FANCL (NM_001114636) Human Tagged ORF Clone

CAT#: RC225580

FANCL (Myc-DDK-tagged)-Human Fanconi anemia, complementation group L (FANCL), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY426502) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225580 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FANCL
• Synonyms: FAAP43; PHF9; POG
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225580 representing NM_001114636
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGTGACGGAAGCGAGCCTGTTGCGCCAGTGCCCCCTGCTTCTGCCCCAGAACCGGTCGAAAACCG
  TGTATGAGGGATTCATCTCGGCTCAGGGAAGAGACTTCCACCTTAGGATAGTGTTGCCTGAAGATTTACA
  ACTGAAGAATGCAAGATTATTATGTAGTTGGCAGCTGAGAACAATACTTAGTGGATACCATCGAATAGTA
  CAACAGAGAATGCAGCACTCTCCTGATCTAATGAGCTTTATGATGGAGTTGAAGATGCTTTTGGAAGTTG
  CCTTAAAGAATAGACAAGAGCTGTATGCACTACCTCCTCCTCCCCAGTTCTACTCAAGCCTTATTGAAGA
  GATAGGAACTCTTGGTTGGGATAAACTTGTGTATGCGGATACCTGCTTCAGTACCATCAAGTTAAAAGCA
  GAAGATGCTTCTGGTAGAGAGCATTTAATCACTCTCAAGTTGAAGGCAAAGTATCCTGCAGAATCACCAG
  ATTATTTTGTGGATTTTCCTGTTCCATTTTGTGCCTCCTGGACACCTCAGGTAAATTCTCCTCAGAGCTC
  CTTAATAAGCATTTATAGTCAGTTTTTGGCAGCAATAGAATCACTAAAGGCATTCTGGGATGTTATGGAT
  GAAATCGATGAGAAGACCTGGGTACTTGAGCCAGAAAAACCTCCACGGAGTGCAACAGCACGCAGAATTG
  CATTAGGTAATAATGTTTCCATAAATATAGAGGTAGACCCCAGGCATCCTACTATGCTTCCTGAGTGCTT
  CTTTCTTGGAGCTGACCATGTGGTAAAACCCCTGGGAATTAAGCTGAGCAGGAACATACATTTGTGGGAT
  CCAGAAAATAGTGTGTTACAAAATTTGAAAGATGTTTTAGAAATTGATTTTCCAGCTCGTGCTATCCTGG
  AAAAATCTGATTTTACTATGGATTGTGGAATTTGTTATGCTTATCAACTTGACGGTACCATTCCTGATCA
  AGTGTGTGATAATTCTCAGTGTGGACAACCTTTCCATCAAATATGCTTATATGAGTGGCTGAGAGGACTA
  CTAACTAGTAGACAGAGTTTTAACATCATATTTGGTGAATGTCCATATTGTAGTAAGCCAATTACCTTAA
  AAATGTCTGGAAGGAAACAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225580 representing NM_001114636
  Red=Cloning site Green=Tags(s)
  
  MAVTEASLLRQCPLLLPQNRSKTVYEGFISAQGRDFHLRIVLPEDLQLKNARLLCSWQLRTILSGYHRIV
  QQRMQHSPDLMSFMMELKMLLEVALKNRQELYALPPPPQFYSSLIEEIGTLGWDKLVYADTCFSTIKLKA
  EDASGREHLITLKLKAKYPAESPDYFVDFPVPFCASWTPQVNSPQSSLISIYSQFLAAIESLKAFWDVMD
  EIDEKTWVLEPEKPPRSATARRIALGNNVSINIEVDPRHPTMLPECFFLGADHVVKPLGIKLSRNIHLWD
  PENSVLQNLKDVLEIDFPARAILEKSDFTMDCGICYAYQLDGTIPDQVCDNSQCGQPFHQICLYEWLRGL
  LTSRQSFNIIFGECPYCSKPITLKMSGRKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001114636
• ORF Size: 1140 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001114636.1, NP_001108108.1
• RefSeq ORF: 1143
• Locus ID: 55120
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 43.2 kDa
• Gene Summary: This gene encodes a ubiquitin ligase that is a member of the Fanconi anemia complementation group (FANC). Members of this group are related by their assembly into a common nuclear protein complex rather than by sequence similarity. This gene encodes the protein for complementation group L that mediates monoubiquitination of FANCD2 as well as FANCI. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2018]