Insulin Receptor R (INSRR) (NM_014215) Human Tagged ORF Clone

CAT#: RC224956

INSRR (Myc-DDK-tagged)-Human insulin receptor-related receptor (INSRR)

Product Images

Western blot validation of overexpression lysate (Cat# LY415430) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC224956 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified INSRR protein (Cat# TP324956). The protein was produced from HEK293T cells transfected with INSRR cDNA clone (Cat# RC224956) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: INSRR
• Synonyms: IRR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224956 representing NM_014215
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGTGCCTAGTCTGTGGCCCTGGGGAGCATGCCTGCCTGTGATCTTCCTCTCCTTGGGATTTGGCC
  TGGATACAGTAGAGGTGTGCCCCAGCCTGGATATTCGCTCAGAGGTGGCAGAGCTTCGTCAGCTGGAGAA
  CTGCAGCGTGGTGGAGGGCCACCTGCAGATCCTGCTCATGTTCACAGCCACCGGGGAGGACTTCCGCGGC
  CTCAGCTTCCCTCGCCTCACCCAGGTCACCGACTACCTGCTGCTCTTCCGTGTCTACGGACTGGAGAGCC
  TGCGCGACCTCTTCCCCAACCTAGCAGTCATCCGCGGGACGCGCCTCTTCCTGGGCTATGCACTGGTCAT
  CTTTGAGATGCCACATCTGCGTGACGTGGCACTGCCTGCACTTGGGGCCGTGCTGCGTGGGGCTGTGCGT
  GTGGAGAAGAACCAGGAGCTCTGCCACCTCTCCACCATTGACTGGGGACTGCTGCAGCCAGCACCTGGCG
  CCAACCACATCGTGGGCAACAAGCTGGGCGAGGAGTGTGCTGACGTGTGCCCTGGTGTGCTGGGTGCTGC
  TGGTGAGCCCTGTGCCAAGACCACCTTCAGCGGGCACACTGACTACAGATGCTGGACCTCCAGCCACTGC
  CAGAGAGTGTGCCCCTGCCCCCATGGGATGGCTTGCACAGCGAGGGGCGAGTGCTGCCACACCGAATGCC
  TGGGGGGCTGCAGCCAGCCAGAAGACCCTCGTGCCTGTGTAGCTTGCCGCCACCTCTACTTCCAGGGTGC
  CTGCCTGTGGGCCTGCCCGCCAGGCACCTACCAGTATGAGTCCTGGCGCTGTGTCACAGCTGAGCGCTGT
  GCCAGCCTGCACTCTGTGCCCGGCCGTGCCTCCACCTTCGGCATACACCAGGGCAGTTGCCTGGCCCAGT
  GCCCTTCTGGCTTCACCCGTAATAGCAGCAGCATATTCTGCCACAAGTGCGAGGGGCTGTGCCCTAAAGA
  GTGCAAGGTAGGCACCAAGACCATCGACTCCATCCAGGCGGCACAGGATCTTGTGGGCTGCACGCATGTG
  GAGGGAAGCCTCATCCTCAACCTTCGCCAGGGCTACAACCTGGAGCCACAGCTGCAGCACAGCCTGGGGC
  TGGTAGAAACCATTACTGGCTTCCTCAAAATCAAGCACTCCTTTGCCCTCGTGTCCCTGGGCTTTTTCAA
  GAACCTCAAACTAATCCGGGGAGACGCCATGGTGGATGGGAACTACACTCTCTACGTGCTGGACAACCAG
  AACCTACAACAGCTAGGGTCCTGGGTGGCCGCGGGGCTCACCATTCCCGTGGGCAAGATCTACTTCGCCT
  TCAACCCGCGCCTCTGCTTGGAACACATCTACCGACTGGAGGAGGTGACAGGCACGCGAGGTCGGCAGAA
  CAAGGCTGAGATCAACCCCCGCACCAACGGAGACCGCGCCGCCTGCCAGACTCGCACCCTGCGCTTCGTG
  TCCAACGTGACGGAGGCAGACCGCATCCTGCTACGCTGGGAGCGCTATGAGCCACTGGAGGCCCGCGACC
  TGCTCAGCTTCATCGTGTACTACAAGGAGTCCCCATTCCAGAACGCCACAGAGCACGTGGGTCCAGATGC
  TTGTGGAACCCAGAGCTGGAACCTGCTGGATGTGGAGCTGCCCCTAAGCCGCACCCAGGAGCCAGGGGTG
  ACCCTAGCCTCCCTCAAGCCTTGGACACAGTACGCAGTGTTTGTGCGGGCCATCACGCTAACCACTGAGG
  AGGACAGCCCTCATCAAGGAGCCCAGAGTCCCATCGTCTACCTCCGAACGCTGCCTGCAGCTCCCACGGT
  GCCCCAAGACGTCATCTCCACGTCCAACTCCTCCTCCCACCTCCTGGTGCGCTGGAAGCCACCGACCCAG
  CGCAATGGGAACCTCACCTACTACCTGGTGCTGTGGCAGCGGCTGGCAGAGGACGGCGACCTCTACCTCA
  ATGACTACTGCCACCGCGGCTTGCGGCTGCCCACCAGCAACAACGATCCGCGCTTCGACGGCGAAGACGG
  GGATCCTGAGGCCGAGATGGAGTCCGACTGCTGCCCTTGCCAGCACCCACCTCCTGGTCAGGTTCTGCCC
  CCGCTGGAGGCGCAAGAGGCCTCGTTCCAGAAGAAGTTTGAAAACTTTCTACACAACGCGATCACCATCC
  CCATATCCCCTTGGAAGGTGACGTCCATCAACAAGAGCCCCCAAAGGGACTCAGGGCGGCACCGCCGGGC
  AGCTGGGCCCCTCCGGCTGGGGGGCAACAGCTCGGATTTCGAGATCCAGGAGGACAAGGTGCCCCGTGAG
  CGAGCGGTGCTGAGCGGCCTGCGCCACTTCACGGAATACCGGATCGACATCCATGCCTGCAACCACGCGG
  CGCACACCGTGGGCTGCAGCGCCGCCACCTTCGTCTTTGCGCGCACCATGCCCCACAGAGAGGCTGATGG
  TATTCCAGGAAAGGTGGCCTGGGAGGCCTCCAGCAAGAACAGTGTCCTTCTGCGCTGGCTCGAGCCACCA
  GACCCCAACGGACTCATCCTCAAGTACGAAATCAAGTACCGCCGCTTGGGAGAGGAGGCCACAGTGCTGT
  GTGTGTCCCGTCTTCGATATGCGAAGTTTGGGGGAGTCCACCTGGCCCTGCTGCCCCCTGGAAACTACTC
  TGCCAGGGTTAGGGCAACCTCACTGGCTGGCAATGGCTCTTGGACAGACAGTGTTGCCTTCTACATCCTT
  GGCCCAGAGGAGGAGGATGCTGGGGGGCTGCATGTCCTCCTCACTGCCACCCCTGTGGGGCTCACGCTGC
  TCATCGTTCTTGCTGCCCTTGGTTTCTTCTACGGCAAGAAGAGAAACAGAACCCTGTATGCTTCTGTGAA
  TCCAGAGTACTTCAGCGCCTCTGATATGTATGTCCCTGATGAATGGGAGGTGCCTCGGGAGCAGATCTCG
  ATAATCCGGGAACTGGGCCAGGGCTCTTTTGGGATGGTATATGAGGGGCTGGCACGAGGACTTGAGGCTG
  GAGAGGAGTCCACACCCGTGGCCCTGAAGACGGTGAATGAGCTGGCCAGCCCACGGGAATGCATTGAGTT
  CCTCAAGGAAGCTTCTGTCATGAAAGCCTTCAAGTGTCACCATGTGGTGCGTCTCCTGGGTGTGGTATCT
  CAGGGCCAGCCAACTCTGGTCATCATGGAGTTAATGACCCGTGGGGACCTCAAGAGCCATCTTCGATCTT
  TGCGGCCTGAGGCAGAGAACAACCCTGGGCTCCCACAGCCAGCATTGGGGGAAATGATCCAAATGGCTGG
  TGAGATTGCAGACGGCATGGCCTACCTTGCTGCCAACAAGTTTGTGCACCGAGATCTAGCAGCCCGCAAC
  TGCATGGTGTCCCAGGACTTCACCGTCAAGATCGGGGACTTCGGGATGACTCGGGACGTGTATGAGACAG
  ACTATTACCGCAAGGGTGGGAAGGGGCTGCTGCCCGTGCGCTGGATGGCCCCCGAGTCCCTCAAAGATGG
  GATCTTCACCACCCACTCGGATGTCTGGTCCTTTGGCGTGGTACTCTGGGAGATTGTGACCCTGGCAGAA
  CAACCCTACCAGGGCCTGTCCAATGAGCAGGTGCTGAAGTTCGTCATGGATGGCGGGGTCCTGGAGGAGC
  TGGAGGGCTGTCCCCTTCAGCTGCAGGAGCTGATGAGCCGCTGCTGGCAGCCGAACCCACGCCTGCGCCC
  ATCTTTCACACACATTCTGGACAGCATACAGGAGGAGCTGCGGCCCTCCTTCCGCCTCCTCTCCTTCTAC
  TACAGCCCGGAATGCCGGGGGGCCCGGGGCTCCCTGCCTACCACCGATGCAGAGCCTGACTCCTCACCCA
  CTCCAAGAGACTGCAGCCCTCAAAATGGGGGTCCAGGGCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224956 representing NM_014215
  Red=Cloning site Green=Tags(s)
  
  MAVPSLWPWGACLPVIFLSLGFGLDTVEVCPSLDIRSEVAELRQLENCSVVEGHLQILLMFTATGEDFRG
  LSFPRLTQVTDYLLLFRVYGLESLRDLFPNLAVIRGTRLFLGYALVIFEMPHLRDVALPALGAVLRGAVR
  VEKNQELCHLSTIDWGLLQPAPGANHIVGNKLGEECADVCPGVLGAAGEPCAKTTFSGHTDYRCWTSSHC
  QRVCPCPHGMACTARGECCHTECLGGCSQPEDPRACVACRHLYFQGACLWACPPGTYQYESWRCVTAERC
  ASLHSVPGRASTFGIHQGSCLAQCPSGFTRNSSSIFCHKCEGLCPKECKVGTKTIDSIQAAQDLVGCTHV
  EGSLILNLRQGYNLEPQLQHSLGLVETITGFLKIKHSFALVSLGFFKNLKLIRGDAMVDGNYTLYVLDNQ
  NLQQLGSWVAAGLTIPVGKIYFAFNPRLCLEHIYRLEEVTGTRGRQNKAEINPRTNGDRAACQTRTLRFV
  SNVTEADRILLRWERYEPLEARDLLSFIVYYKESPFQNATEHVGPDACGTQSWNLLDVELPLSRTQEPGV
  TLASLKPWTQYAVFVRAITLTTEEDSPHQGAQSPIVYLRTLPAAPTVPQDVISTSNSSSHLLVRWKPPTQ
  RNGNLTYYLVLWQRLAEDGDLYLNDYCHRGLRLPTSNNDPRFDGEDGDPEAEMESDCCPCQHPPPGQVLP
  PLEAQEASFQKKFENFLHNAITIPISPWKVTSINKSPQRDSGRHRRAAGPLRLGGNSSDFEIQEDKVPRE
  RAVLSGLRHFTEYRIDIHACNHAAHTVGCSAATFVFARTMPHREADGIPGKVAWEASSKNSVLLRWLEPP
  DPNGLILKYEIKYRRLGEEATVLCVSRLRYAKFGGVHLALLPPGNYSARVRATSLAGNGSWTDSVAFYIL
  GPEEEDAGGLHVLLTATPVGLTLLIVLAALGFFYGKKRNRTLYASVNPEYFSASDMYVPDEWEVPREQIS
  IIRELGQGSFGMVYEGLARGLEAGEESTPVALKTVNELASPRECIEFLKEASVMKAFKCHHVVRLLGVVS
  QGQPTLVIMELMTRGDLKSHLRSLRPEAENNPGLPQPALGEMIQMAGEIADGMAYLAANKFVHRDLAARN
  CMVSQDFTVKIGDFGMTRDVYETDYYRKGGKGLLPVRWMAPESLKDGIFTTHSDVWSFGVVLWEIVTLAE
  QPYQGLSNEQVLKFVMDGGVLEELEGCPLQLQELMSRCWQPNPRLRPSFTHILDSIQEELRPSFRLLSFY
  YSPECRGARGSLPTTDAEPDSSPTPRDCSPQNGGPGH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014215
• ORF Size: 3891 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014215.1, NM_014215.2, NP_055030.1
• RefSeq Size: 4148 bp
• RefSeq ORF: 3894 bp
• Locus ID: 3645
• Cytogenetics: 1q23.1
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Prostate cancer, Regulation of actin cytoskeleton
• MW: 143.72 kDa
• Gene Summary: ''