CrkRS (CDK12) (NM_015083) Human Tagged ORF Clone
CAT#: RC226399L3
- LentiORF®
Lenti ORF clone of Human cyclin-dependent kinase 12 (CDK12), transcript variant 2, Myc-DDK-tagged
"NM_015083" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | CrkRS |
Synonyms | CRK7; CRKR; CRKRS |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC226399).
|
Restriction Sites | AscI-MluI Cloning Scheme for this gene |
ACCN | NM_015083 |
ORF Size | 4443 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015083.1 |
RefSeq ORF | 4446 bp |
Locus ID | 51755 |
UniProt ID | Q9NYV4 |
Cytogenetics | 17q12 |
Protein Families | Druggable Genome, Protein Kinase |
MW | 163 kDa |
Gene Summary | Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC317169 | CDK12 (untagged)-Human cyclin-dependent kinase 12 (CDK12), transcript variant 2 |
USD 1,100.00 |
|
RC226399 | CDK12 (Myc-DDK-tagged)-Human cyclin-dependent kinase 12 (CDK12), transcript variant 2 |
USD 1,560.00 |
|
RG226399 | CDK12 (GFP-tagged) - Human cyclin-dependent kinase 12 (CDK12), transcript variant 2 |
USD 1,720.00 |
|
RC226399L1 | Lenti ORF clone of Human cyclin-dependent kinase 12 (CDK12), transcript variant 2, Myc-DDK-tagged |
USD 2,172.00 |
|
RC226399L2 | Lenti ORF clone of Human cyclin-dependent kinase 12 (CDK12), transcript variant 2, mGFP tagged |
USD 1,760.00 |
|
RC226399L4 | Lenti ORF clone of Human cyclin-dependent kinase 12 (CDK12), transcript variant 2, mGFP tagged |
USD 1,760.00 |
{0} Product Review(s)
Be the first one to submit a review