IRE1 (ERN2) (NM_033266) Human Tagged ORF Clone

CAT#: RC224780

ERN2 (Myc-DDK-tagged)-Human endoplasmic reticulum to nucleus signaling 2 (ERN2)


  "NM_033266" in other vectors (7)

Reconstitution Protocol

USD 750.00

In Stock*

Size
    • 10 ug

Product Images

Other products for "ERN2"

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol ERN2
Synonyms hIRE2p; IRE1-BETA; IRE1b; IRE2p
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC224780 representing NM_033266
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGAAGGGCGGGGATCGGCGAGGACTCCAGGCTGGGGTTGCAGGCCCAGCCAGGGGCGGAGCCTTCTC
CGGGTCGGGCGGGGACAGAGCGCTCCCTTGGAGGCGCCCAGGGACCTGGCCAGCCGTGCAGCTGCCCAGG
CGCTATGGCGAGTGCGGTCAGGGGGTCGAGGCCGTGGCCCCGGCTGGGGCTCCAGCTCCAGTTCGCGGCG
CTGCTGCTCGGGACGCTGAGTCCACAGGTTCATACTCTCAGGCCAGAGAACCTCCTGCTGGTGTCCACCT
TGGATGGAAGTCTCCACGCACTAAGCAAGCAGACAGGGGACCTGAAGTGGACTCTGAGGGATGATCCCGT
CATCGAAGGACCAATGTACGTCACAGAAATGGCCTTTCTCTCTGACCCAGCAGATGGCAGCCTGTACATC
TTGGGGACCCAAAAACAACAGGGATTAATGAAACTGCCATTCACCATCCCTGAGCTGGTTCATGCCTCTC
CCTGCCGCAGCTCTGATGGGGTCTTCTACACAGGCCGGAAGCAGGATGCCTGGTTTGTGGTGGACCCTGA
GTCAGGGGAGACCCAGATGACACTGACCACAGAGGGTCCCTCCACCCCCCGCCTCTACATTGGCCGAACA
CAGTATACGGTCACCATGCATGACCCAAGAGCCCCAGCCCTGCGCTGGAACACCACCTACCGCCGCTACT
CAGCGCCCCCCATGGATGGCTCACCTGGGAAATACATGAGCCACCTGGCGTCCTGCGGGATGGGCCTGCT
GCTCACTGTGGACCCAGGAAGCGGGACGGTGCTGTGGACACAGGACCTGGGCGTGCCTGTGATGGGCGTC
TACACCTGGCACCAGGACGGCCTGCGCCAGCTGCCGCATCTCACGCTGGCTCGAGACACTCTGCATTTCC
TCGCCCTCCGCTGGGGCCACATCCGACTGCCTGCCTCAGGCCCCCGGGACACAGCCACCCTCTTCTCTAC
CTTGGACACCCAGCTGCTAATGACGCTGTATGTGGGGAAGGATGAAACTGGCTTCTATGTCTCTAAAGCA
CTGGTCCACACAGGAGTGGCCCTGATGCCTCGTGGACTGACCCTGGCCCCCGCAGATGGCCCCACCACAG
ATGAGGTGACACTCCAAGTCTCAGGAGAGCGAGAGGGCTCACCCAGCACTGCTGTTAGATACCCCTCAGG
CAGTGTGGCCCTCCCAAGCCAGTGGCTGCTCATTGGACACCACGAGCTACCCCCAGTCCTGCACACCACC
ATGCTGAGGGTCCATCCCACCCTGGGGAGTGGAACTGCAGAGACAAGACCTCCAGAGAATACCCAGGCCC
CAGCCTTCTTCTTGGAGCTATTGAGCCTGAGCCGAGAGAAACTTTGGGACTCCGAGCTGCATCCAGAAGA
AAAAACTCCAGACTCTTACTTGGGGCTGGGACCCCAAGACCTGCTGGCAGCTAGCCTCACTGCTGTCCTC
CTGGGAGGGTGGATTCTCTTTGTGATGAGGCAGCAACAGCCGCAGGTGGTGGAGAAGCAGCAGGAGACCC
CCCTGGCACCTGCAGACTTTGCTCACATCTCCCAGGATGCCCAGTCCCTGCACTCGGGGGCCAGCCGGAG
GAGCCAGAAGAGGCTTCAGAGTCCCTCAAAGCAAGCCCAGCCACTCGACGACCCTGAAGCTGAGCAACTC
ACCGTAGTGGGGAAGATTTCCTTCAATCCCAAGGACGTGCTGGGCCGCGGGGCAGGCGGGACTTTCGTTT
TCCGGGGACAGTTTGAGGGACGGGCAGTGGCTGTCAAGCGGCTCCTCCGCGAGTGCTTTGGCCTGGTTCG
GCGGGAAGTTCAACTGCTGCAGGAGTCTGACAGGCACCCCAACGTGCTCCGCTACTTCTGCACCGAGCGG
GGACCCCAGTTCCACTACATTGCCCTGGAGCTCTGCCGGGCCTCCTTGCAGGAGTACGTAGAAAACCCGG
ACCTGGATCGCGGGGGTCTGGAGCCCGAGGTCGTGCTGCAGCAGCTGATGTCTGGCCTGGCCCACCTGCA
CTCTTTACACATAGTGCACCGGGACCTGAAGCCAGGAAATATTCTCATCACCGGGCCTGACAGCCAGGGC
CTGGGCAGAGTGGTGCTCTCAGACTTCGGCCTCTGCAAGAAGCTGCCTGCTGGCCGCTGTAGCTTCAGCC
TCCACTCCGGCATCCCCGGCACGGAAGGCTGGATGGCGCCCGAGCTTCTGCAGCTCCTGCCACCAGACAG
TCCTACCAGCGCTGTGGACATCTTCTCTGCAGGCTGCGTGTTCTACTACGTGCTTTCTGGTGGCAGCCAC
CCCTTTGGAGACAGTCTTTATCGCCAGGCAAACATCCTCACAGGGGCTCCCTGTCTGGCTCACCTGGAGG
AAGAGGTCCACGACAAGGTGGTTGCCCGGGACCTGGTTGGAGCCATGTTGAGCCCACTGCCGCAGCCACG
CCCCTCTGCCCCCCAGGTGCTGGCCCACCCCTTCTTTTGGAGCAGAGCCAAGCAACTCCAGTTCTTCCAG
GACGTCAGTGACTGGCTGGAGAAGGAGTCCGAGCAGGAGCCCCTGGTGAGGGCACTGGAGGCGGGAGGCT
GCGCAGTGGTCCGGGACAACTGGCACGAGCACATCTCCATGCCGCTGCAGACAGATCTGAGAAAGTTCCG
GTCCTATAAGGGGACATCAGTGCGAGACCTGCTCCGTGCTGTGAGGAACAAGAAGCACCACTACAGGGAG
CTCCCAGTTGAGGTGCGACAGGCACTCGGCCAAGTCCCTGATGGCTTCGTCCAGTACTTCACAAACCGCT
TCCCACGGCTGCTCCTCCACACGCACCGAGCCATGAGGAGCTGCGCCTCTGAGAGCCTCTTCCTGCCCTA
CTACCCGCCAGACTCAGAGGCCAGGAGGCCATGCCCTGGGGCCACAGGGAGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC224780 representing NM_033266
Red=Cloning site Green=Tags(s)

MRRAGIGEDSRLGLQAQPGAEPSPGRAGTERSLGGAQGPGQPCSCPGAMASAVRGSRPWPRLGLQLQFAA
LLLGTLSPQVHTLRPENLLLVSTLDGSLHALSKQTGDLKWTLRDDPVIEGPMYVTEMAFLSDPADGSLYI
LGTQKQQGLMKLPFTIPELVHASPCRSSDGVFYTGRKQDAWFVVDPESGETQMTLTTEGPSTPRLYIGRT
QYTVTMHDPRAPALRWNTTYRRYSAPPMDGSPGKYMSHLASCGMGLLLTVDPGSGTVLWTQDLGVPVMGV
YTWHQDGLRQLPHLTLARDTLHFLALRWGHIRLPASGPRDTATLFSTLDTQLLMTLYVGKDETGFYVSKA
LVHTGVALMPRGLTLAPADGPTTDEVTLQVSGEREGSPSTAVRYPSGSVALPSQWLLIGHHELPPVLHTT
MLRVHPTLGSGTAETRPPENTQAPAFFLELLSLSREKLWDSELHPEEKTPDSYLGLGPQDLLAASLTAVL
LGGWILFVMRQQQPQVVEKQQETPLAPADFAHISQDAQSLHSGASRRSQKRLQSPSKQAQPLDDPEAEQL
TVVGKISFNPKDVLGRGAGGTFVFRGQFEGRAVAVKRLLRECFGLVRREVQLLQESDRHPNVLRYFCTER
GPQFHYIALELCRASLQEYVENPDLDRGGLEPEVVLQQLMSGLAHLHSLHIVHRDLKPGNILITGPDSQG
LGRVVLSDFGLCKKLPAGRCSFSLHSGIPGTEGWMAPELLQLLPPDSPTSAVDIFSAGCVFYYVLSGGSH
PFGDSLYRQANILTGAPCLAHLEEEVHDKVVARDLVGAMLSPLPQPRPSAPQVLAHPFFWSRAKQLQFFQ
DVSDWLEKESEQEPLVRALEAGGCAVVRDNWHEHISMPLQTDLRKFRSYKGTSVRDLLRAVRNKKHHYRE
LPVEVRQALGQVPDGFVQYFTNRFPRLLLHTHRAMRSCASESLFLPYYPPDSEARRPCPGATGR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_033266
ORF Size 2922 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_033266.2, NP_150296.2
RefSeq Size 3549 bp
RefSeq ORF 2781 bp
Locus ID 10595
Protein Families Druggable Genome, Protein Kinase
MW 107 kDa

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.