ARAP1 (NM_001135190) Human Tagged ORF Clone

CAT#: RC226338

ARAP1 (Myc-DDK-tagged)-Human ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant 4

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARAP1
• Synonyms: CENTD2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226338 representing NM_001135190
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAAGAAGGAGGAGCCCCCACCGAGCCGAGTCCCACGGGCCGTGCGCGTGGCCAGTCTGCTGAGCG
  AGGGAGAGGAACTGTCTGGGGACGACCAAGGGGATGAGGAAGAGGATGACCACGCCTATGAGGGCGTCCC
  CAATGGCGGATGGCATACCAGCAGCCTGAGCTTGTCCTTGCCCAGCACAATAGCTGCGCCACACCCCATG
  GACGGGCCGCCTGGGGGCTCCACCCCCGTCACACCAGTCATCAAGGCTGGCTGGCTGGACAAGAACCCAC
  CGCAGGGATCTTACATCTATCAGAAACGATGGGTGAGACTGGATACTGATCACCTGCGATACTTTGACAG
  TAACAAGGACGCTTACTCTAAGCGCTTTATCTCTGTGGCCTGCATCTCCCACGTGGCTGCCATCGGGGAC
  CAGAAGTTTGAAGTGATCACAAACAACCGAACCTTTGCCTTCCGGGCAGAGAGTGATGTGGAGCGGAAGG
  AGTGGATGCAGGCCCTGCAGCAGGCCATGGCTGAGCAGCGTGCCCGGGCCCGGCTCTCTAGCGCTTATCT
  GCTGGGAGTTCCAGGCTCAGAGCAGCCTGACCGCGCTGGCAGCCTGGAGCTTCGTGGCTTCAAGAATAAG
  CTGTACGTGGCCGTGGTCGGGGACAAAGTGCAGCTCTACAAGAATCTAGAGGAGTACCACCTGGGCATTG
  GCATCACCTTCATCGACATGAGCGTGGGCAACGTGAAGGAAGTGGACCGGCGCAGCTTCGACCTCACCAC
  GCCCTACCGCATCTTCAGCTTCTCTGCTGACTCAGAGCTAGAGAAGGAGCAGTGGTTGGAGGCCATGCAG
  GGAGCCATCGCTGAGGCCCTGTCTACCTCGGAGGTGGCCGAGCGCATCTGGGCTGCAGCCCCCAACAGGT
  TCTGTGCTGACTGCGGGGCTCCTCAGCCTGACTGGGCCTCCATCAACCTCTGTGTTGTTATCTGCAAGCG
  CTGTGCAGGGGAGCACCGTGGCCTGGGCGCTGGCGTCTCCAAGGTGCGGAGCCTGAAGATGGACAGGAAG
  GTGTGGACAGAAACACTTATCGAGGCCCTGTGTGCTGCAGTCACCACCACAGACCTGGCTGAGACCCAGG
  CGCTCCTGGGCTGTGGGGCTGGGATCAACTGCTTCTCGGGGGACCCTGAGGCCCCCACGCCCCTGGCTCT
  TGCAGAGCAGGCGGGGCAGACGCTGCAGATGGAATTCCTTCGGAACAACCGGACCACAGAGGTGCCTCGG
  CTGGACTCGATGAAGCCCCTGGAAAAGCACTACTCAGTTGTCCTGCCGACCGTGAGCCACAGTGGCTTCC
  TCTACAAGACTGCCTCTGCCGGCAAGCTGCTACAGGACCGCCGGGCCCGGGAAGAGTTCAGCCGGCGCTG
  GTGTGTCCTTGGTGACGGGGTCCTGAGCTACTTTGAGAATGAGCGGGCAGTGACCCCCAATGGAGAGATT
  CGGGCCAGCGAGATTGTGTGCCTGGCAGTGCCCCCTCCTGACACCCATGGCTTTGAGCACACCTTTGAGG
  TGTACACGGAGGGAGAACGGCTGTACCTGTTTGGGCTGGAGAGTGCGGAGCAGGCTCATGAGTGGGTCAA
  GTGTATTGCTAAGGCATTCGTGCCTCCCCTAGCCGAGGATCTGCTGGCCCGGGATTTTGAGCGGCTGGGA
  CGCCTACCCTACAAAGCTGGCCTGAGCCTACAGCGGGCCCAGGAGGGCTGGTTCTCTCTCAGTGGCTCGG
  AGCTCCGTGCTGTCTTCCCGGAGGGGCCCTGCGAAGAGCCGCTGCAACTACGGAAACTGCAGGAGCTTTC
  CATCCAGGGGGACAGTGAGAACCAGGTGCTGGTGCTGGTGGAGCGAAGGAGGACACTGTACATACAGGGC
  GAGCGGCGGCTGGACTTCATGGGTTGGCTGGGGGCCATCCAGAAAGCAGCCGCCAGCATGGGGGACACGC
  TGTCGGAGCAGCAGCTTGGGGACTCGGATATCCCGGTGATCGTGTACCGCTGTGTGGACTACATCACGCA
  GTGCGGCCTGACCTCCGAGGGCATCTACCGCAAGTGTGGGCAGACATCGAAGACACAGCGGCTGCTGGAG
  AGCCTGCGGCAGGATGCGCGCTCTGTGCACCTCAAGGAGGGCGAGCAGCACGTGGATGATGTTTCCTCGG
  CGCTCAAGCGCTTCCTGCGCGACCTGCCTGATGGGCTCTTCACTCGCGCCCAGCGCCTAACCTGGCTGGA
  GGCCTCAGAGATTGAGGACGAGGAGGAGAAGGTCTCCAGGTACCGAGAGCTGCTGGTGCGGCTGCCCCCT
  GTCAACCGGGCCACAGTGAAGGCCCTTATCAGCCACCTGTACTGTGTTCAGTGCTTCTCAGACACGAACC
  AGATGAACGTGCACAACCTGGCAATTGTGTTTGGGCCCACGCTCTTCCAGACAGATGGGCAGGACTACAA
  GGCTGGCCGTGTGGTGGAAGACCTCATTAACCACTATGTGGTGGTGTTTAGTGTGGATGAGGAAGAGCTC
  AGGAAGCAGCGGGAGGAGATCACTGCCATTGTGAAGATGCGCGTGGCTGGCACTGCCAGTGGGACCCAGC
  ATGCCGGTGACTTCATCTGCACAGTGTATCTGGAAGAGAAGAAGGCAGAGACTGAGCAGCATATCAAGGT
  CCCAGCATCCATGACTGCTGAGGAGCTCACCCTGGAGATCCTGGATCGCCGGAACGTGGGCATCAGGGAG
  AAGGACTATTGGACCTGCTTTGAGGTCAACGAGAGGGAGGAGGCAGAGCGCCCCCTGCACTTTGCGGAGA
  AGGTGCTGCCCATCCTGCACGGGCTGGGCACGGACAGCCACCTGGTGGTGAAGAAGCACCAGGCCATGGA
  GGCCATGCTGCTGTACCTGGCCAGCCGTGTCGGTGACACCAAGCATGGCATGATGAAGTTCCGTGAGGAC
  CGCAGCCTCCTGGGCCTGGGCCTGCCCTCAGGTGGCTTCCACGATCGCTACTTCATCCTCAACAGCAGCT
  GCTTGCGGCTCTACAAGGAGGTCCGGAGTCACCGGCCTGAGAAGGAGTGGCCTATTAAGAGTCTCAAAGT
  CTACCTGGGAGTGAAGAAGAAACTCAGGCCACCCACCTGCTGGGGCTTCACAGTGGTGCATGAGACAGAG
  AAACATGAGAAGCAGCAGTGGTACCTCTGCTGTGACACACAGATGGAGCTCCGGGAGTGGTTCGCTACCT
  TTCTGTTTGTGCAGCATGACGGCCTGGTGTGGCCCTCAGAGCCCTCACGCGTGTCCCGGGCAGTGCCTGA
  GGTCCGGCTGGGTAGTGTGTCACTGATCCCCCTTCGAGGTAGTGAAAATGAAATGCGCCGGAGTGTGGCT
  GCCTTCACCGCGGACCCTCTGTCTCTTCTGCGCAACGTC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226338 representing NM_001135190
  Red=Cloning site Green=Tags(s)
  
  MTKKEEPPPSRVPRAVRVASLLSEGEELSGDDQGDEEEDDHAYEGVPNGGWHTSSLSLSLPSTIAAPHPM
  DGPPGGSTPVTPVIKAGWLDKNPPQGSYIYQKRWVRLDTDHLRYFDSNKDAYSKRFISVACISHVAAIGD
  QKFEVITNNRTFAFRAESDVERKEWMQALQQAMAEQRARARLSSAYLLGVPGSEQPDRAGSLELRGFKNK
  LYVAVVGDKVQLYKNLEEYHLGIGITFIDMSVGNVKEVDRRSFDLTTPYRIFSFSADSELEKEQWLEAMQ
  GAIAEALSTSEVAERIWAAAPNRFCADCGAPQPDWASINLCVVICKRCAGEHRGLGAGVSKVRSLKMDRK
  VWTETLIEALCAAVTTTDLAETQALLGCGAGINCFSGDPEAPTPLALAEQAGQTLQMEFLRNNRTTEVPR
  LDSMKPLEKHYSVVLPTVSHSGFLYKTASAGKLLQDRRAREEFSRRWCVLGDGVLSYFENERAVTPNGEI
  RASEIVCLAVPPPDTHGFEHTFEVYTEGERLYLFGLESAEQAHEWVKCIAKAFVPPLAEDLLARDFERLG
  RLPYKAGLSLQRAQEGWFSLSGSELRAVFPEGPCEEPLQLRKLQELSIQGDSENQVLVLVERRRTLYIQG
  ERRLDFMGWLGAIQKAAASMGDTLSEQQLGDSDIPVIVYRCVDYITQCGLTSEGIYRKCGQTSKTQRLLE
  SLRQDARSVHLKEGEQHVDDVSSALKRFLRDLPDGLFTRAQRLTWLEASEIEDEEEKVSRYRELLVRLPP
  VNRATVKALISHLYCVQCFSDTNQMNVHNLAIVFGPTLFQTDGQDYKAGRVVEDLINHYVVVFSVDEEEL
  RKQREEITAIVKMRVAGTASGTQHAGDFICTVYLEEKKAETEQHIKVPASMTAEELTLEILDRRNVGIRE
  KDYWTCFEVNEREEAERPLHFAEKVLPILHGLGTDSHLVVKKHQAMEAMLLYLASRVGDTKHGMMKFRED
  RSLLGLGLPSGGFHDRYFILNSSCLRLYKEVRSHRPEKEWPIKSLKVYLGVKKKLRPPTCWGFTVVHETE
  KHEKQQWYLCCDTQMELREWFATFLFVQHDGLVWPSEPSRVSRAVPEVRLGSVSLIPLRGSENEMRRSVA
  AFTADPLSLLRNV
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135190
• ORF Size: 3399 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001135190.1, NP_001128662.1
• RefSeq ORF: 3402
• Locus ID: 116985
• Protein Pathways: Endocytosis
• MW: 127.5 kDa
• Gene Summary: The protein encoded by this gene contains SAM, ARF-GAP, RHO-GAP, ankyrin repeat, RAS-associating, and pleckstrin homology (PH) domains. In vitro, this protein displays RHO-GAP and phosphatidylinositol (3,4,5) trisphosphate (PIP3)-dependent ARF-GAP activity. The encoded protein associates with the Golgi, and the ARF-GAP activity mediates changes in the Golgi and the formation of filopodia. It is thought to regulate the cell-specific trafficking of a receptor protein involved in apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]