alpha Actinin (ACTN1) (NM_001130005) Human Tagged ORF Clone
CAT#: RC226236L3
- LentiORF®
Lenti-ORF clone of ACTN1 (Myc-DDK-tagged)-Human actinin, alpha 1 (ACTN1), transcript variant 3
"NM_001130005" in other vectors (4)
Product Images
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Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | ACTN1 |
Synonyms | BDPLT15 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC226236).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_001130005 |
ORF Size | 2661 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001130005.1, NP_001123477.1 |
RefSeq ORF | 2664 bp |
Locus ID | 87 |
Cytogenetics | 14q22-q24 |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS |
Protein Pathways | Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction |
MW | 102.5 kDa |
Gene Summary | 'Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC323227 | ACTN1 (untagged)-Human actinin, alpha 1 (ACTN1), transcript variant 3 |
USD 1,800.00 |
|
RC226236 | ACTN1 (Myc-DDK-tagged)-Human actinin, alpha 1 (ACTN1), transcript variant 3 |
USD 990.00 |
|
RG226236 | ACTN1 (GFP-tagged) - Human actinin, alpha 1 (ACTN1), transcript variant 3 |
USD 1,090.00 |
|
RC226236L4 | Lenti-ORF clone of ACTN1 (mGFP-tagged)-Human actinin, alpha 1 (ACTN1), transcript variant 3 |
USD 1,190.00 |
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