Myeloid leukemia factor 1 (MLF1) (NM_001130156) Human Tagged ORF Clone
CAT#: RC225308
- TrueORF®
MLF1 (Myc-DDK-tagged)-Human myeloid leukemia factor 1 (MLF1), transcript variant 3
"NM_001130156" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | MLF1 |
Synonyms | myeloid leukemia factor 1; myeloid leukemia factor 1 variant 1; myeloid leukemia factor 1 variant 2; myeloid leukemia factor 1 variant 3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC225308 representing NM_001130156
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCAGGATGCTGAACAGCAGTTTTGAGGATGACCCCTTCTTCTCTGAGTCCATTCTTGCACACCGAG AAAATATGCGACAGATGATAAGAAGTTTTTCTGAACCCTTTGGAAGAGACTTGCTCAGTATCTCTGATGG TAGAGGGAGAGCTCATAATCGTAGAGGACATAATGATGGTGAAGATTCTTTGACTCATACAGATGTCAGC TCTTTCCAGACAATGGACCAAATGGTGTCAAATATGAGAAACTATATGCAGAAATTAGAAAGAAACTTCG GTCAACTTTCAGTGGATCCAAATGGACATTCATTTTGTTCTTCCTCAGTTATGACTTATTCCAAAATAGG AGATGAACCGCCAAAGGTTTTTCAGGCCTCAACTCAAACTCGTCGAGCTCCAGGAGGAATAAAGGAAACC AGGAAAGCAATGAGAGATTCTGACAGTGGACTAGAAAAAATGGCTATTGGTCATCATATCCATGACCGAG CTCATGTCATTAAAAAGTCAAAGAACAAGAAGACTGGAGATGAAGAGGTCAACCAGGAGTTCATCAATAT GAATGAAAGTGATGCTCATGCTTTTGATGAGGAGTGGCAAAGTGAGGTTTTGAAGTACAAACCAGGACGA CACAATCTAGGAAACACTAGAATGAGAAGTGTTGGCCATGAGAATCCTGGCTCCCGAGAACTTAAAAGAA GGGAGAAACCTCAACAAAGTCCAGCCATTGAACATGGAAGGAGATCAAATGTTTTGGGGGACAAACTCCA CATCAAAGGCTCATCTGTGAAAAGCAACAAAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225308 representing NM_001130156
Red=Cloning site Green=Tags(s) MFRMLNSSFEDDPFFSESILAHRENMRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTHTDVS SFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKET RKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGR HNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001130156 |
ORF Size | 807 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001130156.1, NM_001130156.2, NP_001123628.1 |
RefSeq Size | 2263 bp |
RefSeq ORF | 732 bp |
Locus ID | 4291 |
Cytogenetics | 3q25.32 |
Protein Families | Druggable Genome |
MW | 30.6 kDa |
Gene Summary | 'This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC324825 | MLF1 (untagged)-Human myeloid leukemia factor 1 (MLF1), transcript variant 3 |
USD 420.00 |
|
RG225308 | MLF1 (GFP-tagged) - Human myeloid leukemia factor 1 (MLF1), transcript variant 3 |
USD 460.00 |
|
RC225308L3 | Lenti-ORF clone of MLF1 (Myc-DDK-tagged)-Human myeloid leukemia factor 1 (MLF1), transcript variant 3 |
USD 620.00 |
|
RC225308L4 | Lenti-ORF clone of MLF1 (mGFP-tagged)-Human myeloid leukemia factor 1 (MLF1), transcript variant 3 |
USD 620.00 |
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