Caspase 10 (CASP10) (NM_032974) Human Tagged ORF Clone
CAT#: RC224500L4
- LentiORF®
Lenti-ORF clone of CASP10 (mGFP-tagged)-Human caspase 10, apoptosis-related cysteine peptidase (CASP10), transcript variant 2
"NM_032974" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | CASP10 |
Synonyms | ALPS2; FLICE2; MCH4 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC224500).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_032974 |
ORF Size | 1563 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_032974.2, NP_116756.2 |
RefSeq Size | 2054 bp |
RefSeq ORF | 1566 bp |
Locus ID | 843 |
Cytogenetics | 2q33.1 |
Protein Families | Druggable Genome, Protease |
Protein Pathways | Apoptosis, RIG-I-like receptor signaling pathway |
MW | 58.8 kDa |
Gene Summary | 'This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 3 and 7, and the protein itself is processed by caspase 8. Mutations in this gene are associated with type IIA autoimmune lymphoproliferative syndrome, non-Hodgkin lymphoma and gastric cancer. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC313837 | CASP10 (untagged)-Human caspase 10, apoptosis-related cysteine peptidase (CASP10), transcript variant 2 |
USD 880.00 |
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RC224500 | CASP10 (Myc-DDK-tagged)-Human caspase 10, apoptosis-related cysteine peptidase (CASP10), transcript variant 2 |
USD 470.00 |
|
RG224500 | CASP10 (GFP-tagged) - Human caspase 10, apoptosis-related cysteine peptidase (CASP10), transcript variant 2 |
USD 520.00 |
|
RC224500L3 | Lenti-ORF clone of CASP10 (Myc-DDK-tagged)-Human caspase 10, apoptosis-related cysteine peptidase (CASP10), transcript variant 2 |
USD 670.00 |
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