CELF2 (NM_006561) Human Tagged ORF Clone

CAT#: RC224548

CELF2 (Myc-DDK-tagged)-Human CUGBP, Elav-like family member 2 (CELF2), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY416560) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224548 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CELF2 protein (Cat# TP324548). The protein was produced from HEK293T cells transfected with CELF2 cDNA clone (Cat# RC224548) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CELF2
• Synonyms: BRUNOL3; CELF-2; CUG-BP2; CUGBP2; ETR-3; ETR3; NAPOR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224548 representing NM_006561
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTTCTGCCTTCAAGCTGGATTTCCTCCCGGACATGATGGTCGAGGGCCGCCTGCTCGTTCCTGACA
  GAATTAACGGCACAGCCAACAAGATGAACGGAGCTTTGGATCACTCAGACCAACCAGACCCAGATGCCAT
  TAAGATGTTTGTCGGACAGATCCCCCGGTCATGGTCGGAAAAGGAGCTGAAAGAACTTTTTGAGCCTTAC
  GGAGCCGTCTACCAGATCAACGTCCTCCGGGACCGGAGTCAGAACCCTCCGCAGAGTAAAGGTTGTTGTT
  TCGTAACATTTTATACAAGAAAAGCTGCACTTGAGGCCCAGAATGCACTGCACAATATTAAAACTTTACC
  TGGGATGCATCATCCCATTCAGATGAAACCTGCAGATAGTGAAAAGTCCAACGCTGTGGAAGACAGAAAA
  TTGTTCATAGGAATGGTATCGAAGAAATGTAATGAGAACGACATCAGGGTGATGTTCTCTCCATTTGGCC
  AGATAGAAGAATGCCGGATCCTCCGGGGACCTGATGGGCTGAGTCGAGGCTGTGCGTTTGTCACATTTTC
  TACAAGGGCAATGGCACAGAATGCAATCAAAGCCATGCATCAGTCTCAGACCATGGAGGGCTGCTCTTCA
  CCTATCGTGGTGAAGTTTGCTGACACTCAGAAGGACAAAGAGCAAAGGCGCCTCCAGCAGCAGCTCGCTC
  AGCAGATGCAGCAGCTCAACACTGCCACCTGGGGGAACCTGACAGGGCTGGGCGGACTGACCCCACAGTA
  TCTGGCGCTCCTGCAGCAGGCCACCTCCTCCAGCAACCTGGGTGCGTTCAGCGGCATTCAACAAATGGCA
  GGCATGAATGCTTTACAGTTGCAGAACCTGGCGACGCTGGCTGCTGCTGCAGCTGCGGCCCAGACCTCAG
  CCACCAGCACCAATGCAAACCCTCTCTCTACCACGAGCAGCGCCCTGGGAGCCCTCACGAGTCCCGTGGC
  TGCTTCAACCCCCAACTCCACTGCTGGTGCAGCCATGAACTCCTTGACCTCTCTCGGGACTCTGCAAGGA
  CTGGCTGGAGCCACTGTTGGACTGAATAATATTAATGCACTAGCAGTTGCTCAAATGCTCTCAGGTATGG
  CGGCTCTGAATGGAGGACTTGGCGCCACAGGCTTGACGAATGGCACGGCTGGCACCATGGACGCCCTCAC
  CCAGGCCTACTCAGGAATTCAACAGTACGCAGCCGCCGCGCTGCCCACTCTGTACAGCCAGAGCCTGCTG
  CAGCAGCAGAGCGCTGCAGGCAGCCAGAAGGAAGGTCCAGAGGGGGCAAACCTCTTTATTTACCACCTTC
  CACAGGAATTTGGAGACCAGGACATTCTGCAGATGTTCATGCCTTTTGGAAATGTTATCTCTGCTAAAGT
  CTTCATTGACAAACAGACCAATCTGAGCAAGTGCTTTGGTTTTGTTAGCTACGACAATCCAGTCTCTGCA
  CAAGCTGCTATCCAAGCTATGAATGGCTTTCAGATCGGCATGAAACGCTTGAAGGTGCAGCTGAAGCGTT
  CCAAAAACGACAGCAAACCTTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224548 representing NM_006561
  Red=Cloning site Green=Tags(s)
  
  MTSAFKLDFLPDMMVEGRLLVPDRINGTANKMNGALDHSDQPDPDAIKMFVGQIPRSWSEKELKELFEPY
  GAVYQINVLRDRSQNPPQSKGCCFVTFYTRKAALEAQNALHNIKTLPGMHHPIQMKPADSEKSNAVEDRK
  LFIGMVSKKCNENDIRVMFSPFGQIEECRILRGPDGLSRGCAFVTFSTRAMAQNAIKAMHQSQTMEGCSS
  PIVVKFADTQKDKEQRRLQQQLAQQMQQLNTATWGNLTGLGGLTPQYLALLQQATSSSNLGAFSGIQQMA
  GMNALQLQNLATLAAAAAAAQTSATSTNANPLSTTSSALGALTSPVAASTPNSTAGAAMNSLTSLGTLQG
  LAGATVGLNNINALAVAQMLSGMAALNGGLGATGLTNGTAGTMDALTQAYSGIQQYAAAALPTLYSQSLL
  QQQSAAGSQKEGPEGANLFIYHLPQEFGDQDILQMFMPFGNVISAKVFIDKQTNLSKCFGFVSYDNPVSA
  QAAIQAMNGFQIGMKRLKVQLKRSKNDSKPY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006561
• ORF Size: 1563 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006561.3, NP_006552.3
• RefSeq Size: 8005
• RefSeq ORF: 1566
• Locus ID: 10659
• Domains: RRM
• MW: 55.6 kDa
• Gene Summary: Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]