Calcineurin A (PPP3CA) (NM_001130692) Human Tagged ORF Clone

CAT#: RC225795

PPP3CA (Myc-DDK-tagged)-Human protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PPP3CA
• Synonyms: ACCIID; CALN; CALNA; CALNA1; CCN1; CNA1; IECEE; IECEE1; PPP2B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC225795 representing NM_001130692
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGAGCCCAAGGCAATTGATCCCAAGTTGTCGACGACCGACAGGGTGGTGAAAGCTGTTCCATTTC
  CTCCAAGTCACCGGCTTACAGCAAAAGAAGTGTTTGATAATGATGGAAAACCTCGTGTGGATATCTTAAA
  GGCGCATCTTATGAAGGAGGGAAGGCTGGAAGAGAGTGTTGCATTGAGAATAATAACAGAGGGTGCATCA
  ATTCTTCGACAGGAAAAAAATTTGCTGGATATTGATGCGCCAGTCACTGTTTGTGGGGACATTCATGGAC
  AATTCTTTGATTTGATGAAGCTCTTTGAAGTCGGGGGATCTCCTGCCAACACTCGCTACCTCTTCTTAGG
  GGACTATGTTGACAGAGGGTACTTCAGTATTGAATGTGTGCTGTATTTGTGGGCCTTGAAAATTCTCTAC
  CCCAAAACACTGTTTTTACTTCGTGGAAATCATGAATGTAGACATCTAACAGAGTATTTCACATTTAAAC
  AAGAATGTAAAATAAAGTATTCAGAACGCGTATATGATGCCTGTATGGATGCCTTTGACTGCCTTCCCCT
  GGCTGCCCTGATGAACCAACAGTTCCTGTGTGTGCATGGTGGTTTGTCTCCAGAGATTAACACTTTAGAT
  GATATCAGAAAATTAGACCGATTCAAAGAACCACCTGCATATGGACCTATGTGTGATATCCTGTGGTCAG
  ACCCCCTGGAAGATTTTGGAAATGAGAAGACTCAGGAACATTTCACTCACAACACAGTCAGGGGGTGTTC
  ATACTTCTACAGTTACCCGGCTGTATGTGAATTCTTACAGCACAATAACTTGTTATCTATACTCCGAGCC
  CACGAAGCCCAAGATGCAGGGTACCGCATGTACAGGAAAAGCCAAACAACAGGCTTCCCTTCTCTAATTA
  CAATTTTTTCAGCACCAAATTACTTAGATGTATACAATAACAAAGTGACTGAGATGCTGGTAAATGTCCT
  CAACATCTGCTCAGATGATGAACTAGGGTCAGAAGAAGATGGATTTGATGGTGCAACAGCTGCAGCCCGG
  AAAGAGGTGATAAGGAACAAGATCCGAGCAATAGGCAAAATGGCCAGAGTGTTCTCAGTGCTCAGAGAAG
  AGAGTGAGAGTGTGCTGACGCTGAAAGGCTTGACCCCAACTGGCATGCTCCCCAGCGGAGTACTTTCTGG
  AGGGAAGCAAACCCTGCAAAGCGCTATCAAAGGATTTTCACCACAACATAAGATCACTAGCTTCGAGGAA
  GCCAAGGGCTTAGACCGAATTAATGAGAGGATGCCGCCTCGCAGAGATGCCATGCCCTCTGACGCCAACC
  TTAACTCCATCAACAAGGCTCTCACCTCAGAGACTAACGGCACGGACAGCAATGGCAGTAATAGCAGCAA
  TATTCAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC225795 representing NM_001130692
  Red=Cloning site Green=Tags(s)
  
  MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEESVALRIITEGAS
  ILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILY
  PKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLD
  DIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRA
  HEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKVTEMLVNVLNICSDDELGSEEDGFDGATAAAR
  KEVIRNKIRAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSAIKGFSPQHKITSFEE
  AKGLDRINERMPPRRDAMPSDANLNSINKALTSETNGTDSNGSNSSNIQ
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001130692
• ORF Size: 1407 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001130692.1, NP_001124164.1
• RefSeq ORF: 1410 bp
• Locus ID: 5530
• Cytogenetics: 4q24
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Axon guidance, B cell receptor signaling pathway, Calcium signaling pathway, Long-term potentiation, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Oocyte meiosis, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• MW: 52.5 kDa
• Gene Summary: ''