PCDH15 (NM_001142767) Human Tagged ORF Clone

CAT#: RC226748

PCDH15 (Myc-DDK-tagged)-Human protocadherin-related 15 (PCDH15), transcript variant F

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCDH15
• Synonyms: CDHR15; DFNB23; USH1F
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226748 representing NM_001142767
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTCGACAGTTTTATCTCTGGACATGTTTAGCTTCAGGGATCATCCTGGGCTCTCTCTTTGAAATCT
  GCTTGGGCCAGTATGATGATGATTGCAAACTAGCTAGGGGAGGACCACCAGCTACCATAGTTGCTATTGA
  TGAAGAAAGTCGGAATGGTACAATTCTGGTGGACAACATGCTGATCAAAGGGACTGCTGGAGGACCAGAC
  CCCACCATAGAACTTTCTTTAAAGGATAATGTGGATTACTGGGTGTTGATGGATCCTGTTAAGCAAATGC
  TTTTCCTGAACAGCACCGGAAGAGTTCTGGATAGAGATCCACCGATGAACATACACTCCATTGTGGTGCA
  GGTCCAGTGCATCAACAAAAAAGTGGGCACTATTATCTACCATGAAGTGCGAATAGTGGTGAGAGACAGG
  AATGACAACTCACCCACTTTCAAGCATGAAAGCTACTATGCCACAGTGAATGAGCTCACTCCAGTTGGTA
  CCACAATATTCACAGGATTTTCAGGAGACAATGGAGCTACAGATATAGATGATGGACCAAATGGACAGAT
  AGAGTATGTTATTCAGTATAATCCAGATGATCCGGACCGTGCCCAAAATCTGAATGAGAGGCGAACCACC
  ACCACCACTCTCACAGTGGATGTTCTGGATGGAGATGACTTGGGTCCAATGTTTCTTCCTTGTGTCCTTG
  TGCCAAACACTCGTGATTGCCGTCCACTCACTTATCAAGCTGCCATACCTGAGTTGAGAACTCCGGAAGA
  ACTGAACCCCATTATTGTTACGCCACCAATCCAAGCCATTGATCAGGACCGGAATATTCAACCGCCATCA
  GATAGGCCAGGAATCCTCTATTCCATCCTTGTTGGGACTCCTGAGGATTACCCACGATTTTTCCATATGC
  ATCCTAGGACAGCAGAACTTAGTCTCCTGGAGCCAGTAAACAGAGACTTTCACCAGAAATTTGATTTGGT
  TATTAAGGCTGAACAAGACAATGGTCATCCTCTTCCTGCCTTTGCCGGTCTACACATTGAAATACTGGAT
  GAAAACAATCAAAGTCCATATTTTACAATGCCCAGTTATCAAGGCTATATCCTGGAATCTGCCCCAGTGG
  GAGCAACCATTTCGGACAGTCTCAATTTGACTTCACCTTTAAGAATAGTAGCTCTGGACAAGGACATAGA
  AGATACAAAAGACCCAGAGCTTCACCTTTTTCTGAATGACTACACCTCAGTCTTCACCGTCACACAGACT
  GGTATTACTCGCTACCTCACCTTACTTCAACCAGTGGACAGGGAAGAACAGCAAACTTACACCTTTTCGA
  TAACAGCATTTGATGGTGTACAAGAAAGTGAGCCAGTCATCGTCAATATTCAAGTGATGGATGCAAATGA
  TAACACGCCAACCTTCCCTGAAATATCCTATGATGTGTATGTTTATACAGACATGAGACCTGGGGACAGT
  GTCATACAGCTCACTGCAGTCGACGCAGACGAAGGGTCAAATGGGGAGATCACATATGAAATCCTTGTTG
  GGGCTCAGGGAGACTTCATCATCAATAAAACAACAGGGCTTATCACCATCGCTCCAGGGGTGGAAATGAT
  AGTCGGGCGGACTTACGCACTCACGGTCCAAGCAGCGGATAATGCTCCTCCTGCAGAGCGAAGGAACTCC
  ATCTGCACTGTGTATATTGAAGTGCTTCCACCAAATAATCAAAGCCCTCCTCGCTTCCCACAGCTGATGT
  ATAGCCTTGAAATTAGTGAAGCCATGAGGGTTGGTGCTGTTTTATTAAATCTACAGGCAACTGATCGAGA
  GGGAGACTCAATAACATATGCCATTGAGAATGGAGATCCTCAGAGAGTTTTTAATCTTTCAGAAACCACG
  GGGATTCTAACCTTAGGGAAAGCACTGGACAGGGAAAGCACTGATCGCTACATTCTGATCATCACAGCTT
  CAGATGGCAGGCCAGATGGGACCTCAACTGCCACAGTAAACATAGTGGTGACAGATGTCAATGACAATGC
  TCCAGTGTTTGATCCTTATCTGCCAAGAAATTTATCTGTGGTGGAAGAAGAAGCCAATGCCTTTGTGGGT
  CAAGTAAAAGCAACAGACCCTGATGCTGGAATAAATGGTCAAGTGCACTACAGTTTGGGTAACTTTAATA
  ATCTTTTTCGTATCACATCCAATGGGAGCATTTACACAGCAGTGAAGCTTAACAGAGAAGTCAGGGACTA
  CTATGAACTTGTTGTTGTGGCAACAGATGGAGCAGTACACCCTCGTCATTCAACTCTAACCTTGGCCATC
  AAGGTTTTGGACATTGATGATAACAGTCCTGTGTTCACCAATTCAACATACACTGTCCTTGTTGAAGAGA
  ATTTGCCAGCTGGGACTACCATCCTTCAAATAGAGGCCAAAGATGTCGACCTTGGAGCAAATGTGTCTTA
  CCGGATAAGAAGCCCAGAAGTGAAGCACTTTTTTGCACTACATCCATTTACAGGAGAACTATCGCTTTTA
  AGGAGTTTAGATTATGAGGCATTTCCAGACCAAGAAGCAAGTATCACTTTTCTGGTAGAGGCCTTTGATA
  TTTATGGAACAATGCCACCTGGTATTGCTACTGTCACAGTGATTGTAAAGGATATGAATGATTATCCTCC
  TGTCTTTAGTAAACGAATATACAAAGGGATGGTGGCTCCGGATGCAGTCAAGGGTACACCTATCACAACA
  GTTTATGCTGAAGATGCAGACCCTCCTGGATTACCTGCAAGTCGTGTGAGGTATAGAGTAGATGATGTAC
  AGTTTCCTTACCCTGCCAGTATTTTTGAAGTGGAAGAAGATTCTGGAAGAGTAATAACACGAGTCAATCT
  TAATGAAGAACCTACAACAATTTTTAAGTTGGTGGTGGTTGCTTTTGATGATGGGGAGCCTGTGATGTCC
  AGCAGTGCCACAGTGAAGATTCTTGTCTTACATCCTGGTGAGATCCCACGCTTCACACAGGAGGAATATA
  GACCTCCTCCAGTAAGTGAACTTGCCACCAAAGGGACCATGGTTGGTGTAATTTCTGCTGCTGCCATTAA
  TCAAAGTATTGTGTACTCCATTGTTTCAGGAAATGAAGAAGATACATTTGGAATTAATAACATCACAGGT
  GTTATCTATGTGAATGGACCTCTGGATTATGAGACCAGGACAAGCTATGTACTTCGAGTCCAAGCTGATT
  CCCTGGAAGTGGTCCTTGCCAATCTCCGAGTTCCTTCAAAAAGCAATACAGCTAAAGTATACATTGAGAT
  TCAGGATGAAAATAATCATCCCCCAGTGTTTCAGAAAAAATTCTACATCGGAGGTGTATCTGAAGATGCA
  AGAATGTTTACTTCTGTACTCAGAGTGAAGGCTACTGATAAAGATACTGGCAATTATAGTGTCATGGCCT
  ACAGACTCATAATACCACCAATTAAAGAGGGAAAAGAAGGATTTGTAGTGGAAACATATACAGGGCTTAT
  CAAAACTGCTATGCTCTTCCATAATATGAGGAGATCCTACTTCAAGTTTCAAGTTATTGCAACTGACGAC
  TATGGGAAGGGACTGAGCGGCAAAGCCGATGTACTCGTCTCCGTGGTCAATCAGCTGGATATGCAAGTCA
  TTGTTTCCAATGTGCCTCCTACTCTAGTGGAAAAAAAGATAGAAGATCTTACAGAGATCTTGGATCGCTA
  TGTTCAGGAACAAATTCCTGGTGCCAAGGTCGTAGTGGAGTCCATTGGAGCTCGCCGGCATGGAGATGCC
  TTTTCCCTAGAAGATTACACCAAATGTGACTTGACTGTCTATGCAATTGACCCCCAAACCAACAGAGCCA
  TCGATAGAAATGAGCTTTTTAAATTTTTGGATGGCAAACTACTTGATATCAATAAAGACTTTCAGCCGTA
  TTATGGGGAAGGAGGACGCATTCTGGAGATCCGGACTCCAGAGGCAGTGACCAGCATTAAAAAGAGAGGA
  GAAAGTCTAGGATACACAGAAGGGGCCTTGTTGGCTCTGGCCTTCATCATCATCCTCTGCTGCATTCCTG
  CCATCTTGGTGGTTTTGGTCAGCTACAGACAACGTCAAGCTGAGTGTACAAAGACTGCACGAATTCAGGC
  CGCATTACCCGCGGCTAAACCAGCAGTGCCGGCTCCTGCACCAGTGGCAGCGCCCCCGCCGCCGCCGCCG
  CCTCCGCCAGGTGCGCATCTCTATGAAGAACTTGGAGACAGCTCAATTCTTTTCCTTCTCTACCATTTTC
  AACAAAGCAGGGGAAATAACTCAGTCTCAGAAGACAGGAAACATCAACAAGTTGTGATGCCCTTTTCTTC
  CAATACTATTGAGGCTCACAAGTCAGCTCATGTAGACGGATCACTTAAGAGCAACAAACTGAAGTCTGCA
  AGAAAATTCACATTTCTATCTGATGAGGATGACTTAAGTGCCCATAATCCCCTTTATAAGGAAAACATAA
  GTCAAGTATCAACAAATTCAGACATTTCACAGAGAACAGATTTTGTAGACCCATTTTCACCCAAAATACA
  AGCCAAGAGTAAGTCTCTGAGGGGCCCAAGAGAAAAGATTCAGAGGCTGTGGAGTCAGTCAGTCAGCTTA
  CCCAGGAGGCTGATGAGGAAAGTTCCAAATAGACCAGAGATCATAGATCTGCAGCAGTGGCAAGGCACCA
  GGCAGAAAGCTGAAAATGAAAACACTGGAATCTGTACAAACAAAAGAGGTAGCAGCAATCCATTGCTTAC
  AACTGAAGAGGCAAATTTGACAGAGAAAGAGGAAATAAGGCAAGGTGAAACACTGATGATAGAAGGAACA
  GAACAGTTGAAATCTCTCTCTTCAGACTCTTCATTTTGCTTTCCCAGGCCTCACTTCTCATTCTCCACTT
  TGCCAACTGTTTCAAGAACTGTGGAACTCAAATCAGAACCTAATGTCATCAGTTCTCCTGCTGAGTGTTC
  CTTGGAACTTTCTCCTTCAAGGCCTTGTGTTTTACATTCTTCACTCTCTAGGAGAGAGACACCTATTTGT
  ATGTTACCTATTGAAACCGAAAGAAATATTTTTGAAAATTTTGCCCATCCACCAAACATCTCTCCTTCTG
  CCTGTCCCCTTCCCCCTCCTCCTCCTATTTCTCCTCCTTCTCCTCCTCCTGCTCCTGCTCCTCTTGCTCC
  TCCTCCTGACATTTCTCCTTTTTCTCTTTTTTGTCCTCCTCCCTCTCCTCCTTCTATCCCTCTTCCTCTT
  CCTCCTCCTACATTTTTTCCACTTTCCGTTTCAACGTCTGGTCCCCCAACACCACCTCTTCTACCTCCAT
  TTCCAACTCCTCTTCCTCCACCACCTCCTTCTATTCCTTGCCCTCCACCTCCTTCAGCTTCATTTCTGTC
  CACAGAGTGTGTCTGTATAACAGGTGTTAAATGCACGACCAACTTGATGCCTGCCGAGAAAATTAAGTCC
  TCTATGACACAGCTATCAACAACGACAGTGTGTAAAACAGACCCTCAGAGAGAACCAAAAGGCATCCTCA
  GACACGTTAAAAACTTAGCAGAACTTGAAAAATCAGTAGCTAACATGTACAGTCAAATAGAAAAAAACTA
  TCTACGCACAAATGTTTCAGAACTTCAAACTATGTGCCCTTCAGAAGTAACAAATATGGAAATCACATCT
  GAACAAAACAAGGGGAGTTTGAACAATATTGTCGAGGGAACTGAAAAACAATCTCACAGTCAATCTACTT
  CACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226748 representing NM_001142767
  Red=Cloning site Green=Tags(s)
  
  MFRQFYLWTCLASGIILGSLFEICLGQYDDDCKLARGGPPATIVAIDEESRNGTILVDNMLIKGTAGGPD
  PTIELSLKDNVDYWVLMDPVKQMLFLNSTGRVLDRDPPMNIHSIVVQVQCINKKVGTIIYHEVRIVVRDR
  NDNSPTFKHESYYATVNELTPVGTTIFTGFSGDNGATDIDDGPNGQIEYVIQYNPDDPDRAQNLNERRTT
  TTTLTVDVLDGDDLGPMFLPCVLVPNTRDCRPLTYQAAIPELRTPEELNPIIVTPPIQAIDQDRNIQPPS
  DRPGILYSILVGTPEDYPRFFHMHPRTAELSLLEPVNRDFHQKFDLVIKAEQDNGHPLPAFAGLHIEILD
  ENNQSPYFTMPSYQGYILESAPVGATISDSLNLTSPLRIVALDKDIEDTKDPELHLFLNDYTSVFTVTQT
  GITRYLTLLQPVDREEQQTYTFSITAFDGVQESEPVIVNIQVMDANDNTPTFPEISYDVYVYTDMRPGDS
  VIQLTAVDADEGSNGEITYEILVGAQGDFIINKTTGLITIAPGVEMIVGRTYALTVQAADNAPPAERRNS
  ICTVYIEVLPPNNQSPPRFPQLMYSLEISEAMRVGAVLLNLQATDREGDSITYAIENGDPQRVFNLSETT
  GILTLGKALDRESTDRYILIITASDGRPDGTSTATVNIVVTDVNDNAPVFDPYLPRNLSVVEEEANAFVG
  QVKATDPDAGINGQVHYSLGNFNNLFRITSNGSIYTAVKLNREVRDYYELVVVATDGAVHPRHSTLTLAI
  KVLDIDDNSPVFTNSTYTVLVEENLPAGTTILQIEAKDVDLGANVSYRIRSPEVKHFFALHPFTGELSLL
  RSLDYEAFPDQEASITFLVEAFDIYGTMPPGIATVTVIVKDMNDYPPVFSKRIYKGMVAPDAVKGTPITT
  VYAEDADPPGLPASRVRYRVDDVQFPYPASIFEVEEDSGRVITRVNLNEEPTTIFKLVVVAFDDGEPVMS
  SSATVKILVLHPGEIPRFTQEEYRPPPVSELATKGTMVGVISAAAINQSIVYSIVSGNEEDTFGINNITG
  VIYVNGPLDYETRTSYVLRVQADSLEVVLANLRVPSKSNTAKVYIEIQDENNHPPVFQKKFYIGGVSEDA
  RMFTSVLRVKATDKDTGNYSVMAYRLIIPPIKEGKEGFVVETYTGLIKTAMLFHNMRRSYFKFQVIATDD
  YGKGLSGKADVLVSVVNQLDMQVIVSNVPPTLVEKKIEDLTEILDRYVQEQIPGAKVVVESIGARRHGDA
  FSLEDYTKCDLTVYAIDPQTNRAIDRNELFKFLDGKLLDINKDFQPYYGEGGRILEIRTPEAVTSIKKRG
  ESLGYTEGALLALAFIIILCCIPAILVVLVSYRQRQAECTKTARIQAALPAAKPAVPAPAPVAAPPPPPP
  PPPGAHLYEELGDSSILFLLYHFQQSRGNNSVSEDRKHQQVVMPFSSNTIEAHKSAHVDGSLKSNKLKSA
  RKFTFLSDEDDLSAHNPLYKENISQVSTNSDISQRTDFVDPFSPKIQAKSKSLRGPREKIQRLWSQSVSL
  PRRLMRKVPNRPEIIDLQQWQGTRQKAENENTGICTNKRGSSNPLLTTEEANLTEKEEIRQGETLMIEGT
  EQLKSLSSDSSFCFPRPHFSFSTLPTVSRTVELKSEPNVISSPAECSLELSPSRPCVLHSSLSRRETPIC
  MLPIETERNIFENFAHPPNISPSACPLPPPPPISPPSPPPAPAPLAPPPDISPFSLFCPPPSPPSIPLPL
  PPPTFFPLSVSTSGPPTPPLLPPFPTPLPPPPPSIPCPPPPSASFLSTECVCITGVKCTTNLMPAEKIKS
  SMTQLSTTTVCKTDPQREPKGILRHVKNLAELEKSVANMYSQIEKNYLRTNVSELQTMCPSEVTNMEITS
  EQNKGSLNNIVEGTEKQSHSQSTSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001142767
• ORF Size: 5745 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001142767.1, NP_001136239.1
• RefSeq ORF: 5748
• Locus ID: 65217
• Protein Families: Druggable Genome, Transmembrane
• MW: 211.32 kDa
• Gene Summary: This gene is a member of the cadherin superfamily. Family members encode integral membrane proteins that mediate calcium-dependent cell-cell adhesion. It plays an essential role in maintenance of normal retinal and cochlear function. Mutations in this gene result in hearing loss and Usher Syndrome Type IF (USH1F). Extensive alternative splicing resulting in multiple isoforms has been observed in the mouse ortholog. Similar alternatively spliced transcripts are inferred to occur in human, and additional variants are likely to occur. [provided by RefSeq, Dec 2008]