ALKBH6 (NM_032878) Human Tagged ORF Clone

CAT#: RC224553

ALKBH6 (Myc-DDK-tagged)-Human alkB, alkylation repair homolog 6 (E. coli) (ALKBH6), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY409909) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224553 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ALKBH6 protein (Cat# TP324553). The protein was produced from HEK293T cells transfected with ALKBH6 cDNA clone (Cat# RC224553) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALKBH6
• Synonyms: ABH6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224553 representing NM_032878
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGGAGGGGGATGGGGATGTTGAATTTGGAAATTGGAGGGGACGCTGGTGGACGGATTGGGTGCA
  AGGAGTTGGTGTTGATGGAGGAGCAGGACGCCAGAGTCCCAGCCCTGGAACCGTTCAGAGTGGAGCAGGC
  ACCACCTGTAATCTACTATGTCCCTGACTTCATCTCCAAAGAAGAGGAGGAGTATTTGCTTCGACAGGTT
  TTTAATGCCCCAAAGCCAAAGTGGACCCAGCTCTCTGGGAGAAAGTTACAGAACTGGGGTGGGCTTCCTC
  ATCCCCGAGGGATGGTTCCTGAGCGGCTGCCCCCATGGCTCCAGCGCTACGTGGACAAAGTGTCAAACCT
  CAGCCTCTTTGGAGGCCTCCCAGCTAACCATGTCCTCGTGAACCAGTATCTGCCTGGGGAGGGCATCATG
  CCCCACGAGGACGGACCACTGTACTACCCGACTGTCAGCACCATCAGCCTGGGCTCCCACACCGTGCTGG
  ACTTCTACGAGCCGCGGCGGCCAGAGGACGATGACCCTACAGAACAGCCTCGGCCTCCGCCCCGGCCCAC
  CACCTCGCTACTGCTGGAACCGCGCAGCCTGCTGGTGCTCCGCGGCCCCGCCTACACGCGTCTTCTCCAC
  GGCATCGCCGCCGCCCGCGTAGACGCGCTGGACGCCGCCTCCTCGCCGCCCAATGCGGCAGCCTGCCCGT
  CGGCGCGGCCGGGAGCCTGCCTGGTGCGCGGCACCCGGGTCTCGCTGACCATCCGCCGCGTGCCCCGCGT
  GCTGCGCGCCGGCCTCCTGCTGGGCAAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224553 representing NM_032878
  Red=Cloning site Green=Tags(s)
  
  MAGRGMGMLNLEIGGDAGGRIGCKELVLMEEQDARVPALEPFRVEQAPPVIYYVPDFISKEEEEYLLRQV
  FNAPKPKWTQLSGRKLQNWGGLPHPRGMVPERLPPWLQRYVDKVSNLSLFGGLPANHVLVNQYLPGEGIM
  PHEDGPLYYPTVSTISLGSHTVLDFYEPRRPEDDDPTEQPRPPPRPTTSLLLEPRSLLVLRGPAYTRLLH
  GIAAARVDALDAASSPPNAAACPSARPGACLVRGTRVSLTIRRVPRVLRAGLLLGK
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032878
• ORF Size: 798 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032878.3, NP_116267.3
• RefSeq Size: 980
• RefSeq ORF: 801
• Locus ID: 84964
• Domains: 2OG-FeII_Oxy
• MW: 29.1 kDa