Carbonic Anhydrase I (CA1) (NM_001128829) Human Tagged ORF Clone
CAT#: RC225348L4
- LentiORF®
Lenti-ORF clone of CA1 (mGFP-tagged)-Human carbonic anhydrase I (CA1), transcript variant 1
"NM_001128829" in other vectors (4)
Product Images
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | CA1 |
Synonyms | CA-I; CAB; Car1; HEL-S-11 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC225348).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_001128829 |
ORF Size | 783 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001128829.1, NP_001122301.1 |
RefSeq ORF | 786 bp |
Locus ID | 759 |
Cytogenetics | 8q21.2 |
Protein Families | Druggable Genome |
Protein Pathways | Nitrogen metabolism |
MW | 28.7 kDa |
Gene Summary | 'Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Nov 2016]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC322929 | CA1 (untagged)-Human carbonic anhydrase I (CA1), transcript variant 1 |
USD 420.00 |
|
RC225348 | CA1 (Myc-DDK-tagged)-Human carbonic anhydrase I (CA1), transcript variant 1 |
USD 420.00 |
|
RG225348 | CA1 (GFP-tagged) - Human carbonic anhydrase I (CA1), transcript variant 1 |
USD 460.00 |
|
RC225348L3 | Lenti-ORF clone of CA1 (Myc-DDK-tagged)-Human carbonic anhydrase I (CA1), transcript variant 1 |
USD 620.00 |
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