EIF2B4 (NM_172195) Human Tagged ORF Clone

CAT#: RC224602

EIF2B4 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2B, subunit 4 delta, 67kDa (EIF2B4), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY406769) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224602 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EIF2B4
• Synonyms: EIF-2B; EIF2B; EIF2Bdelta
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224602 representing NM_172195
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAACCCAGCAGCCGGCTGCGCCGAGTACTCGTGCCCCCAAACCCTCCCGGAGTCTCTCTGGCTCAC
  TTTGTGCCCTGTTTTCTGATGCAGACTCGGGATCCGGGATGAAGGCGGAGCTTCCCCCTGGGCCTGGGGC
  AGTGGGGAGGGAAATGACCAAAGAAGAAAAGCTGCAGCTTCGGAAGGAAAAGAAACAGCAGAAGAAGAAA
  CGGAAGGAAGAAAAGGGGGCAGAACCAGAGACTGGCTCTGCTGTATCTGCAGCCCAATGTCAAGGCCCAA
  CCAGAGAACTGCCAGAATCGGGCATTCAGTTGGGCACTCCTCGGGAGAAAGTTCCAGCTGGTCGGAGTAA
  GGCCGAACTTCGGGCTGAGCGTCGAGCCAAGCAGGAGGCCGAGCGGGCCCTGAAACAGGCAAGAAAAGGG
  GAACAAGGAGGACCACCTCCTAAGGCCAGCCCCAGCACAGCTGGAGAAACCCCCTCAGGAGTGAAGCGTC
  TCCCTGAGTACCCTCAGGTTGATGACCTACTTCTGAGAAGGCTTGTTAAAAAACCAGAGCGTCAACAGGT
  TCCTACACGAAAGGATTATGGATCCAAAGTCAGTCTCTTCTCTCACCTACCCCAGTACAGCAGACAAAAC
  TCTCTGACCCAGTTTATGAGCATCCCATCCTCTGTGATCCACCCAGCCATGGTGCGACTCGGCCTGCAGT
  ACTCCCAGGGCCTGGTCAGTGGCTCCAATGCCCGGTGTATTGCCCTGCTTCGTGCCTTGCAGCAGGTGAT
  TCAGGATTACACAACACCGCCTAATGAAGAACTCTCCAGGGATCTAGTGAATAAACTAAAACCCTACATG
  AGCTTCCTGACTCAGTGCCGTCCCCTGTCAGCGAGCATGCACAACGCCATCAAGTTCCTTAACAAGGAAA
  TCACCAGTGTGGGCAGTTCCAAGCGGGAAGAGGAGGCCAAGTCAGAACTTCGAGCAGCCATTGATCGGTA
  TGTGCAAGAGAAGATTGTGCTAGCAGCTCAGGCAATTTCACGCTTTGCTTACCAGAAGATCAGTAATGGA
  GATGTGATCCTGGTATATGGATGCTCATCTCTGGTATCACGAATTCTTCAGGAGGCTTGGACAGAGGGCC
  GGCGGTTTCGGGTGGTAGTGGTGGACAGCCGGCCATGGCTGGAAGGAAGGCACACACTACGTTCTCTAGT
  CCATGCTGGTGTCCCAGCCTCCTACCTGCTGATTCCTGCAGCCTCCTATGTGCTCCCAGAGGTTTCCAAG
  GTGCTATTGGGAGCTCATGCACTCTTGGCCAACGGGTCTGTGATGTCACGGGTAGGGACAGCACAGTTAG
  CCCTGGTGGCTCGAGCCCATAATGTACCAGTGCTGGTTTGCTGTGAAACATACAAGTTCTGTGAGCGTGT
  GCAGACTGATGCCTTTGTCTCTAATGAGCTAGATGACCCTGATGATCTGCAATGTAAGCGGGGAGAACAT
  GTTGCGCTGGCTAACTGGCAGAACCACGCATCCCTACGGTTGTTGAATCTAGTCTATGATGTGACTCCCC
  CAGAGCTTGTGGATCTGGTGATCACGGAGCTGGGGATGATCCCTTGCAGTTCTGTACCTGTTGTTCTACG
  AGTCAAGAGCAGTGACCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224602 representing NM_172195
  Red=Cloning site Green=Tags(s)
  
  MPTQQPAAPSTRAPKPSRSLSGSLCALFSDADSGSGMKAELPPGPGAVGREMTKEEKLQLRKEKKQQKKK
  RKEEKGAEPETGSAVSAAQCQGPTRELPESGIQLGTPREKVPAGRSKAELRAERRAKQEAERALKQARKG
  EQGGPPPKASPSTAGETPSGVKRLPEYPQVDDLLLRRLVKKPERQQVPTRKDYGSKVSLFSHLPQYSRQN
  SLTQFMSIPSSVIHPAMVRLGLQYSQGLVSGSNARCIALLRALQQVIQDYTTPPNEELSRDLVNKLKPYM
  SFLTQCRPLSASMHNAIKFLNKEITSVGSSKREEEAKSELRAAIDRYVQEKIVLAAQAISRFAYQKISNG
  DVILVYGCSSLVSRILQEAWTEGRRFRVVVVDSRPWLEGRHTLRSLVHAGVPASYLLIPAASYVLPEVSK
  VLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGEH
  VALANWQNHASLRLLNLVYDVTPPELVDLVITELGMIPCSSVPVVLRVKSSDQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172195
• ORF Size: 1629 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_172195.1, NM_172195.2, NM_172195.3, NP_751945.1, NP_751945.2
• RefSeq Size: 1726
• RefSeq ORF: 1632
• Locus ID: 8890
• MW: 59.4 kDa
• Gene Summary: Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]