Apc5 (ANAPC5) (NM_001137559) Human Tagged ORF Clone

CAT#: RC226883

ANAPC5 (Myc-DDK-tagged)-Human anaphase promoting complex subunit 5 (ANAPC5), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ANAPC5
• Synonyms: APC5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226883 representing NM_001137559
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGAAGGCGAGTTGAAGGATATGGAACAGTTTTTTGATGACCTTTCAGATTCTTTCTCTGGAACTG
  AACCAGAGGTTCACAAAACAAGTGTAGTAGGTTTGTTTCTGCGTCACATGATCTTGGCCTACAGTAAGCT
  TTCTTTCAGCCAAGTGTTTAAACTGTACACTGCCCTTCAGCAGTACTTCCAGAATGGTGAGAAAAAGACA
  GTGGAGGATGCTGATATGGAACTGACCAGTAGAGATGAGGGTGAAAGAAAAATGGAAAAAGAAGAACTTG
  ATGTATCTGTAAGAGAAGAGGAGGTATCTTGCAGTGGGCCTCTGTCCCAAAAACAAGCAGAATTTTTTCT
  TTCTCAACAGGCTTCTTTGCTAAAGAATGATGAGACTAAGGCCCTCACTCCAGCTTCCTTGCAGAAGGAA
  TTAAACAATTTGTTGAAATTTAATCCTGATTTTGCTGAAGCGCATTATCTCAGCTACTTAAACAACCTCC
  GTGTCCAAGATGTTTTCAGTTCAACACACAGTCTCCTCCATTATTTTGATCGTCTGATTCTTACCGGAGC
  CGAAAGCAAAAGTAATGGGGAAGAGGGCTATGGCCGGAGCTTGAGATACGCCGCTCTGAATCTTGCCGCC
  CTGCACTGCCGCTTCGGTCACTATCAACAGGCAGAGCTCGCCCTGCAGGAGGCAATTAGGATTGCCCAGG
  AGTCCAACGATCACGTGTGTCTCCAGCACTGTTTGAGCTGGCTTTATGTGCTGGGGCAGAAGAGATCCGA
  TAGCTATGTTCTGCTGGAGCATTCTGTGAAGAAGGCAGTACATTTTGGGTTACCGAGAGCTTTTGCTGGG
  AAGACGGCAAACAAGCTGATGGATGCCCTAAAGGACTCCGACCTCCTGCACTGGAAACACAGCCTGTCAG
  AGCTCATCGATATCAGCATCGCACAGAAAACGGCCATCTGGAGGCTGTATGGCCGCAGCACCATGGCACT
  GCAACAGGCCCAGATGTTGCTGAGCATGAACAGCCTGGAGGCGGTGAATGCGGGCGTGCAGCAGAACAAC
  ACAGAGTCCTTTGCTGTCGCACTCTGCCACCTCGCAGAGCTACACGCGGAGCAGGGCTGTTTTGCTGCAG
  CTTCTGAAGTGTTAAAGCACTTGAAGGAACGATTTCCGCCTAATAGTCAGCACGCCCAGTTATGGATGCT
  ATGTGATCAAAAAATACAGTTTGACAGAGCAATGAATGATGGCAAATATCATTTGGCTGATTCACTTGTT
  ACAGGAATCACAGCTCTCAATAGCATAGAGGGTGTTTATAGGAAAGCGGTTGTATTACAAGCTCAGAACC
  AAATGTCAGAGGCACATAAGCTTTTACAAAAATTGTTGGTTCATTGTCAGAAACTGAAGAACACAGAAAT
  GGTGATCAGTGTCCTACTGTCCGTGGCAGAGCTGTACTGGCGATCTTCCTCCCCTACCATCGCGCTGCCC
  ATGCTCCTGCAGGCTCTGGCCCTCTCCAAGGAGTACCGGTTACAGTACTTGGCCTCTGAAACAGTGCTGA
  ACTTGGCTTTTGCGCAGCTCATTCTTGGAATCCCAGAACAGGCCTTAAGTCTTCTCCACATGGCCATCGA
  GCCCATCTTGGCTGACGGGGCTATCCTGGACAAAGGTCGTGCCATGTTCTTAGTGGCCAAGTGCCAGGTG
  GCTTCAGCAGCTTCCTACGATCAGCCGAAGAAAGCAGAAGCTCTGGAGGCTGCCATCGAGAACCTCAATG
  AAGCCAAGAACTATTTTGCAAAGGTTGACTGCAAAGAGCGCATCAGGGACGTCGTTTACTTCCAGGCCAG
  ACTCTACCATACCCTGGGGAAGACCCAGGAGAGGAACCGGTGTGCGATGCTCTTCCGGCAGCTGCATCAG
  GAGCTGCCCTCTCATGGGGTACCCTTGATAAACCATCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226883 representing NM_001137559
  Red=Cloning site Green=Tags(s)
  
  MAEGELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSFSQVFKLYTALQQYFQNGEKKT
  VEDADMELTSRDEGERKMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQQASLLKNDETKALTPASLQKE
  LNNLLKFNPDFAEAHYLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAESKSNGEEGYGRSLRYAALNLAA
  LHCRFGHYQQAELALQEAIRIAQESNDHVCLQHCLSWLYVLGQKRSDSYVLLEHSVKKAVHFGLPRAFAG
  KTANKLMDALKDSDLLHWKHSLSELIDISIAQKTAIWRLYGRSTMALQQAQMLLSMNSLEAVNAGVQQNN
  TESFAVALCHLAELHAEQGCFAAASEVLKHLKERFPPNSQHAQLWMLCDQKIQFDRAMNDGKYHLADSLV
  TGITALNSIEGVYRKAVVLQAQNQMSEAHKLLQKLLVHCQKLKNTEMVISVLLSVAELYWRSSSPTIALP
  MLLQALALSKEYRLQYLASETVLNLAFAQLILGIPEQALSLLHMAIEPILADGAILDKGRAMFLVAKCQV
  ASAASYDQPKKAEALEAAIENLNEAKNYFAKVDCKERIRDVVYFQARLYHTLGKTQERNRCAMLFRQLHQ
  ELPSHGVPLINHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001137559
• ORF Size: 1929 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001137559.1, NP_001131031.1
• RefSeq ORF: 1932
• Locus ID: 51433
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis
• MW: 72.3 kDa
• Gene Summary: This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a. [provided by RefSeq, Nov 2008]