTGGGCTTTGTGGCCCTCGTGCTGAGCACACTGCACACG
  CTCACCTACGGCTGGACCCGCGCCTTCGAGGAGAGCCGCTACAAGTTCTACCTGCCTCCCACCTTCACGC
  TCACGCTGCTGGTGCCCTGCGTCGTCATCCTGGCCAAAGCCCTGTTTCTCCTGCCCTGCATCAGCCGCAG
  ACTCGCCAGGATCCGGAGAGGCTGGGAGAGGGAGAGCACCATCAAGTTCACGCTGCCCACAGACCACGCC
  CTGGCCGAGAAGACGAGCCACGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224323 representing NM_182915
  Red=Cloning site Green=Tags(s)
  
  MSHQPAVATKMPEEMDKPLISLHLVDSDSSLAKVPDEAPKVGILGSGDFARSLATRLVGSGFKVVVGSRN
  PKRTARLFPSAAQVTFQEEAVSSPEVIFVAVFREHYSSLCSLSDQLAGKILVDVSNPTEQEHLQHRESNA
  EYLASLFPTCTVVKAFNVISAWTLQAGPRDGNRQVPICGDQPEAKRAVSEMALAMGFMPVDMGSLASAWE
  VEAMPLRLLPAWKVPTLLALGLFVCFYAYNFVRDVLQPYVQESQNKFFKLPVSVVNTTLPCVAYVLLSLV
  YLPGVLAAALQLRRGTKYQRFPDWLDHWLQHRKQIGLLSFFCAALHALYSFCLPLRRAHRYDLVNLAVKQ
  VLANKSHLWVEEEVWRMEIYLSLGVLALGTLSLLAVTSLPSIANSLNWREFSFVQSSLGFVALVLSTLHT
  LTYGWTRAFEESRYKFYLPPTFTLTLLVPCVVILAKALFLLPCISRRLARIRRGWERESTIKFTLPTDHA
  LAEKTSHV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182915
• ORF Size: 1494 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_182915.1, NM_182915.2, NP_878919.2
• RefSeq Size: 4285
• RefSeq ORF: 1497
• Locus ID: 55240
• Protein Families: Transmembrane
• Protein Pathways: p53 signaling pathway
• MW: 55.5 kDa
• Gene Summary: This gene encodes a multipass membrane protein that functions as an iron transporter. The encoded protein can reduce both iron (Fe3+) and copper (Cu2+) cations. This protein may mediate downstream responses to p53, including promoting apoptosis. Deficiency in this gene can cause anemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]