PAK6 (NM_001128628) Human Tagged ORF Clone

CAT#: RC226088

PAK6 (Myc-DDK-tagged)-Human p21 protein (Cdc42/Rac)-activated kinase 6 (PAK6), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY426985) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226088 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BUB1B-PAK6
• Synonyms: PAK-5; PAK-6; PAK5; PAK6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226088 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCGCAAGAAAAAGAAGAAACGCCCTGAGATCTCAGCGCCACAGAACTTCCAGCACCGTGTCCACA
  CCTCCTTCGACCCCAAAGAAGGCAAGTTTGTGGGCCTCCCCCCACAATGGCAGAACATCCTGGACACACT
  GCGGCGCCCCAAGCCCGTGGTGGACCCTTCGCGAATCACACGGGTGCAGCTCCAGCCCATGAAGACAGTG
  GTGCGGGGCAGCGCGATGCCTGTGGATGGCTACATCTCGGGGCTGCTCAACGACATCCAGAAGTTGTCAG
  TCATCAGCTCCAACACCCTGCGTGGCCGCAGCCCCACCAGCCGGCGGCGGGCACAGTCCCTGGGGCTGCT
  GGGGGATGAGCACTGGGCCACCGACCCAGACATGTACCTCCAGAGCCCCCAGTCTGAGCGCACTGACCCC
  CACGGCCTCTACCTCAGCTGCAACGGGGGCACACCAGCAGGCCACAAGCAGATGCCGTGGCCCGAGCCAC
  AGAGCCCACGGGTCCTGCCCAATGGGCTGGCTGCAAAGGCACAGTCCCTGGGCCCCGCCGAGTTTCAGGG
  TGCCTCGCAGCGCTGTCTGCAGCTGGGTGCCTGCCTGCAGAGCTCCCCACCAGGAGCCTCGCCCCCCACG
  GGCACCAATAGGCATGGAATGAAGGCTGCCAAGCATGGCTCTGAGGAGGCCCGGCCACAGTCCTGCCTGG
  TGGGCTCAGCCACAGGCAGGCCAGGTGGGGAAGGCAGCCCTAGCCCTAAGACCCGGGAGAGCAGCCTGAA
  GCGCAGGCTATTCCGAAGCATGTTCCTGTCCACTGCTGCCACAGCCCCTCCAAGCAGCAGCAAGCCAGGC
  CCTCCACCACAGAGCAAGCCCAACTCCTCTTTCCGACCGCCGCAGAAAGACAACCCCCCAAGCCTGGTGG
  CCAAGGCCCAGTCCTTGCCCTCGGACCAGCCGGTGGGGACCTTCAGCCCTCTGACCACTTCGGATACCAG
  CAGCCCCCAGAAGTCCCTCCGCACAGCCCCGGCCACAGGCCAGCTTCCAGGCCGGTCTTCCCCAGCGGGA
  TCCCCCCGCACCTGGCACGCCCAGATCAGCACCAGCAACCTGTACCTGCCCCAGGACCCCACGGTTGCCA
  AGGGTGCCCTGGCTGGTGAGGACACAGGTGTTGTGACACATGAGCAGTTCAAGGCTGCGCTCAGGATGGT
  GGTGGACCAGGGTGACCCCCGGCTGCTGCTGGACAGCTACGTGAAGATTGGCGAGGGCTCCACCGGCATC
  GTCTGCTTGGCCCGGGAGAAGCACTCGGGCCGCCAGGTGGCCGTCAAGATGATGGACCTCAGGAAGCAGC
  AGCGCAGGGAGCTGCTCTTCAACGAGGTGGTGATCATGCGGGACTACCAGCACTTCAACGTGGTGGAGAT
  GTACAAGAGCTACCTGGTGGGCGAGGAGCTGTGGGTGCTCATGGAGTTCCTGCAGGGAGGAGCCCTCACA
  GACATCGTCTCCCAAGTCAGGCTGAATGAGGAGCAGATTGCCACTGTGTGTGAGGCTGTGCTGCAGGCCC
  TGGCCTACCTGCATGCTCAGGGTGTCATCCACCGGGACATCAAGAGTGACTCCATCCTGCTGACCCTCGA
  TGGCAGGGTGAAGCTCTCGGACTTCGGATTCTGTGCTCAGATCAGCAAAGACGTCCCTAAGAGGAAGTCC
  CTGGTGGGAACCCCCTACTGGATGGCTCCTGAAGTGATCTCCAGGTCTTTGTATGCCACTGAGGTGGATA
  TCTGGTCTCTGGGCATCATGGTGATTGAGATGGTAGATGGGGAGCCACCGTACTTCAGTGACTCCCCAGT
  GCAAGCCATGAAGAGGCTCCGGGACAGCCCCCCACCCAAGCTGAAAAACTCTCACAAGGTCTCCCCAGTG
  CTGCGAGACTTCCTGGAGCGGATGCTGGTGCGGGACCCCCAAGAGAGAGCCACAGCCCAGGAGCTCCTAG
  ACCACCCCTTCCTGCTGCAGACAGGGCTACCTGAGTGCCTGGTGCCCCTGATCCAGCTCTACCGAAAGCA
  GACCTCCACCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226088 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MFRKKKKKRPEISAPQNFQHRVHTSFDPKEGKFVGLPPQWQNILDTLRRPKPVVDPSRITRVQLQPMKTV
  VRGSAMPVDGYISGLLNDIQKLSVISSNTLRGRSPTSRRRAQSLGLLGDEHWATDPDMYLQSPQSERTDP
  HGLYLSCNGGTPAGHKQMPWPEPQSPRVLPNGLAAKAQSLGPAEFQGASQRCLQLGACLQSSPPGASPPT
  GTNRHGMKAAKHGSEEARPQSCLVGSATGRPGGEGSPSPKTRESSLKRRLFRSMFLSTAATAPPSSSKPG
  PPPQSKPNSSFRPPQKDNPPSLVAKAQSLPSDQPVGTFSPLTTSDTSSPQKSLRTAPATGQLPGRSSPAG
  SPRTWHAQISTSNLYLPQDPTVAKGALAGEDTGVVTHEQFKAALRMVVDQGDPRLLLDSYVKIGEGSTGI
  VCLAREKHSGRQVAVKMMDLRKQQRRELLFNEVVIMRDYQHFNVVEMYKSYLVGEELWVLMEFLQGGALT
  DIVSQVRLNEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRKS
  LVGTPYWMAPEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSPPPKLKNSHKVSPV
  LRDFLERMLVRDPQERATAQELLDHPFLLQTGLPECLVPLIQLYRKQTSTC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001128628
• ORF Size: 2043 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001128628.1, NM_001128628.2, NP_001122100.1
• RefSeq Size: 3891 bp
• RefSeq ORF: 2046 bp
• Locus ID: 106821730
• MW: 74.9 kDa
• Gene Summary: This gene represents readthrough transcription between the genes BUB1B (mitotic checkpoint serine/threonine-protein kinase BUB1 beta) and PAK6 (serine/threonine-protein kinase PAK 6). The protein encoded by the readthrough transcripts is the same as the product of the downstream gene (PAK6). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]