INPP1 (NM_001128928) Human Tagged ORF Clone

CAT#: RC225623

INPP1 (Myc-DDK-tagged)-Human inositol polyphosphate-1-phosphatase (INPP1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY427029) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC225623 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: INPP1
• Synonyms: MGC110984
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225623 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGATATCCTCCGGGAGCTGCTCTGTGTCTCTGAGAAGGCTGCTAACATTGCCCGGGCGTGCAGAC
  AGCAGGAAGCCCTCTTCCAGCTGCTGATCGAAGAAAAGAAAGAGGGAGAAAAGAACAAGAAGTTTGCAGT
  TGACTTCAAGACGCTGGCTGATGTACTGGTACAGGAAGTTATAAAACAGAATATGGAGAACAAGTTTCCA
  GGCTTGGAAAAAAATATTTTTGGAGAAGAATCCAATGAGTTTACTAATGACTGGGGGGAAAAGATTACCT
  TGAGGTTGTGTTCAACAGAGGAGGAAACAGCAGAGCTTCTTAGCAAAGTCCTCAATGGTAACAAGGTGGC
  ATCTGAAGCATTAGCCAGGGTTGTTCATCAGGATGTTGCCTTTACTGACCCAACTCTGGATTCCACAGAG
  ATCAATGTTCCACAGGACATTTTGGGAATTTGGGTGGACCCCATAGATTCAACTTATCAGTATATAAAAG
  GTTCTGCTGACATTAAATCCAACCAGGGAATCTTCCCCTGTGGACTTCAGTGTGTCACCATTTTAATTGG
  TGTCTATGACATACAGACAGGGGTTCCCCTGATGGGAGTCATCAATCAACCTTTTGTGTCACGAGATCCA
  AACACCCTCAGGTGGAAAGGACAGTGCTATTGGGGCCTTTCTTACATGGGGACCAACATGCATTCACTAC
  AGCTCACCATCTCTAGAAGAAACGGCAGTGAAACACACACTGGAAACACCGGCTCTGAGGCAGCATTCTC
  CCCCAGTTTTTCAGCCGTAATTAGTACAAGTGAAAAGGAGACTATCAAAGCTGCATTGTCACGTGTGTGT
  GGAGATCGCATATTTGGGGCAGCTGGGGCTGGTTATAAGAGCCTATGTGTTGTCCAAGGCCTCGTTGACA
  TTTACATCTTTTCAGAAGATACCACATTCAAATGGGACTCTTGTGCTGCTCATGCCATACTGAGGGCCAT
  GGGTGGGGGAATAGTAGACTTGAAAGAATGCTTAGAAAGAAATCCAGAAACAGGGCTTGATTTGCCACAG
  TTGGTGTACCACGTGGAAAATGAGGGTGCTGCTGGGGTGGATCGGTGGGCCAACAAGGGAGGACTCATTG
  CATACAGATCCAGGAAGCGGCTGGAGACATTCCTGAGCCTCCTGGTCCAAAACCTGGCACCTGCAGAGAC
  GCATACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225623 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSDILRELLCVSEKAANIARACRQQEALFQLLIEEKKEGEKNKKFAVDFKTLADVLVQEVIKQNMENKFP
  GLEKNIFGEESNEFTNDWGEKITLRLCSTEEETAELLSKVLNGNKVASEALARVVHQDVAFTDPTLDSTE
  INVPQDILGIWVDPIDSTYQYIKGSADIKSNQGIFPCGLQCVTILIGVYDIQTGVPLMGVINQPFVSRDP
  NTLRWKGQCYWGLSYMGTNMHSLQLTISRRNGSETHTGNTGSEAAFSPSFSAVISTSEKETIKAALSRVC
  GDRIFGAAGAGYKSLCVVQGLVDIYIFSEDTTFKWDSCAAHAILRAMGGGIVDLKECLERNPETGLDLPQ
  LVYHVENEGAAGVDRWANKGGLIAYRSRKRLETFLSLLVQNLAPAETHT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001128928
• ORF Size: 1197 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128928.1, NP_001122400.1
• RefSeq Size: 2179 bp
• RefSeq ORF: 1200 bp
• Locus ID: 3628
• Cytogenetics: 2q32.2
• Protein Families: Druggable Genome
• Protein Pathways: Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 44 kDa
• Gene Summary: 'This gene encodes the enzyme inositol polyphosphate-1-phosphatase, one of the enzymes involved in phosphatidylinositol signaling pathways. This enzyme removes the phosphate group at position 1 of the inositol ring from the polyphosphates inositol 1,4-bisphosphate and inositol 1,3,4-trisphophosphate. [provided by RefSeq, Jul 2008]'