KLC2 (NM_001134775) Human Tagged ORF Clone

CAT#: RC226034

KLC2 (Myc-DDK-tagged)-Human kinesin light chain 2 (KLC2), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY427492) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226035 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified KLC2 protein (Cat# TP326034). The protein was produced from HEK293T cells transfected with KLC2 cDNA clone (Cat# RC226034) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KLC2
• Synonyms: SPOAN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226034 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGATGGTGTTTCCGCGGGAGGAGAAGCTGAGCCAGGATGAGATCGTGCTGGGCACCAAGGCTG
  TCATCCAGGGACTGGAGACTCTGCGTGGGGAGCATCGTGCCCTGCTGGCTCCTCTGGTTGCACCTGAGGC
  CGGCGAAGCCGAGCCTGGCTCGCAGGAGCGCTGCATCCTCCTGCGTCGCTCCCTGGAAGCCATTGAGCTT
  GGGCTGGGGGAGGCCCAGGTGATCTTGGCATTGTCGAGCCACCTGGGGGCTGTAGAATCAGAGAAGCAGA
  AGCTGCGGGCGCAGGTGCGGCGTCTGGTGCAGGAGAACCAGTGGCTGCGTGAGGAGCTGGCGGGGACACA
  GCAGAAGCTGCAGCGCAGTGAGCAGGCCGTGGCCCAGCTCGAGGAGGAGAAGCAGCACTTGCTGTTCATG
  AGCCAGATCCGCAAGTTGGATGAAGACGCCTCCCCTAACGAGGAGAAGGGGGACGTCCCCAAAGACACAC
  TGGATGACCTGTTCCCCAATGAGGATGAGCAGAGCCCAGCCCCTAGCCCAGGAGGAGGGGATGTGTCTGG
  TCAGCATGGGGGCTACGAGATCCCGGCCCGGCTCCGCACCCTGCACAACCTGGTGATCCAATACGCCTCA
  CAGGGCCGCTACGAGGTAGCTGTGCCACTCTGCAAGCAGGCACTCGAAGACCTGGAGAAGACGTCAGGCC
  ACGACCACCCTGACGTTGCCACCATGCTGAACATCCTGGCACTGGTCTATCGGGATCAGAACAAGTACAA
  GGAGGCTGCCCACCTGCTCAATGATGCTCTGGCCATCCGGGAGAAAACACTGGGCAAGGACCACCCAGCC
  GTGGCTGCGACACTAAACAACCTGGCAGTCCTGTATGGCAAGAGGGGCAAGTACAAGGAGGCTGAGCCAT
  TGTGCAAGCGGGCACTGGAGATCCGGGAGAAGGTCCTGGGCAAGTTTCACCCAGATGTGGCCAAGCAGCT
  CAGCAACCTGGCCCTGCTGTGCCAGAACCAGGGCAAAGCTGAGGAGGTGGAATATTACTATCGGCGGGCA
  CTGGAGATCTATGCTACACGCCTCGGGCCCGATGACCCCAATGTGGCCAAGACCAAGAACAACCTGGCTT
  CCTGCTACCTGAAGCAGGGCAAGTACCAGGATGCGGAGACCTTGTACAAGGAGATCCTCACCCGCGCTCA
  TGAGAAAGAGTTTGGCTCTGTCAATGGGGACAACAAGCCCATCTGGATGCACGCAGAGGAGCGGGAGGAA
  AGCAAGGATAAGCGCCGGGACAGCGCCCCCTATGGGGAATACGGCAGCTGGTACAAGGCCTGTAAAGTAG
  ACAGCCCCACAGTCAACACCACCCTGCGCAGCTTGGGGGCCCTATACCGGCGCCAGGGCAAGCTGGAAGC
  CGCGCACACACTAGAGGACTGTGCCAGCCGTAACCGCAAGCAGGGTTTGGACCCCGCAAGCCAGACCAAG
  GTGGTAGAACTGCTGAAAGATGGCAGTGGCAGGCGGGGAGACCGCCGCAGCAGCCGAGACATGGCTGGGG
  GTGCCGGGCCTCGGTCTGAGTCTGACCTCGAGGACGTGGGACCTACAGCTGAGTGGAATGGGGATGGCAG
  TGGCTCCTTGAGGCGCAGCGGTTCCTTTGGGAAACTCCGGGATGCCCTGAGGCGCAGCAGTGAGATGCTG
  GTAAAGAAGCTGCAGGGGGGCACCCCCCAGGAGCCCCCTAACCCCAGGATGAAGCGGGCCAGTTCCCTCA
  ACTTCCTCAACAAGAGCGTGGAAGAGCCGACCCAGCCTGGAGGCACAGGTCTCTCTGACAGCCGCACTCT
  CAGCTCCAGCTCCATGGACCTCTCCCGACGAAGCTCCCTGGTGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226034 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAMMVFPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLVAPEAGEAEPGSQERCILLRRSLEAIEL
  GLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKLQRSEQAVAQLEEEKQHLLFM
  SQIRKLDEDASPNEEKGDVPKDTLDDLFPNEDEQSPAPSPGGGDVSGQHGGYEIPARLRTLHNLVIQYAS
  QGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKEAAHLLNDALAIREKTLGKDHPA
  VAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRA
  LEIYATRLGPDDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGDNKPIWMHAEEREE
  SKDKRRDSAPYGEYGSWYKACKVDSPTVNTTLRSLGALYRRQGKLEAAHTLEDCASRNRKQGLDPASQTK
  VVELLKDGSGRRGDRRSSRDMAGGAGPRSESDLEDVGPTAEWNGDGSGSLRRSGSFGKLRDALRRSSEML
  VKKLQGGTPQEPPNPRMKRASSLNFLNKSVEEPTQPGGTGLSDSRTLSSSSMDLSRRSSLVG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001134775
• ORF Size: 1866 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001134775.1, NP_001128247.1
• RefSeq Size: 3093 bp
• RefSeq ORF: 1869 bp
• Locus ID: 64837
• Protein Families: Druggable Genome
• MW: 68.9 kDa
• Gene Summary: The protein encoded by this gene is a light chain of kinesin, a molecular motor responsible for moving vesicles and organelles along microtubules. Defects in this gene are a cause of spastic paraplegia, optic atrophy, and neuropathy (SPOAN) syndrome. [provided by RefSeq, Mar 2016]