CUX1 (NM_181552) Human Tagged ORF Clone

CAT#: RC224512

CUX1 (Myc-DDK-tagged)-Human cut-like homeobox 1 (CUX1), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CUX1
• Synonyms: CASP; CDP; CDP/Cut; CDP1; Clox; COY1; CUTL1; CUX; Cux/CDP; GDDI; GOLIM6; Nbla10317; p75; p100; p110; p200
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224512 representing NM_181552
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGTGCGTAGCCGGAGCCAGGTTGAAGAGAGAACTCGATGCCACCGCAACGGTATTGGCGAACCGGC
  AGGATGAAAGTGAGCAGTCCAGAAAGCGGCTTATCGAACAGAGCCGGGAGTTCAAGAAGAACACTCCAGA
  GGATTTGCGCAAGCAGGTAGCGCCGCTGCTGAAGAGTTTCCAAGGAGAGATTGATGCACTGAGTAAAAGA
  AGCAAGGAAGCTGAAGCAGCTTTCTTGAATGTCTACAAAAGATTGATTGACGTCCCAGATCCCGTACCAG
  CTTTGGATCTCGGACAGCAACTCCAGCTCAAAGTGCAGCGCCTGCACGATATTGAAACAGAGAACCAGAA
  ACTTAGGGAAACTCTGGAAGAATACAACAAGGAATTTGCTGAAGTGAAAAATCAAGAGGTTACGATAAAA
  GCACTTAAAGAGAAAATCCGAGAATATGAACAGACACTGAAGAACCAAGCCGAAACCATAGCTCTTGAGA
  AGGAACAGAAGTTACAGAATGACTTTGCAGAAAAGGAGAGAAAGCTGCAGGAGACACAGATGTCCACCAC
  CTCAAAGCTGGAGGAAGCTGAGCATAAGGTTCAGAGCCTACAAACAGCCCTGGAAAAAACTCGAACAGAA
  TTATTTGACCTGAAAACCAAATACGATGAAGAAACTACTGCAAAGGCCGACGAGATTGAAATGATCATGA
  CGGACCTTGAAAGGGCAAACCAGAGGGCAGAGGTGGCTCAGAGAGAGGCGGAGACCTTAAGGGAACAGCT
  CTCATCGGCCAATCACTCCCTCCAGCTGGCCTCACAGATCCAGAAGGCACCAGACGTGGAGCAGGCCATA
  GAGGTGCTGACCCGCTCCAGCCTAGAAGTTGAGTTGGCCGCCAAGGAGCGGGAGATCGCACAGCTGGTGG
  AGGACGTGCAGAGACTCCAGGCCAGCCTCACCAAGCTGCGGGAGAATTCGGCCAGCCAGATCTCACAGCT
  TGAGCAGCAGCTGAGCGCCAAAAACAGCACACTCAAACAACTGGAAGAAAAACTCAAAGGCCAGGCTGAC
  TATGAAGAGGTGAAGAAAGAGCTGAACATTCTGAAGTCCATGGAGTTTGCACCGTCCGAGGGCGCTGGGA
  CACAGGATGCGGCCAAGCCCCTGGAGGTGCTGTTGCTGGAGAAGAACCGCTCGCTGCAGTCCGAGAACGC
  CGCGCTGCGCATCTCCAACAGCGACCTGAGCGGGTCAGCCAGGAGGAAAGGGAAAGACCAGCCTGAAAGT
  CGGCGCCCGGGATCTTTGCCGGCCCCCCCTCCTTCTCAGTTGCCCCGCAACCCGGGGGAGCAGGCTTCCA
  ATACTAATGGTACACACCAGTTCTCACCAGCGGGGTTAAGTCAAGACTTTTTCAGCTCATCCCTGGCAAG
  CCCCAGCCTACCCCTGGCTTCTACAGGAAAATTTGCACTAAACTCTCTTCTCCAGCGGCAGCTAATGCAG
  TCCTTCTACTCCAAGGCTATGCAGGAAGCCGGAAGCACAAGCATGATTTTTTCAACAGGTCCATACAGCA
  CAAACTCCATATCTTCCCAAAGTCCATTACAACAAAGCCCAGATGTCAATGGCATGGCCCCATCCCCCAG
  CCAGTCAGAAAGTGCTGGGAGCGTCTCCGAGGGCGAGGAGATGGACACTGCAGAAATCGCCCGGCAGGTC
  AAAGAGCAGCTGATTAAGCACAATATCGGACAACGTATTTTCGGACATTATGTGTTGGGACTGTCTCAAG
  GGTCCGTGAGCGAGATTCTGGCCCGGCCCAAGCCATGGAATAAACTGACTGTTCGTGGCAAGGAGCCATT
  TCACAAGATGAAACAGTTCCTCTCCGATGAGCAGAACATCCTGGCCCTCCGTAGCATCCAAGGCAGACAA
  AGAGAGAATCCAGGCCAGAGCCTGAACAGACTATTTCAGGAAGTACCGAAACGAAGAAATGGGTCTGAAG
  GTAACATCACCACCCGGATCCGAGCCTCGGAGACTGGCTCTGATGAAGCCATCAAGTCCATCCTAGAGCA
  AGCCAAGAGGGAGCTCCAAGTGCAGAAAACTGCAGAGCCGGCCCAGCCTTCCTCCGCATCCGGCAGCGGG
  AACTCTGATGACGCCATCCGCTCCATCCTGCAGCAAGCCCGCCGGGAGATGGAGGCCCAGCAGGCTGCCC
  TCGACCCTGCCTTAAAGCAGGCACCACTGTCCCAGAGTGACATCACCATCCTCACCCCCAAGCTTCTGTC
  CACCTCGCCCATGCCCACCGTGTCCAGCTACCCACCTCTCGCCATCTCCCTGAAGAAGCCCTCCGCAGCT
  CCTGAGGCCGGTGCCTCTGCTCTGCCGAACCCCCCGGCCCTCAAAAAGGAGGCCCAGGACGCCCCCGGGC
  TGGACCCCCAGGGAGCAGCCGATTGTGCACAAGGGGTCCTGAGACAGGTGAAAAATGAGGTGGGCCGCAG
  CGGTGCCTGGAAGGACCACTGGTGGAGCGCGGTGCAGCCGGAGAGAAGAAATGCCGCCTCCTCCGAGGAG
  GCCAAGGCCGAAGAAACGGGCGGCGGGAAAGAGAAGGGCAGCGGTGGCAGCGGAGGTGGCAGCCAGCCTC
  GGGCCGAGCGCAGTCAGCTCCAGGGACCCTCGTCGTCAGAGTACTGGAAGGAGTGGCCCAGCGCTGAGTC
  CCCATACTCCCAGAGCTCAGAGCTGAGTCTGACCGGGGCCAGCCGCAGCGAGACACCACAGAACAGCCCC
  CTGCCATCCTCCCCGATCGTGCCCATGTCCAAGCCCACCAAGCCCTCGGTCCCCCCGCTGACCCCCGAGC
  AGTACGAGGTCTACATGTACCAGGAGGTGGACACCATCGAGCTCACCCGGCAGGTTAAGGAAAAGCTGGC
  CAAGAACGGCATCTGCCAGAGAATCTTCGGGGAGAAGGTGCTGGGCCTGTCCCAGGGCAGCGTCAGCGAC
  ATGCTGTCCCGACCGAAGCCATGGAGCAAGCTGACGCAGAAAGGCCGAGAACCCTTCATCCGGATGCAGC
  TCTGGCTGAACGGCGAGCTAGGCCAGGGTGTTCTACCCGTCCAGGGCCAGCAGCAAGGGCCAGTCCTCCA
  CTCCGTGACATCGCTCCAGGACCCGCTGCAGCAGGGCTGTGTGAGCTCAGAAAGCACTCCAAAGACCTCC
  GCCAGCTGCAGCCCTGCCCCTGAGTCCCCGATGAGTTCCAGTGAGTCGGTGAAGAGCCTGACCGAGCTGG
  TCCAGCAGCCCTGTCCCCCCATCGAGGCGAGCAAGGACAGCAAGCCACCAGAGCCCAGTGACCCGCCAGC
  ATCCGACTCCCAGCCCACAACCCCGCTGCCTCTCTCCGGACACTCGGCCCTCAGCATCCAAGAATTAGTA
  GCCATGTCCCCGGAGCTGGACACCTACGGCATAACCAAGCGGGTGAAGGAGGTGCTGACGGACAACAACC
  TCGGCCAGCGCTTATTTGGGGAGACCATCTTAGGGCTCACCCAAGGCTCTGTCTCTGACCTCCTTGCCCG
  CCCCAAACCCTGGCATAAGCTCAGTCTGAAAGGACGAGAGCCCTTCGTCCGGATGCAGCTGTGGCTGAAC
  GACCCCAACAATGTGGAGAAGCTGATGGACATGAAACGGATGGAGAAGAAAGCCTACATGAAGCGGCGGC
  ACAGCTCAGTCAGTGACAGCCAGCCCTGCGAACCGCCCTCTGTCGGCACCGAGTACAGCCAGGGCGCCAG
  CCCCCAGCCCCAGCACCAGCTGAAGAAACCCCGGGTGGTGCTGGCTCCGGAGGAGAAGGAGGCGCTGAAA
  CGAGCGTATCAGCAAAAGCCATACCCGTCACCAAAAACCATCGAAGACCTCGCCACCCAGCTCAACCTGA
  AAACCAGCACCGTCATCAACTGGTTCCACAACTACAGGTCTCGGATCCGCAGAGAACTGTTCATTGAGGA
  AATTCAGGCCGGGAGTCAGGGCCAGGCGGGCGCCAGCGACTCACCCTCGGCCCGCAGCGGCCGGGCGGCG
  CCCAGCTCGGAGGGCGACAGCTGCGACGGCGTGGAGGCCACTGAGGGCCCAGGCAGCGCCGACACCGAGG
  AGCCCAAGTCTCAGGGAGAGGCCGAGCGGGAGGAGGTGCCGCGGCCGGCGGAGCAGACGGAGCCGCCGCC
  CTCGGGGACCCCGGGCCCGGACGACGCCCGCGACGACGACCACGAGGGAGGCCCCGTGGAAGGCCCGGGG
  CCCCTGCCCAGCCCCGCCTCCGCGACCGCCACCGCCGCGCCCGCGGCCCCCGAGGACGCCGCTACCTCAG
  CCGCCGCCGCGCCGGGGGAGGGCCCCGCGGCCCCGAGCTCCGCGCCGCCGCCCAGCAACAGCAGCAGCAG
  CAGCGCCCCCCGCAGGCCCAGCTCGCTGCAGAGCCTTTTCGGCCTCCCCGAGGCCGCGGGCGCCCGGGAC
  TCGCGCGACAACCCCCTGCGCAAGAAGAAGGCCGCGAACTTGAACAGCATCATCCACCGCCTGGAGAAGG
  CCGCCAGCCGGGAGGAACCTATCGAATGGGAGTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224512 representing NM_181552
  Red=Cloning site Green=Tags(s)
  
  MLCVAGARLKRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDLRKQVAPLLKSFQGEIDALSKR
  SKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIK
  ALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQSLQTALEKTRTE
  LFDLKTKYDEETTAKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVEQAI
  EVLTRSSLEVELAAKEREIAQLVEDVQRLQASLTKLRENSASQISQLEQQLSAKNSTLKQLEEKLKGQAD
  YEEVKKELNILKSMEFAPSEGAGTQDAAKPLEVLLLEKNRSLQSENAALRISNSDLSGSARRKGKDQPES
  RRPGSLPAPPPSQLPRNPGEQASNTNGTHQFSPAGLSQDFFSSSLASPSLPLASTGKFALNSLLQRQLMQ
  SFYSKAMQEAGSTSMIFSTGPYSTNSISSQSPLQQSPDVNGMAPSPSQSESAGSVSEGEEMDTAEIARQV
  KEQLIKHNIGQRIFGHYVLGLSQGSVSEILARPKPWNKLTVRGKEPFHKMKQFLSDEQNILALRSIQGRQ
  RENPGQSLNRLFQEVPKRRNGSEGNITTRIRASETGSDEAIKSILEQAKRELQVQKTAEPAQPSSASGSG
  NSDDAIRSILQQARREMEAQQAALDPALKQAPLSQSDITILTPKLLSTSPMPTVSSYPPLAISLKKPSAA
  PEAGASALPNPPALKKEAQDAPGLDPQGAADCAQGVLRQVKNEVGRSGAWKDHWWSAVQPERRNAASSEE
  AKAEETGGGKEKGSGGSGGGSQPRAERSQLQGPSSSEYWKEWPSAESPYSQSSELSLTGASRSETPQNSP
  LPSSPIVPMSKPTKPSVPPLTPEQYEVYMYQEVDTIELTRQVKEKLAKNGICQRIFGEKVLGLSQGSVSD
  MLSRPKPWSKLTQKGREPFIRMQLWLNGELGQGVLPVQGQQQGPVLHSVTSLQDPLQQGCVSSESTPKTS
  ASCSPAPESPMSSSESVKSLTELVQQPCPPIEASKDSKPPEPSDPPASDSQPTTPLPLSGHSALSIQELV
  AMSPELDTYGITKRVKEVLTDNNLGQRLFGETILGLTQGSVSDLLARPKPWHKLSLKGREPFVRMQLWLN
  DPNNVEKLMDMKRMEKKAYMKRRHSSVSDSQPCEPPSVGTEYSQGASPQPQHQLKKPRVVLAPEEKEALK
  RAYQQKPYPSPKTIEDLATQLNLKTSTVINWFHNYRSRIRRELFIEEIQAGSQGQAGASDSPSARSGRAA
  PSSEGDSCDGVEATEGPGSADTEEPKSQGEAEREEVPRPAEQTEPPPSGTPGPDDARDDDHEGGPVEGPG
  PLPSPASATATAAPAAPEDAATSAAAAPGEGPAAPSSAPPPSNSSSSSAPRRPSSLQSLFGLPEAAGARD
  SRDNPLRKKKAANLNSIIHRLEKAASREEPIEWEF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181552
• ORF Size: 4515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181552.1, NM_181552.2, NM_181552.3, NP_853530.1, NP_853530.2
• RefSeq Size: 13760 bp
• RefSeq ORF: 4518 bp
• Locus ID: 1523
• Cytogenetics: 7q22.1
• Protein Families: Transcription Factors, Transmembrane
• MW: 164.2 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Feb 2011]'