KIF1B (NM_183416) Human Tagged ORF Clone

CAT#: RC224955

KIF1B (Myc-DDK-tagged)-Human kinesin family member 1B (KIF1B), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY405200) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224955 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KIF1B
• Synonyms: CMT2; CMT2A; CMT2A1; HMSNII; KLP; NBLST1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224955 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGGAGCCTCAGTGAAGGTGGCTGTCCGGGTAAGGCCCTTCAATTCTCGAGAGACCAGCAAGGAAT
  CCAAATGCATCATTCAGATGCAAGGCAACTCGACCAGTATTATTAACCCAAAGAATCCAAAGGAAGCTCC
  AAAGTCCTTCAGCTTCGACTATTCCTACTGGTCTCATACCTCACCCGAAGATCCCTGTTTTGCATCTCAA
  AACCGTGTGTACAATGACATTGGCAAGGAAATGCTCTTACACGCCTTTGAGGGATATAATGTCTGTATTT
  TTGCCTATGGGCAGACTGGTGCTGGAAAATCTTATACAATGATGGGTAAACAAGAAGAAAGCCAGGCTGG
  CATCATTCCACAGTTATGTGAAGAACTTTTTGAGAAAATCAATGACAACTGTAATGAAGAAATGTCTTAC
  TCTGTAGAGGTGAGCTACATGGAAATTTACTGTGAAAGAGTACGAGATTTGCTGAATCCAAAAAACAAGG
  GTAATTTGCGTGTGCGTGAACACCCACTTCTTGGACCCTATGTGGAGGATCTGTCCAAGTTGGCAGTTAC
  TTCCTACACAGACATTGCTGACCTCATGGATGCTGGGAACAAAGCCAGGACAGTGGCAGCTACAAACATG
  AATGAAACAAGTAGCCGTTCCCACGCTGTGTTTACGATTGTTTTCACCCAGAAGAAACACGATAATGAGA
  CCAACCTTTCCACTGAGAAGGTCAGTAAAATCAGCTTGGTGGATCTAGCAGGAAGTGAACGAGCTGATTC
  AACTGGTGCCAAAGGGACTCGATTAAAGGAAGGAGCAAATATTAATAAGTCTCTTACAACTTTGGGCAAA
  GTCATTTCAGCCTTGGCCGAGGTGAGTAAAAAGAAGAAGAAAACAGATTTTATTCCCTACAGGGATTCTG
  TACTTACTTGGCTCCTTCGAGAAAATTTAGGTGGCAATTCTCGGACTGCAATGGTTGCTGCTCTGAGCCC
  CGCGGATATCAACTACGATGAGACTTTGAGCACTCTGAGATATGCAGATCGTGCAAAACAAATTAAATGC
  AATGCTGTTATCAATGAGGACCCCAATGCCAAACTGGTTCGTGAATTAAAGGAGGAGGTGACACGGCTGA
  AGGACCTTCTTCGTGCTCAGGGCCTGGGAGATATTATTGATACATCCATGGGGTCCCTCACTTCATCCCC
  ATCTTCCTGCTCACTCAGTAGTCAGGTGGGCTTGACGTCTGTGACCAGTATTCAAGAGAGGATCATGTCT
  ACACCTGGAGGAGAGGAAGCTATTGAACGTTTAAAGGAATCAGAGAAGATCATTGCTGAGTTGAATGAAA
  CTTGGGAAGAGAAGCTTCGTAAAACAGAGGCCATCAGAATGGAGAGAGAGGCTTTGTTGGCTGAGATGGG
  AGTTGCCATTCGGGAAGATGGAGGAACCCTAGGGGTTTTCTCACCTAAAAAGACCCCACATCTTGTTAAC
  CTCAATGAAGACCCACTAATGTCTGAGTGCCTACTTTATTACATCAAAGATGGAATTACAAGGGTTGGCC
  AAGCAGATGCTGAGCGGCGCCAGGACATAGTGCTGAGCGGGGCTCACATTAAAGAAGAGCATTGTATCTT
  CCGGAGTGAGAGAAGCAACAGCGGGGAAGTTATCGTGACCTTAGAGCCCTGTGAGCGCTCAGAAACCTAC
  GTAAATGGCAAGAGGGTGTCCCAGCCTGTTCAGCTGCGCTCAGGAAACCGTATCATCATGGGTAAAAACC
  ATGTTTTCCGCTTTAACCACCCGGAACAAGCACGAGCTGAGCGAGAGAAGACTCCTTCTGCTGAGACCCC
  CTCTGAGCCTGTGGACTGGACATTTGCCCAGAGGGAGCTTCTGGAAAAACAAGGAATTGATATGAAACAA
  GAGATGGAGAAAAGGCTACAGGAAATGGAGATCTTATACAAAAAGGAGAAGGAAGAAGCAGATCTTCTTT
  TGGAGCAGCAGAGACTGGACGCGGATTCTGATAGCGGGGACGATTCTGACAAGAGGTCGTGTGAAGAGAG
  CTGGAAACTGATTACTTCTCTGAGAGAAAAGCTACCTCCCAGCAAGTTGCAAACCATTGTTAAAAAATGT
  GGCCTCCCAAGCAGTGGGAAGAAACGTGAACCAATTAAAATGTATCAGATACCCCAAAGAAGGCGCTTGA
  GTAAAGATTCCAAGTGGGTCACAATCTCAGATCTTAAAATTCAGGCTGTCAAAGAGATTTGCTATGAGGT
  TGCTCTCAATGACTTCAGGCACAGTCGGCAGGAGATTGAAGCCCTGGCCATTGTCAAGATGAAGGAGCTT
  TGTGCCATGTATGGCAAGAAAGACCCCAATGAGCGGGACTCCTGGAGGGCAGTGGCCAGGGACGTCTGGG
  ATACCGTCGGTGTTGGGGATGAGAAGATCGAAGACGTCATGGCCACTGGGAAAGGCAGCACTGATGTAGA
  TGACCTCAAGGTTCATATAGACAAGCTGGAAGATATTTTGCAAGAAGTCAAAAAGCAAAATAACATGAAA
  GACGAGGAGATAAAAGTCTTAAGAAATAAAATGCTCAAAATGGAAAAAGTCTTGCCACTGATCGGATCTC
  AGGAACAGAAAAGCCCAGGAAGCCACAAAGCAAAGGAGCCTGTTGGTGCTGGTGTTAGTAGCACCTCTGA
  GAATAATGTAAGTAAAGGAGACAATGGAGAACTTGCAAAAGAAGAACGTGTTTCCCAGCTGATGAATGGG
  GATCCAGCTTTTAGACGTGGACGTCTGCGCTGGATGAGGCAAGAGCAAATTCGGTTTAAGAACTTGCAAC
  AGCAGGAGATAACAAAGCAGCTTCGTCGGCAGAATGTACCTCATAGGTTCATCCCTCCTGAGAACCGGAA
  GCCCCGCTTCCCCTTTAAGAGCAACCCTAAACACAGAAACTCTTGGAGTCCTGGGACACATATCATCATA
  ACAGAAGATGAGGTTATAGAGCTTAGGATTCCAAAAGACGATGAAGCAAGGAAAGGGAATAAAGAAGAGA
  GCCAAGAAAAAGGGGGTAAAGGAGCTTTTAAGGATCCCCAGTTTCCATGGGGCTCTCAAGGAATGAGAAG
  TCAAGATCACATCCAAGTTAGCAAGCAGCACATTAATAATCAGCAACAGCCACCTCAACTACGTTGGAGA
  AGCAATTCTCTCAATAATGGCCAGCCGAAAAGTACGCGCTGCCAGGCATCTGCCTCCGCGGAGTCATTAA
  ACTCCCACAGTGGTCACCCCACTGCTGATGTACAGACTTTCCAGGCAAAGCGCCATATTCATCAACACCG
  TCAGTCTTACTGTAATTATAACACTGGAGGTCAGTTAGAGGGCAATGCAGCCACTTCCTATCAGAAGCAG
  ACTGACAAACCCAGCCACTGTAGCCAGTTTGTGACACCTCCGCGGATGAGGAGACAGTTCTCAGCACCCA
  ATCTCAAAGCTGGTCGAGAAACCACAGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224955 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSGASVKVAVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWSHTSPEDPCFASQ
  NRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMMGKQEESQAGIIPQLCEELFEKINDNCNEEMSY
  SVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAATNM
  NETSSRSHAVFTIVFTQKKHDNETNLSTEKVSKISLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGK
  VISALAEVSKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKC
  NAVINEDPNAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLSSQVGLTSVTSIQERIMS
  TPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRMEREALLAEMGVAIREDGGTLGVFSPKKTPHLVN
  LNEDPLMSECLLYYIKDGITRVGQADAERRQDIVLSGAHIKEEHCIFRSERSNSGEVIVTLEPCERSETY
  VNGKRVSQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPSEPVDWTFAQRELLEKQGIDMKQ
  EMEKRLQEMEILYKKEKEEADLLLEQQRLDADSDSGDDSDKRSCEESWKLITSLREKLPPSKLQTIVKKC
  GLPSSGKKREPIKMYQIPQRRRLSKDSKWVTISDLKIQAVKEICYEVALNDFRHSRQEIEALAIVKMKEL
  CAMYGKKDPNERDSWRAVARDVWDTVGVGDEKIEDVMATGKGSTDVDDLKVHIDKLEDILQEVKKQNNMK
  DEEIKVLRNKMLKMEKVLPLIGSQEQKSPGSHKAKEPVGAGVSSTSENNVSKGDNGELAKEERVSQLMNG
  DPAFRRGRLRWMRQEQIRFKNLQQQEITKQLRRQNVPHRFIPPENRKPRFPFKSNPKHRNSWSPGTHIII
  TEDEVIELRIPKDDEARKGNKEESQEKGGKGAFKDPQFPWGSQGMRSQDHIQVSKQHINNQQQPPQLRWR
  SNSLNNGQPKSTRCQASASAESLNSHSGHPTADVQTFQAKRHIHQHRQSYCNYNTGGQLEGNAATSYQKQ
  TDKPSHCSQFVTPPRMRRQFSAPNLKAGRETTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183416
• ORF Size: 3459 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183416.2, NM_183416.3, NP_904325.2
• RefSeq Size: 7680 bp
• RefSeq ORF: 3462 bp
• Locus ID: 23095
• Protein Families: Druggable Genome
• MW: 130.4 kDa
• Gene Summary: This gene encodes a motor protein that transports mitochondria and synaptic vesicle precursors. Mutations in this gene cause Charcot-Marie-Tooth disease, type 2A1. [provided by RefSeq, Jul 2008]