PXDN (NM_012293) Human Tagged ORF Clone

CAT#: RC224518

PXDN (Myc-DDK-tagged)-Human peroxidasin homolog (Drosophila) (PXDN)

Product Images

Western blot validation of overexpression lysate (Cat# LY415856) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224518 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PXDN protein (Cat# TP324518). The protein was produced from HEK293T cells transfected with PXDN cDNA clone (Cat# RC224518) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PXDN
• Synonyms: ASGD7; COPOA; D2S448; D2S448E; MG50; PRG2; PXN; VPO
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224518 representing NM_012293
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGCGCTCCAGGGGCCCCGGGCGCCGCTGCCTGTTGGCGCTCGTGCTGTTCTGCGCCTGGGGGA
  CGCTGGCCGTGGTGGCCCAGAAGCCGGGCGCAGGGTGTCCGAGCCGCTGCCTGTGCTTCCGCACCACCGT
  GCGCTGCATGCATCTGCTGCTGGAGGCCGTGCCCGCCGTGGCGCCGCAGACCTCCATCCTAGATCTTCGC
  TTTAACAGAATCAGAGAGATCCAACCTGGGGCATTCAGGCGGCTGAGGAACTTGAACACATTGCTTCTCA
  ATAATAATCAGATCAAGAGGATACCTAGTGGAGCATTTGAAGACTTGGAAAATTTAAAATATCTCTATCT
  GTACAAGAATGAGATCCAGTCAATTGACAGGCAAGCATTTAAGGGACTTGCCTCTCTAGAGCAACTATAC
  CTGCACTTTAATCAGATAGAAACTTTGGACCCAGATTCGTTCCAGCATCTCCCGAAGCTCGAGAGGCTAT
  TTTTGCATAACAACCGGATTACACATTTAGTTCCAGGGACATTTAATCACTTGGAATCTATGAAGAGATT
  GCGACTGGACTCAAACACACTTCACTGCGACTGTGAAATCCTGTGGTTGGCGGATTTGCTGAAAACCTAC
  GCGGAGTCGGGGAACGCGCAGGCAGCGGCCATCTGTGAATATCCCAGACGCATCCAGGGACGCTCAGTGG
  CAACCATCACCCCGGAAGAGCTGAACTGTGAAAGGCCCCGGATCACCTCCGAGCCCCAGGACGCAGATGT
  GACCTCGGGGAACACCGTGTACTTCACCTGCAGAGCCGAAGGCAACCCCAAGCCTGAGATCATCTGGCTG
  CGAAACAATAATGAGCTGAGCATGAAGACAGATTCCCGCCTAAACTTGCTGGACGATGGGACCCTGATGA
  TCCAGAACACACAGGAGACAGACCAGGGTATCTACCAGTGCATGGCAAAGAACGTGGCCGGAGAGGTGAA
  GACGCAAGAGGTGACCCTCAGGTACTTCGGGTCTCCAGCTCGACCCACTTTTGTAATCCAGCCACAGAAT
  ACAGAGGTGCTGGTTGGGGAGAGCGTCACGCTGGAGTGCAGCGCCACAGGCCACCCCCCGCCGCGGATCT
  CCTGGACGAGAGGTGACCGCACACCCTTGCCAGTTGACCCGCGGGTGAACATCACGCCTTCTGGCGGGCT
  TTACATACAGAACGTCGTACAGGGGGACAGCGGAGAGTATGCGTGCTCTGCGACCAACAACATTGACAGC
  GTCCATGCCACCGCTTTCATCATCGTCCAGGCTCTTCCTCAGTTCACTGTGACGCCTCAGGACAGAGTCG
  TTATTGAGGGCCAGACCGTGGATTTCCAGTGTGAAGCCAAGGGCAACCCGCCGCCCGTCATCGCCTGGAC
  CAAGGGAGGGAGCCAGCTCTCCGTGGACCGGCGGCACCTGGTCCTGTCATCGGGAACACTTAGAATCTCT
  GGTGTTGCCCTCCACGACCAGGGCCAGTACGAATGCCAGGCTGTCAACATCATCGGCTCCCAGAAGGTCG
  TGGCCCACCTGACTGTGCAGCCCAGAGTCACCCCAGTGTTTGCCAGCATTCCCAGCGACACAACAGTGGA
  GGTGGGCGCCAATGTGCAGCTCCCGTGCAGCTCCCAGGGCGAGCCCGAGCCAGCCATCACCTGGAACAAG
  GATGGGGTTCAGGTGACAGAAAGTGGAAAATTTCACATCAGCCCTGAAGGATTCTTGACCATCAATGACG
  TTGGCCCTGCAGACGCAGGTCGCTATGAGTGTGTGGCCCGGAACACCATTGGGTCGGCCTCGGTGAGCAT
  GGTGCTCAGTGTGAATGTTCCTGACGTCAGTCGAAATGGAGATCCGTTTGTAGCTACCTCCATCGTGGAA
  GCGATTGCGACTGTTGACAGAGCTATAAACTCAACCCGAACACATTTGTTTGACAGCCGTCCTCGTTCTC
  CAAATGATTTGCTGGCCTTGTTCCGGTATCCGAGGGATCCTTACACAGTTGAACAGGCACGGGCGGGAGA
  AATCTTTGAACGGACATTGCAGCTCATTCAGGAGCATGTACAGCATGGCTTGATGGTCGACCTCAACGGA
  ACAAGTTACCACTACAACGACCTGGTGTCTCCACAGTACCTGAACCTCATCGCAAACCTGTCGGGCTGTA
  CCGCCCACCGGCGCGTGAACAACTGCTCGGACATGTGCTTCCACCAGAAGTACCGGACGCACGACGGCAC
  CTGTAACAACCTGCAGCACCCCATGTGGGGCGCCTCGCTGACCGCCTTCGAGCGCCTGCTGAAATCCGTG
  TACGAGAATGGCTTCAACACCCCTCGGGGCATCAACCCCCACCGACTGTACAACGGGCACGCCCTTCCCA
  TGCCGCGCCTGGTGTCCACCACCCTGATCGGGACGGAGACCGTCACACCCGACGAGCAGTTCACCCACAT
  GCTGATGCAGTGGGGCCAGTTCCTGGACCACGACCTCGACTCCACGGTGGTGGCCCTGAGCCAGGCACGC
  TTCTCCGACGGACAGCACTGCAGCAACGTGTGCAGCAACGACCCCCCCTGCTTCTCTGTCATGATCCCCC
  CCAATGACTCCCGGGCCAGGAGCGGGGCCCGCTGCATGTTCTTCGTGCGCTCCAGCCCTGTGTGCGGCAG
  CGGCATGACTTCGCTGCTCATGAACTCCGTGTACCCGCGGGAGCAGATCAACCAGCTCACCTCCTACATA
  GACGCATCCAACGTGTACGGGAGCACGGAGCATGAGGCCCGCAGCATCCGCGACCTGGCCAGCCACCGCG
  GCCTGCTGCGGCAGGGCATCGTGCAGCGGTCCGGGAAGCCGCTGCTCCCCTTCGCCACCGGGCCGCCCAC
  GGAGTGCATGCGGGACGAGAACGAGAGCCCCATCCCCTGCTTCCTGGCCGGGGACCACCGCGCCAACGAG
  CAGCTGGGCCTGACCAGCATGCACACGCTGTGGTTCCGCGAGCACAACCGCATTGCCACGGAGCTGCTCA
  AGCTGAACCCGCACTGGGACGGCGACACCATCTACTATGAGACCAGGAAGATCGTGGGTGCGGAGATCCA
  GCACATCACCTACCAGCACTGGCTCCCGAAGATCCTGGGGGAGGTGGGCATGAGGACGCTGGGAGAGTAC
  CACGGCTACGACCCCGGCATCAATGCTGGCATCTTCAACGCCTTCGCCACCGCGGCCTTCAGGTTTGGCC
  ACACGCTTGTCAACCCACTGCTTTACCGGCTGGACGAGAACTTCCAGCCCATTGCACAAGATCACCTCCC
  CCTTCACAAAGCTTTCTTCTCTCCCTTCCGGATTGTGAATGAGGGCGGCATCGATCCGCTTCTCAGGGGG
  CTGTTCGGGGTGGCGGGGAAAATGCGTGTGCCCTCGCAGCTGCTGAACACGGAGCTCACGGAGCGGCTGT
  TCTCCATGGCACACACGGTGGCTCTGGACCTGGCGGCCATCAACATCCAGCGGGGCCGGGACCACGGGAT
  CCCACCCTACCACGACTACAGGGTCTACTGCAATCTATCGGCGGCACACACGTTCGAGGACCTGAAAAAT
  GAGATTAAAAACCCTGAGATCCGGGAGAAACTGAAAAGGTTGTATGGCTCGACACTCAACATCGACCTGT
  TTCCGGCGCTCGTGGTGGAGGACCTGGTGCCTGGCAGCCGGCTGGGCCCCACCCTGATGTGTCTTCTCAG
  CACACAGTTCAAGCGCCTGCGAGATGGGGACAGGTTGTGGTATGAGAACCCTGGGGTGTTCTCCCCGGCC
  CAGCTGACTCAGATCAAGCAGACGTCGCTGGCCAGGATCCTATGCGACAACGCGGACAACATCACCCGGG
  TGCAGAGCGACGTGTTCAGGGTGGCGGAGTTCCCTCACGGCTACGGCAGCTGTGACGAGATCCCCAGGGT
  AGACCTCCGGGTGTGGCAGGACTGCTGTGAAGACTGTAGGACCAGGGGGCAGTTCAATGCCTTTTCCTAT
  CATTTCCGAGGCAGACGGTCTCTTGAGTTCAGCTACCAGGAGGACAAGCCGACCAAGAAAACAAGACCAC
  GGAAAATACCCAGTGTTGGGAGACAGGGGGAACATCTCAGCAACAGCACCTCAGCCTTCAGCACACGCTC
  AGATGCATCTGGGACAAATGACTTCAGAGAGTTTGTTCTGGAAATGCAGAAGACCATCACAGACCTCAGA
  ACACAGATAAAGAAACTTGAATCACGGCTCAGTACCACAGAGTGCGTGGATGCCGGGGGCGAATCTCACG
  CCAACAACACCAAGTGGAAAAAAGATGCATGCACCATTTGTGAATGCAAAGACGGGCAGGTCACCTGCTT
  CGTGGAAGCTTGCCCCCCTGCCACCTGTGCTGTCCCCGTGAACATCCCAGGGGCCTGCTGTCCAGTCTGC
  TTACAGAAGAGGGCGGAGGAAAAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224518 representing NM_012293
  Red=Cloning site Green=Tags(s)
  
  MAKRSRGPGRRCLLALVLFCAWGTLAVVAQKPGAGCPSRCLCFRTTVRCMHLLLEAVPAVAPQTSILDLR
  FNRIREIQPGAFRRLRNLNTLLLNNNQIKRIPSGAFEDLENLKYLYLYKNEIQSIDRQAFKGLASLEQLY
  LHFNQIETLDPDSFQHLPKLERLFLHNNRITHLVPGTFNHLESMKRLRLDSNTLHCDCEILWLADLLKTY
  AESGNAQAAAICEYPRRIQGRSVATITPEELNCERPRITSEPQDADVTSGNTVYFTCRAEGNPKPEIIWL
  RNNNELSMKTDSRLNLLDDGTLMIQNTQETDQGIYQCMAKNVAGEVKTQEVTLRYFGSPARPTFVIQPQN
  TEVLVGESVTLECSATGHPPPRISWTRGDRTPLPVDPRVNITPSGGLYIQNVVQGDSGEYACSATNNIDS
  VHATAFIIVQALPQFTVTPQDRVVIEGQTVDFQCEAKGNPPPVIAWTKGGSQLSVDRRHLVLSSGTLRIS
  GVALHDQGQYECQAVNIIGSQKVVAHLTVQPRVTPVFASIPSDTTVEVGANVQLPCSSQGEPEPAITWNK
  DGVQVTESGKFHISPEGFLTINDVGPADAGRYECVARNTIGSASVSMVLSVNVPDVSRNGDPFVATSIVE
  AIATVDRAINSTRTHLFDSRPRSPNDLLALFRYPRDPYTVEQARAGEIFERTLQLIQEHVQHGLMVDLNG
  TSYHYNDLVSPQYLNLIANLSGCTAHRRVNNCSDMCFHQKYRTHDGTCNNLQHPMWGASLTAFERLLKSV
  YENGFNTPRGINPHRLYNGHALPMPRLVSTTLIGTETVTPDEQFTHMLMQWGQFLDHDLDSTVVALSQAR
  FSDGQHCSNVCSNDPPCFSVMIPPNDSRARSGARCMFFVRSSPVCGSGMTSLLMNSVYPREQINQLTSYI
  DASNVYGSTEHEARSIRDLASHRGLLRQGIVQRSGKPLLPFATGPPTECMRDENESPIPCFLAGDHRANE
  QLGLTSMHTLWFREHNRIATELLKLNPHWDGDTIYYETRKIVGAEIQHITYQHWLPKILGEVGMRTLGEY
  HGYDPGINAGIFNAFATAAFRFGHTLVNPLLYRLDENFQPIAQDHLPLHKAFFSPFRIVNEGGIDPLLRG
  LFGVAGKMRVPSQLLNTELTERLFSMAHTVALDLAAINIQRGRDHGIPPYHDYRVYCNLSAAHTFEDLKN
  EIKNPEIREKLKRLYGSTLNIDLFPALVVEDLVPGSRLGPTLMCLLSTQFKRLRDGDRLWYENPGVFSPA
  QLTQIKQTSLARILCDNADNITRVQSDVFRVAEFPHGYGSCDEIPRVDLRVWQDCCEDCRTRGQFNAFSY
  HFRGRRSLEFSYQEDKPTKKTRPRKIPSVGRQGEHLSNSTSAFSTRSDASGTNDFREFVLEMQKTITDLR
  TQIKKLESRLSTTECVDAGGESHANNTKWKKDACTICECKDGQVTCFVEACPPATCAVPVNIPGACCPVC
  LQKRAEEKP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012293
• ORF Size: 4437 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012293.1, NM_012293.2, NP_036425.1
• RefSeq Size: 6821
• RefSeq ORF: 4440
• Locus ID: 7837
• Protein Families: Druggable Genome, Transmembrane
• MW: 165.1 kDa
• Gene Summary: This gene encodes a heme-containing peroxidase that is secreted into the extracellular matrix. It is involved in extracellular matrix formation, and may function in the physiological and pathological fibrogenic response in fibrotic kidney. Mutations in this gene cause corneal opacification and other ocular anomalies, and also microphthalmia and anterior segment dysgenesis. [provided by RefSeq, Aug 2014]