ZNF195 (NM_001130520) Human Tagged ORF Clone
CAT#: RC226042
- TrueORF®
ZNF195 (Myc-DDK-tagged)-Human zinc finger protein 195 (ZNF195), transcript variant 1. Note: ORF is codon optimized
"NM_001130520" in other vectors (2)
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ZNF195 |
Synonyms | HRF1; ZNFP104 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC226042 ORF sequence, codon optimized.
Due to the complexity of NM_001130520, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTTTGCTGACCTTTCGGGATGTCGCCATTGAATTCAGTCTGGAAGAATGGAAGTGCCTGGATCTGG CTCAGCAAAACTTGTACAGGGATGTAATGCTGGAAAATTACCGCAACCTGTTCAGTGTCGGGCTGACCGT GTGTAAGCCAGGGCTCATTACATGTCTTGAACAGCGGAAGGAGCCCTGGAATGTGAAACGCCAAGAGGCT GCCGACGGGCATCCAGAGATGGGCTTCCATCACGCAACCCAGGCTTGTCTCGAACTCCTGGGCTCATCTG ACCTGCCCGCTAGCGCGTCTCAGTCTGCTGGAATCACGGGGGTGAACCATCGGGCTCAGCCTGGCCTGAA CGTTAGTGTAGACAAATTCACCGCCTTGTGTAGCCCTGGGGTCCTGCAAACTGTGAAGTGGTTTCTGGAG TTTCGCTGTATTTTTAGTCTGGCCATGAGCTCACACTTCACTCAGGACCTCTTGCCTGAGCAGGGAATTC AGGACGCATTTCCTAAGCGGATCCTCCGGGGTTACGGGAATTGTGGGCTGGACAACCTTTACCTGCGCAA GGATTGGGAATCTTTGGACGAATGTAAGCTGCAGAAGGATTACAACGGCCTGAACCAGTGCTCTTCTACC ACTCATAGTAAGATCTTCCAGTATAACAAGTACGTAAAAATCTTCGATAACTTTTCTAACTTGCACCGCA GAAATATATCCAACACCGGTGAGAAGCCCTTTAAATGTCAGGAATGCGGGAAGTCATTTCAGATGCTCAG TTTTCTCACAGAACACCAAAAAATCCACACCGGCAAGAAGTTTCAGAAGTGCGGTGAGTGCGGGAAGACC TTTATCCAGTGCTCCCATTTTACTGAGCCTGAAAATATTGACACAGGTGAGAAGCCATATAAATGTCAGG AGTGCAATAATGTCATCAAGACCTGTTCTGTGCTGACCAAGAACAGGATTTACGCAGGAGGAGAACACTA CCGCTGTGAGGAGTTCGGGAAAGTGTTTAACCAGTGCAGCCACCTTACGGAACACGAGCATGGCACTGAG GAAAAGCCCTGCAAGTATGAAGAGTGCAGCAGTGTTTTCATCTCCTGTTCATCCCTGAGTAACCAGCAGA TGATCCTGGCCGGCGAGAAATTGAGCAAATGCGAAACCTGGTACAAAGGATTCAACCACTCACCTAATCC TTCTAAACATCAAAGAAATGAGATAGGAGGAAAACCTTTCAAGTGTGAGGAATGTGATTCCATTTTTAAA TGGTTTAGTGACCTGACTAAGCACAAGCGGATTCACACTGGCGAGAAGCCATACAAGTGTGATGAGTGCG GGAAGGCCTACACTCAAAGTTCTCATCTCTCAGAACATCGCCGCATTCACACCGGCGAGAAGCCCTACCA GTGTGAGGAATGCGGCAAGGTATTCAGGACCTGTAGCAGCCTCTCAAATCACAAGAGGACCCACAGTGAG GAGAAGCCATATACCTGTGAGGAGTGCGGAAACATATTTAAGCAGCTTAGCGACCTGACCAAACACAAGA AGACCCATACAGGAGAGAAACCTTACAAGTGCGACGAATGCGGAAAAAACTTCACACAGTCATCCAATCT GATCGTTCACAAGAGAATACACACTGGCGAGAAGCCCTACAAGTGCGAAGAGTGTGGTAGGGTATTCATG TGGTTCTCCGACATAACAAAGCACAAGAAGACTCATACTGGAGAGAAACCCTATAAGTGTGACGAGTGCG GTAAAAACTTTACGCAGAGTTCCAATCTGATCGTGCATAAGCGCATACATACCGGAGAGAAACCATACAA GTGCGAGAAATGCGGAAAAGCCTTTACCCAGTTTAGCCACCTGACAGTGCATGAGAGCATCCATACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC226042 representing NM_001130520
Red=Cloning site Green=Tags(s) MTLLTFRDVAIEFSLEEWKCLDLAQQNLYRDVMLENYRNLFSVGLTVCKPGLITCLEQRKEPWNVKRQEA ADGHPEMGFHHATQACLELLGSSDLPASASQSAGITGVNHRAQPGLNVSVDKFTALCSPGVLQTVKWFLE FRCIFSLAMSSHFTQDLLPEQGIQDAFPKRILRGYGNCGLDNLYLRKDWESLDECKLQKDYNGLNQCSST THSKIFQYNKYVKIFDNFSNLHRRNISNTGEKPFKCQECGKSFQMLSFLTEHQKIHTGKKFQKCGECGKT FIQCSHFTEPENIDTGEKPYKCQECNNVIKTCSVLTKNRIYAGGEHYRCEEFGKVFNQCSHLTEHEHGTE EKPCKYEECSSVFISCSSLSNQQMILAGEKLSKCETWYKGFNHSPNPSKHQRNEIGGKPFKCEECDSIFK WFSDLTKHKRIHTGEKPYKCDECGKAYTQSSHLSEHRRIHTGEKPYQCEECGKVFRTCSSLSNHKRTHSE EKPYTCEECGNIFKQLSDLTKHKKTHTGEKPYKCDECGKNFTQSSNLIVHKRIHTGEKPYKCEECGRVFM WFSDITKHKKTHTGEKPYKCDECGKNFTQSSNLIVHKRIHTGEKPYKCEKCGKAFTQFSHLTVHESIHT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001130520 |
ORF Size | 1887 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001130520.1, NM_001130520.2, NP_001123992.1 |
RefSeq Size | 3187 bp |
RefSeq ORF | 1890 bp |
Locus ID | 7748 |
Cytogenetics | 11p15.4 |
Protein Families | Transcription Factors |
MW | 72.3 kDa |
Gene Summary | 'This gene encodes a protein belonging to the Krueppel C2H2-type zinc-finger protein family. These family members are transcription factors that are implicated in a variety of cellular processes. This gene is located near the centromeric border of chromosome 11p15.5, next to an imprinted domain that is associated with maternal-specific loss of heterozygosity in Wilms' tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]' |
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