Centaurin gamma 1 (AGAP2) (NM_001122772) Human Tagged ORF Clone

CAT#: RC226356

AGAP2 (Myc-DDK-tagged)-Human ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 (AGAP2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY426561) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226356 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AGAP2
• Synonyms: CENTG1; GGAP2; PIKE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226356 representing NM_001122772
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCGGGGCGCGGGCGCGCTTCAGCGCCGGACAACGACCTACCTCATCTCGCTGACCCTGGTTAAGC
  TCGAGTCGGTGCCTCCGCCGCCGCCTTCTCCGTCTGCGGCCGCGGCCGGCGCCGCCGGTGCCAGAGGCTC
  CGAGACTGGGGATCCTGGCAGCCCCCGAGGCGCGGAGGAGCCGGGCAAGAAGCGGCACGAACGTCTCTTC
  CACCGGCAGGATGCGCTGTGGATCAGCACGAGCAGCGCGGGCACCGGGGGCGCGGAGCCCCCAGCCCTGT
  CCCCGGCTCCGGCCAGTCCGGCCCGCCCAGTCTCCCCCGCTCCCGGCCGCCGCCTCTCCCTCTGGGCCGT
  CCCTCCGGGACCCCCGCTCTCCGGGGGACTGAGCCCCGACCCCAAGCCTGGGGGCGCCCCCACCTCCTCC
  CGGCGCCCCCTGCTCAGCAGCCCGAGCTGGGGCGGCCCGGAGCCCGAAGGCCGGGCGGGCGGCGGCATCC
  CTGGCTCATCCTCTCCGCACCCTGGCACCGGCAGCCGGAGGCTCAAGGTGGCGCCTCCTCCGCCGGCTCC
  CAAGCCTTGCAAGACCGTGACCACGAGTGGAGCCAAAGCCGGCGGGGGCAAGGGCGCGGGTAGCCGCCTG
  TCATGGCCCGAAAGCGAGGGCAAGCCCAGGGTCAAGGGGTCAAAGAGCAGCGCCGGGACTGGAGCTTCGG
  TCTCTGCCGCCGCCACCGCCGCCGCCGCCGGGGGAGGGGGCTCTACAGCTTCGACCTCTGGTGGGGTCGG
  GGCTGGGGCTGGAGCCCGAGGGAAGTTGTCCCCTCGGAAAGGCAAGAGTAAGACCTTGGACAACAGTGAC
  TTGCATCCGGGACCGCCTGCCGGCTCTCCTCCTCCGCTAACCCTCCCACCAACTCCGAGTCCAGCCACTG
  CTGTCACCGCTGCTTCCGCGCAGCCCCCCGGGCCTGCACCTCCAATCACTCTGGAGCCTCCAGCTCCGGG
  GCTGAAACGGGGCCGGGAGGGGGGCCGAGCATCCACTCGTGACCGCAAGATGCTCAAGTTTATCAGCGGC
  ATCTTCACCAAGAGCACAGGAGGGCCTCCTGGCTCCGGGCCCCTTCCCGGACCCCCCAGCCTGTCTTCTG
  GCAGCGGGTCCAGGGAGCTGCTGGGCGCCGAGCTCCGCGCTTCCCCTAAGGCTGTGATCAATAGCCAGGA
  ATGGACTTTGAGCCGCTCCATTCCTGAACTGCGCCTGGGTGTGCTGGGCGATGCCAGGAGTGGGAAGTCA
  TCGCTCATCCACCGATTCCTGACTGGCTCATACCAGGTGCTGGAGAAGACAGAGAGTGAGCAGTACAAGA
  AAGAAATGTTGGTGGATGGACAGACACATCTGGTGCTAATCCGAGAGGAAGCTGGGGCACCTGATGCCAA
  GTTCTCAGGCTGGGCAGATGCTGTGATCTTCGTCTTCAGCCTGGAGGATGAGAACAGTTTCCAGGCTGTG
  AGCCGTCTCCATGGGCAGCTGAGTTCCCTTCGCGGGGAGGGACGAGGAGGCCTGGCCTTGGCACTGGTGG
  GGACACAAGACAGGATCAGTGCTTCCTCCCCTCGGGTGGTGGGAGATGCTCGTGCCAGAGCTCTGTGCGC
  GGACATGAAACGCTGCAGCTACTATGAGACTTGTGCAACCTATGGGCTCAATGTGGATCGGGTCTTCCAG
  GAGGTGGCCCAGAAGGTGGTGACCTTGCGCAAGCAGCAACAGCTTCTGGCTGCCTGCAAGTCCCTGCCCA
  GCTCCCCAAGCCACTCAGCTGCATCCACTCCGGTAGCTGGCCAGGCTAGTAACGGGGGCCACACTAGCGA
  CTACTCTTCTTCCCTCCCGTCCTCACCGAATGTTGGTCACCGGGAGCTCCGAGCCGAGGCAGCTGCAGTG
  GCTGGATTGAGCACCCCAGGGTCCCTGCACCGGGCAGCCAAGCGCAGGACCAGCCTTTTTGCGAATCGTC
  GGGGTAGTGACTCCGAGAAACGAAGCTTGGATAGTCGGGGAGAGACAACAGGGAGTGGGCGAGCCATCCC
  CATCAAACAGAGCTTCCTACTAAAACGAAGTGGCAATTCCTTGAACAAAGAATGGAAGAAGAAATATGTA
  ACCCTGTCCAGTAATGGCTTTCTACTCTACCACCCCAGTATTAACGATTACATCCACAGTACCCACGGCA
  AGGAGATGGACTTGCTGCGAACAACAGTCAAAGTCCCGGGCAAGCGGCCCCCGAGGGCCATCTCTGCCTT
  TGGCCCCTCAGCCAGCATTAACGGGCTCGTCAAGGACATGAGCACTGTCCAGATGGGTGAAGGCCTGGAA
  GCCACTACTCCCATGCCAAGCCCTAGCCCCAGCCCCAGTTCCCTGCAGCCACCACCAGATCAGACATCCA
  AACACCTGCTGAAGCCAGACCGGAATTTGGCCCGAGCCCTCAGCACGGACTGTACCCCATCTGGAGACCT
  GAGCCCCCTGAGTCGGGAACCCCCTCCTTCTCCCATGGTGAAGAAGCAGAGGAGGAAAAAATTGACAACA
  CCATCCAAGACTGAAGGCTCGGCTGGGCAGGCTGAAGCCAAGCGCAAAATGTGGAAACTAAAATCCTTTG
  GTAGTTTAAGAAATATTTATAAAGCAGAGGAAAACTTTGAGTTCCTGATCGTGTCCAGCACGGGTCAGAC
  GTGGCACTTTGAGGCAGCCAGTTTTGAGGAGCGGGATGCCTGGGTCCAGGCCATCGAGAGTCAGATCCTA
  GCCAGTCTGCAATGCTGTGAGAGCAGCAAGGTCAAGCTGCGCACAGACAGCCAAAGCGAGGCCGTGGCCA
  TCCAGGCGATCCGGAACGCCAAGGGGAATTCAATCTGCGTGGACTGCGGGGCCCCCAACCCCACGTGGGC
  CAGCTTGAACCTGGGCGCCCTCATCTGCATCGAGTGTTCTGGCATCCACCGCAACCTGGGCACACACCTG
  TCCCGCGTTCGCTCGCTGGACTTGGACGACTGGCCACGGGAGCTGACCCTGGTGCTGACGGCTATTGGCA
  ACGACACGGCCAACCGCGTGTGGGAAAGCGACACGCGAGGCCGTGCCAAGCCCTCGCGGGACTCTTCGCG
  GGAGGAGCGCGAGTCGTGGATTCGCGCCAAGTACGAGCAGCTACTGTTCCTGGCGCCGCTGAGCACCTCG
  GAGGAGCCGCTGGGCCGCCAGCTGTGGGCCGCCGTGCAGGCCCAGGACGTGGCTACCGTTCTCCTGCTTT
  TGGCCCATGCGCGACACGGGCCGCTCGACACCAGCGTAGAGGACCCACAGCTGCGCTCCCCACTCCACCT
  GGCGGCCGAGCTCGCCCACGTCGTCATCACGCAACTGCTGCTGTGGTACGGCGCGGACGTGGCGGCCCGT
  GACGCCCAGGGCCGCACGGCGCTGTTCTACGCCCGCCAGGCTGGAAGCCAGCTGTGCGCCGACATCCTTC
  TCCAGCACGGCTGCCCGGGTGAGGGCGGCAGCGCGGCCACCACGCCCAGCGCGGCCACCACGCCCAGCAT
  CACCGCCACGCCCAGCCCCCGCCGCCGGAGCAGCGCCGCTAGCGTGGGCCGCGCCGACGCCCCGGTTGCG
  CTGGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226356 representing NM_001122772
  Red=Cloning site Green=Tags(s)
  
  MSRGAGALQRRTTTYLISLTLVKLESVPPPPPSPSAAAAGAAGARGSETGDPGSPRGAEEPGKKRHERLF
  HRQDALWISTSSAGTGGAEPPALSPAPASPARPVSPAPGRRLSLWAVPPGPPLSGGLSPDPKPGGAPTSS
  RRPLLSSPSWGGPEPEGRAGGGIPGSSSPHPGTGSRRLKVAPPPPAPKPCKTVTTSGAKAGGGKGAGSRL
  SWPESEGKPRVKGSKSSAGTGASVSAAATAAAAGGGGSTASTSGGVGAGAGARGKLSPRKGKSKTLDNSD
  LHPGPPAGSPPPLTLPPTPSPATAVTAASAQPPGPAPPITLEPPAPGLKRGREGGRASTRDRKMLKFISG
  IFTKSTGGPPGSGPLPGPPSLSSGSGSRELLGAELRASPKAVINSQEWTLSRSIPELRLGVLGDARSGKS
  SLIHRFLTGSYQVLEKTESEQYKKEMLVDGQTHLVLIREEAGAPDAKFSGWADAVIFVFSLEDENSFQAV
  SRLHGQLSSLRGEGRGGLALALVGTQDRISASSPRVVGDARARALCADMKRCSYYETCATYGLNVDRVFQ
  EVAQKVVTLRKQQQLLAACKSLPSSPSHSAASTPVAGQASNGGHTSDYSSSLPSSPNVGHRELRAEAAAV
  AGLSTPGSLHRAAKRRTSLFANRRGSDSEKRSLDSRGETTGSGRAIPIKQSFLLKRSGNSLNKEWKKKYV
  TLSSNGFLLYHPSINDYIHSTHGKEMDLLRTTVKVPGKRPPRAISAFGPSASINGLVKDMSTVQMGEGLE
  ATTPMPSPSPSPSSLQPPPDQTSKHLLKPDRNLARALSTDCTPSGDLSPLSREPPPSPMVKKQRRKKLTT
  PSKTEGSAGQAEAKRKMWKLKSFGSLRNIYKAEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQIL
  ASLQCCESSKVKLRTDSQSEAVAIQAIRNAKGNSICVDCGAPNPTWASLNLGALICIECSGIHRNLGTHL
  SRVRSLDLDDWPRELTLVLTAIGNDTANRVWESDTRGRAKPSRDSSREERESWIRAKYEQLLFLAPLSTS
  EEPLGRQLWAAVQAQDVATVLLLLAHARHGPLDTSVEDPQLRSPLHLAAELAHVVITQLLLWYGADVAAR
  DAQGRTALFYARQAGSQLCADILLQHGCPGEGGSAATTPSAATTPSITATPSPRRRSSAASVGRADAPVA
  LV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001122772
• ORF Size: 3576 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001122772.1, NM_001122772.2, NP_001116244.1
• RefSeq ORF: 3579
• Locus ID: 116986
• Protein Pathways: Endocytosis
• MW: 124.4 kDa
• Gene Summary: The protein encoded by this gene belongs to the centaurin gamma-like family. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. It is overexpressed in cancer cells, and promotes cancer cell invasion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]