ABAT (NM_001127448) Human Tagged ORF Clone
CAT#: RC225860
ABAT (Myc-DDK-tagged)-Human 4-aminobutyrate aminotransferase (ABAT), transcript variant 3
"NM_001127448" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ABAT |
Synonyms | GABA-AT; GABAT; NPD009 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC225860 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCCATGTTGCTCGCCCAGCGCCTGGCCTGCAGCTTCCAGCACAGCTACCGCCTGCTGGTGCCTG GATCCAGACACATTAGTCAAGCTGCAGCCAAAGTCGACGTTGAATTTGATTATGATGGGCCTCTGATGAA GACGGAAGTCCCAGGGCCTAGATCTCGGGAGTTAATGAAACAGCTGAATATAATTCAGAATGCAGAGGCT GTGCATTTTTTCTGCAATTACGAAGAGAGCCGAGGCAATTACCTGGTTGATGTGGACGGCAACCGAATGC TGGATCTTTATTCCCAGATCTCCTCTGTCCCCATAGGTTACAGCCACCCCGCCCTGCTGAAACTCATCCA ACAGCCTCAAAATGCGAGCATGTTTGTCAACAGACCCGCCCTCGGAATCCTGCCTCCGGAGAACTTTGTG GAGAAGCTCCGGCAGTCCTTGCTCTCGGTGGCTCCCAAAGGGATGTCCCAGCTCATCACCATGGCCTGCG GCTCCTGCTCCAATGAAAACGCCTTAAAGACCATCTTCATGTGGTACCGGAGCAAGGAAAGAGGGCAGAG GGGCTTCTCCCAGGAGGAGCTGGAGACGTGCATGATTAACCAGGCCCCTGGCTGCCCCGACTACAGCATC CTCTCCTTCATGGGCGCGTTCCATGGGAGGACCATGGGTTGCTTAGCGACCACGCACTCTAAAGCCATTC ACAAGATCGACATCCCTTCCTTTGACTGGCCCATCGCACCGTTCCCACGGCTGAAATACCCTCTGGAAGA GTTTGTGAAAGAGAACCAACAGGAGGAGGCCCGCTGTCTGGAAGAGGTGGAGGATCTGATTGTGAAATAT CGGAAAAAGAAGAAGACGGTGGCCGGGATCATCGTGGAGCCCATCCAGTCCGAGGGTGGAGACAACCACG CATCCGATGACTTCTTTCGGAAGCTGAGAGACATCGCCAGGAAGCATGGCTGCGCCTTCTTGGTGGACGA GGTACAGACCGGAGGAGGCTGCACGGGCAAGTTCTGGGCCCATGAGCACTGGGGCCTGGATGACCCAGCA GACGTGATGACCTTCAGCAAGAAAATGATGACTGGGGGCTTCTTCCACAAGGAGGAGTTCAGGCCTAATG CTCCCTACCGGATCTTCAACACCTGGCTGGGGGACCCGTCCAAGAACCTGTTGCTGGCTGAGGTCATCAA CATCATCAAGCGGGAGGACCTGCTAAATAATGCAGCCCATGCCGGGAAGGCCCTGCTCACAGGACTGCTG GACCTCCAGGCCCGGTACCCCCAGTTCATCAGCAGGGTGAGAGGACGAGGCACCTTTTGCTCCTTCGATA CTCCCGATGATTCCATACGGAATAAGCTCATTTTAATTGCCAGAAACAAAGGTGTGGTGTTGGGTGGCTG TGGTGACAAATCCATTCGTTTCCGTCCCACGCTGGTCTTCAGGGATCACCACGCTCACCTGTTCCTCAAT ATTTTCAGTGACATCTTAGCAGACTTCAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC225860 protein sequence
Red=Cloning site Green=Tags(s) MASMLLAQRLACSFQHSYRLLVPGSRHISQAAAKVDVEFDYDGPLMKTEVPGPRSRELMKQLNIIQNAEA VHFFCNYEESRGNYLVDVDGNRMLDLYSQISSVPIGYSHPALLKLIQQPQNASMFVNRPALGILPPENFV EKLRQSLLSVAPKGMSQLITMACGSCSNENALKTIFMWYRSKERGQRGFSQEELETCMINQAPGCPDYSI LSFMGAFHGRTMGCLATTHSKAIHKIDIPSFDWPIAPFPRLKYPLEEFVKENQQEEARCLEEVEDLIVKY RKKKKTVAGIIVEPIQSEGGDNHASDDFFRKLRDIARKHGCAFLVDEVQTGGGCTGKFWAHEHWGLDDPA DVMTFSKKMMTGGFFHKEEFRPNAPYRIFNTWLGDPSKNLLLAEVINIIKREDLLNNAAHAGKALLTGLL DLQARYPQFISRVRGRGTFCSFDTPDDSIRNKLILIARNKGVVLGGCGDKSIRFRPTLVFRDHHAHLFLN IFSDILADFK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001127448 |
ORF Size | 1500 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001127448.1, NP_001120920.1 |
RefSeq Size | 4908 bp |
RefSeq ORF | 1503 bp |
Locus ID | 18 |
Cytogenetics | 16p13.2 |
Protein Families | Druggable Genome |
Protein Pathways | Alanine, aspartate and glutamate metabolism, beta-Alanine metabolism, Butanoate metabolism, Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation |
MW | 56.5 kDa |
Gene Summary | '4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC323108 | ABAT (untagged)-Human 4-aminobutyrate aminotransferase (ABAT), transcript variant 3 |
USD 760.00 |
|
RG225860 | ABAT (GFP-tagged) - Human 4-aminobutyrate aminotransferase (ABAT), transcript variant 3 |
USD 530.00 |
|
RC225860L3 | Lenti ORF clone of Human 4-aminobutyrate aminotransferase (ABAT), transcript variant 3, Myc-DDK-tagged |
USD 680.00 |
|
RC225860L4 | Lenti ORF clone of Human 4-aminobutyrate aminotransferase (ABAT), transcript variant 3, mGFP tagged |
USD 680.00 |
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