LPP (NM_005578) Human Tagged ORF Clone

CAT#: RC224539

LPP (Myc-DDK-tagged)-Human LIM domain containing preferred translocation partner in lipoma (LPP), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417214) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224539 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified LPP protein (Cat# TP324539). The protein was produced from HEK293T cells transfected with LPP cDNA clone (Cat# RC224539) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LPP
• Synonyms: DKFZp779O0231; FLJ30652; FLJ41512
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224539 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCACCCATCTTGGCTGCCACCCAAAAGCACTGGTGAGCCCCTCGGCCATGTGCCTGCACGGATGG
  AGACCACCCATTCCTTTGGGAACCCCAGCATTTCAGTGTCTACACAACAGCCACCCAAAAAGTTTGCCCC
  GGTAGTTGCTCCAAAACCTAAGTACAACCCATACAAACAACCTGGAGGTGAGGGTGATTTTCTTCCACCC
  CCACCTCCACCTCTAGATGATTCCAGTGCCCTTCCATCTATCTCTGGAAACTTTCCTCCTCCACCACCTC
  TTGATGAAGAGGCTTTCAAAGTACAGGGGAATCCCGGAGGCAAGACACTTGAGGAGAGGCGCTCCAGCCT
  GGACGCTGAGATTGACTCCTTGACCAGCATCTTGGCTGACCTTGAGTGCAGCTCCCCCTATAAGCCTCGG
  CCTCCACAGAGCTCCACTGGTTCAACAGCCTCTCCTCCAGTTTCGACCCCAGTCACAGGACACAAGAGAA
  TGGTCATCCCGAACCAACCCCCTCTAACAGCAACCAAGAAGTCTACATTGAAACCACAGCCTGCACCCCA
  GGCTGGACCCATCCCTGTGGCTCCAATCGGAACACTCAAACCCCAGCCTCAGCCAGTCCCAGCCTCCTAC
  ACCACGGCCTCCACTTCTTCAAGGCCTACCTTTAATGTGCAGGTGAAGTCAGCCCAGCCCAGCCCTCATT
  ATATGGCTGCCCCTTCATCAGGACAAATTTATGGCTCAGGGCCCCAGGGCTATAACACTCAGCCAGTTCC
  TGTCTCTGGGCAGTGTCCACCTCCTTCAACACGGGGAGGCATGGATTATGCCTACATTCCACCACCAGGA
  CTTCAGCCGGAGCCTGGGTATGGGTATGCCCCCAACCAGGGACGCTATTATGAAGGCTACTATGCAGCAG
  GGCCAGGCTATGGGGGCAGAAATGACTCTGATCCTACCTATGGTCAACAAGGTCACCCAAATACCTGGAA
  ACGGGAACCAGGGTACACTCCTCCTGGAGCAGGGAACCAGAACCCTCCTGGGATGTATCCAGTCACTGGT
  CCCAAGAAGACCTATATCACAGATCCTGTTTCAGCCCCCTGTGCGCCACCATTGCAGCCAAAGGGTGGCC
  ATTCAGGGCAACTGGGGCCTTCGTCAGTTGCCCCTTCATTCCGCCCAGAGGATGAGCTTGAGCACCTGAC
  CAAAAAGATGCTGTATGACATGGAAAATCCACCTGCTGACGAATACTTTGGCCGCTGTGCTCGCTGTGGA
  GAAAACGTAGTTGGGGAAGGTACAGGATGCACTGCCATGGATCAGGTCTTCCACGTGGATTGTTTTACCT
  GCATCATCTGCAACAACAAGCTCCGAGGGCAGCCATTCTATGCTGTGGAAAAGAAAGCATACTGCGAGCC
  CTGCTACATTAATACTCTGGAGCAGTGCAATGTGTGTTCCAAGCCCATCATGGAGCGGATTCTCCGAGCC
  ACCGGGAAGGCCTATCATCCTCACTGTTTCACCTGCGTGATGTGCCACCGCAGCCTGGATGGGATCCCAT
  TCACTGTGGATGCTGGCGGGCTCATTCACTGCATTGAGGACTTCCACAAGAAATTTGCCCCGCGGTGTTC
  TGTGTGCAAGGAGCCTATTATGCCAGCCCCGGGCCAGGAGGAGACTGTCCGTATTGTGGCTTTGGATCGA
  GATTTCCATGTTCACTGCTACCGATGCGAGGATTGCGGTGGTCTCCTGTCTGAAGGAGATAACCAAGGCT
  GCTACCCCTTGGATGGGCACATCCTCTGCAAGACCTGCAACTCTGCCCGCATCAGGGTGTTGACCGCCAA
  GGCGAGCACTGACCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224539 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSHPSWLPPKSTGEPLGHVPARMETTHSFGNPSISVSTQQPPKKFAPVVAPKPKYNPYKQPGGEGDFLPP
  PPPPLDDSSALPSISGNFPPPPPLDEEAFKVQGNPGGKTLEERRSSLDAEIDSLTSILADLECSSPYKPR
  PPQSSTGSTASPPVSTPVTGHKRMVIPNQPPLTATKKSTLKPQPAPQAGPIPVAPIGTLKPQPQPVPASY
  TTASTSSRPTFNVQVKSAQPSPHYMAAPSSGQIYGSGPQGYNTQPVPVSGQCPPPSTRGGMDYAYIPPPG
  LQPEPGYGYAPNQGRYYEGYYAAGPGYGGRNDSDPTYGQQGHPNTWKREPGYTPPGAGNQNPPGMYPVTG
  PKKTYITDPVSAPCAPPLQPKGGHSGQLGPSSVAPSFRPEDELEHLTKKMLYDMENPPADEYFGRCARCG
  ENVVGEGTGCTAMDQVFHVDCFTCIICNNKLRGQPFYAVEKKAYCEPCYINTLEQCNVCSKPIMERILRA
  TGKAYHPHCFTCVMCHRSLDGIPFTVDAGGLIHCIEDFHKKFAPRCSVCKEPIMPAPGQEETVRIVALDR
  DFHVHCYRCEDCGGLLSEGDNQGCYPLDGHILCKTCNSARIRVLTAKASTDL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005578
• ORF Size: 1836 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005578.1, NM_005578.2, NM_005578.3, NM_005578.4, NP_005569.1
• RefSeq Size: 18296 bp
• RefSeq ORF: 1839 bp
• Locus ID: 4026
• Cytogenetics: 3q27.3-q28
• Domains: LIM
• MW: 65.7 kDa
• Gene Summary: 'This gene encodes a member of a subfamily of LIM domain proteins that are characterized by an N-terminal proline-rich region and three C-terminal LIM domains. The encoded protein localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility. This protein also shuttles through the nucleus and may function as a transcriptional co-activator. This gene is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]'