PTCH2 (NM_003738) Human Tagged ORF Clone

CAT#: RC224642

PTCH2 (Myc-DDK-tagged)-Human patched 2 (PTCH2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY418463) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224642 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PTCH2
• Synonyms: PTC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224642 representing NM_003738
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCGATCGCCGCCCCTCAGAGAGCTGCCCCCGAGTTACACACCCCCAGCTCGAACCGCAGCACCCC
  AGATCCTAGCTGGGAGCCTGAAGGCTCCACTCTGGCTTCGTGCTTACTTCCAGGGCCTGCTCTTCTCTCT
  GGGATGCGGGATCCAGAGACATTGTGGCAAAGTGCTCTTTCTGGGACTGTTGGCCTTTGGGGCCCTGGCA
  TTAGGTCTCCGCATGGCCATTATTGAGACAAACTTGGAACAGCTCTGGGTAGAAGTGGGCAGCCGGGTGA
  GCCAGGAGCTGCATTACACCAAGGAGAAGCTGGGGGAGGAGGCTGCATACACCTCTCAGATGCTGATACA
  GACCGCACGCCAGGAGGGAGAGAACATCCTCACACCCGAAGCACTTGGCCTCCACCTCCAGGCAGCCCTC
  ACTGCCAGTAAAGTCCAAGTATCACTCTATGGGAAGTCCTGGGATTTGAACAAAATCTGCTACAAGTCAG
  GAGTTCCCCTTATTGAAAATGGAATGATTGAGCGGATGATTGAGAAGCTGTTTCCGTGCGTGATCCTCAC
  CCCCCTCGACTGCTTCTGGGAGGGAGCCAAACTCCAAGGGGGCTCCGCCTACCTGCCCGGCCGCCCGGAT
  ATCCAGTGGACCAACCTGGATCCAGAGCAGCTGCTGGAGGAGCTGGGTCCCTTTGCCTCCCTTGAGGGCT
  TCCGGGAGCTGCTAGACAAGGCACAGGTGGGCCAGGCCTACGTGGGGCGGCCCTGTCTGCACCCTGATGA
  CCTCCACTGCCCACCTAGTGCCCCCAACCATCACAGCAGGCAGGCTCCCAATGTGGCTCACGAGCTGAGT
  GGGGGCTGCCATGGCTTCTCCCACAAATTCATGCACTGGCAGGAGGAATTGCTGCTGGGAGGCATGGCCA
  GAGACCCCCAAGGAGAGCTGCTGAGGGCAGAGGCCCTGCAGAGCACCTTCTTGCTGATGAGTCCCCGCCA
  GCTGTACGAGCATTTCCGGGGTGACTATCAGACACATGACATTGGCTGGAGTGAGGAGCAGGCCAGCACA
  GTGCTACAAGCCTGGCAGCGGCGCTTTGTGCAGCTGGCCCAGGAGGCCCTGCCTGAGAACGCTTCCCAGC
  AGATCCATGCCTTCTCCTCCACCACCCTGGATGACATCCTGCATGCGTTCTCTGAAGTCAGTGCTGCCCG
  TGTGGTGGGAGGCTATCTGCTCATGCTGGCCTATGCCTGTGTGACCATGCTGCGGTGGGACTGCGCCCAG
  TCCCAGGGTTCCGTGGGCCTTGCCGGGGTACTGCTGGTGGCCCTGGCGGTGGCCTCAGGCCTTGGGCTCT
  GTGCCCTGCTCGGCATCACCTTCAATGCTGCCACTACCCAGGTGCTGCCCTTCTTGGCTCTGGGAATCGG
  CGTGGATGACGTATTCCTGCTGGCGCATGCCTTCACAGAGGCTCTGCCTGGCACCCCTCTCCAGGAGCGC
  ATGGGCGAGTGTCTGCAGCGCACGGGCACCAGTGTCGTACTCACATCCATCAACAACATGGCCGCCTTCC
  TCATGGCTGCCCTCGTTCCCATCCCTGCGCTGCGAGCCTTCTCCCTACAGGCGGCCATAGTGGTTGGCTG
  CACCTTTGTAGCCGTGATGCTTGTCTTCCCAGCCATCCTCAGCCTGGACCTACGGCGGCGCCACTGCCAG
  CGCCTTGATGTGCTCTGCTGCTTCTCCAGTCCCTGCTCTGCTCAGGTGATTCAGATCCTGCCCCAGGAGC
  TGGGGGACGGGACAGTACCAGTGGGCATTGCCCACCTCACTGCCACAGTTCAAGCCTTTACCCACTGTGA
  AGCCAGCAGCCAGCATGTGGTCACCATCCTGCCTCCCCAAGCCCACCTGGTGCCCCCACCTTCTGACCCA
  CTGGGCTCTGAGCTCTTCAGCCCTGGAGGGTCCACACGGGACCTTCTAGGCCAGGAGGAGGAGACAAGGC
  AGAAGGCAGCCTGCAAGTCCCTGCCCTGTGCCCGCTGGAATCTTGCCCATTTCGCCCGCTATCAGTTTGC
  CCCGTTGCTGCTCCAGTCACATGCTAAGGCCATCGTGCTGGTGCTCTTTGGTGCTCTTCTGGGCCTGAGC
  CTCTACGGAGCCACCTTGGTGCAAGACGGCCTGGCCCTGACGGATGTGGTGCCTCGGGGCACCAAGGAGC
  ATGCCTTCCTGAGCGCCCAGCTCAGGTACTTCTCCCTGTACGAGGTGGCCCTGGTGACCCAGGGTGGCTT
  TGACTACGCCCACTCCCAACGCGCCCTCTTTGATCTGCACCAGCGCTTCAGTTCCCTCAAGGCGGTGCTG
  CCCCCACCGGCCACCCAGGCACCCCGCACCTGGCTGCACTATTACCGCAACTGGCTACAGGGAATCCAGG
  CTGCCTTTGACCAGGACTGGGCTTCTGGGCGCATCACCCGCCACTCGTACCGCAATGGCTCTGAGGATGG
  GGCCCTGGCCTACAAGCTGCTCATCCAGACTGGAGACGCCCAGGAGCCTCTGGATTTCAGCCAGCTGACC
  ACAAGGAAGCTGGTGGACAGAGAGGGACTGATTCCACCCGAGCTCTTCTACATGGGGCTGACCGTGTGGG
  TGAGCAGTGACCCCCTGGGTCTGGCAGCCTCACAGGCCAACTTCTACCCCCCACCTCCTGAATGGCTGCA
  CGACAAATACGACACCACGGGGGAGAACCTTCGCATCCCGCCAGCTCAGCCCTTGGAGTTTGCCCAGTTC
  CCCTTCCTGCTGCGTGGCCTCCAGAAGACTGCAGACTTTGTGGAGGCCATCGAGGGGGCCCGGGCAGCAT
  GCGCAGAGGCCGGCCAGGCTGGGGTGCACGCCTACCCCAGCGGCTCCCCCTTCCTCTTCTGGGAACAGTA
  TCTGGGCCTGCGGCGCTGCTTCCTGCTGGCCGTCTGCATCCTGCTGGTGTGCACTTTCCTCGTCTGTGCT
  CTGCTGCTCCTCAACCCCTGGACGGCTGGCCTCATAGTGCTGGTCCTGGCGATGATGACAGTGGAACTCT
  TTGGTATCATGGGTTTCCTGGGCATCAAGCTGAGTGCCATCCCCGTGGTGATCCTTGTGGCCTCTGTAGG
  CATTGGCGTTGAGTTCACAGTCCACGTGGCTCTGGGCTTCCTGACCACCCAGGGCAGCCGGAACCTGCGG
  GCCGCCCATGCCCTTGAGCACACATTTGCCCCCGTGACCGATGGGGCCATCTCCACATTGCTGGGTCTGC
  TCATGCTTGCTGGTTCCCACTTTGACTTCATTGTAAGGTACTTCTTTGCGGCGCTGACAGTGCTCACGCT
  CCTGGGCCTCCTCCATGGACTCGTGCTGCTGCCTGTGCTGCTGTCCATCCTGGGCCCGCCGCCAGAGGTG
  ATACAGATGTACAAGGAAAGCCCAGAGATCCTGAGTCCACCAGCTCCACAGGGAGGCGGGCTTAGGTGGG
  GGGCATCCTCCTCCCTGCCCCAGAGCTTTGCCAGAGTGACTACCTCCATGACCGTGGCCATCCACCCACC
  CCCCCTGCCTGGTGCCTACATCCATCCAGCCCCTGATGAGCCCCCTTGGTCCCCTGCTGCCACTAGCTCT
  GGCAACCTCAGTTCCAGGGGACCAGGTCCAGCCACTGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224642 representing NM_003738
  Red=Cloning site Green=Tags(s)
  
  MTRSPPLRELPPSYTPPARTAAPQILAGSLKAPLWLRAYFQGLLFSLGCGIQRHCGKVLFLGLLAFGALA
  LGLRMAIIETNLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTARQEGENILTPEALGLHLQAAL
  TASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPD
  IQWTNLDPEQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLHPDDLHCPPSAPNHHSRQAPNVAHELS
  GGCHGFSHKFMHWQEELLLGGMARDPQGELLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQAST
  VLQAWQRRFVQLAQEALPENASQQIHAFSSTTLDDILHAFSEVSAARVVGGYLLMLAYACVTMLRWDCAQ
  SQGSVGLAGVLLVALAVASGLGLCALLGITFNAATTQVLPFLALGIGVDDVFLLAHAFTEALPGTPLQER
  MGECLQRTGTSVVLTSINNMAAFLMAALVPIPALRAFSLQAAIVVGCTFVAVMLVFPAILSLDLRRRHCQ
  RLDVLCCFSSPCSAQVIQILPQELGDGTVPVGIAHLTATVQAFTHCEASSQHVVTILPPQAHLVPPPSDP
  LGSELFSPGGSTRDLLGQEEETRQKAACKSLPCARWNLAHFARYQFAPLLLQSHAKAIVLVLFGALLGLS
  LYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVL
  PPPATQAPRTWLHYYRNWLQGIQAAFDQDWASGRITRHSYRNGSEDGALAYKLLIQTGDAQEPLDFSQLT
  TRKLVDREGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPPAQPLEFAQF
  PFLLRGLQKTADFVEAIEGARAACAEAGQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLVCTFLVCA
  LLLLNPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASVGIGVEFTVHVALGFLTTQGSRNLR
  AAHALEHTFAPVTDGAISTLLGLLMLAGSHFDFIVRYFFAALTVLTLLGLLHGLVLLPVLLSILGPPPEV
  IQMYKESPEILSPPAPQGGGLRWGASSSLPQSFARVTTSMTVAIHPPPLPGAYIHPAPDEPPWSPAATSS
  GNLSSRGPGPATG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003738
• ORF Size: 3609 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003738.1, NM_003738.2, NM_003738.3, NM_003738.4, NP_003729.3
• RefSeq Size: 3624
• RefSeq ORF: 3612
• Locus ID: 8643
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer
• MW: 130.4 kDa
• Gene Summary: This gene encodes a transmembrane receptor of the patched gene family. The encoded protein may function as a tumor suppressor in the hedgehog signaling pathway. Alterations in this gene have been associated with nevoid basal cell carcinoma syndrome, basal cell carcinoma, medulloblastoma, and susceptibility to congenital macrostomia. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]