DOLPP1 (NM_001135917) Human Tagged ORF Clone

CAT#: RC226887

DOLPP1 (Myc-DDK-tagged)-Human dolichyl pyrophosphate phosphatase 1 (DOLPP1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY427729) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226887 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DOLPP1
• Synonyms: LSFR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226887 representing NM_001135917
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGGACGGACAGTGCTCGCTCCCCGCTTCATGGCGGCCGGTGACCCTCACCCACGTCGAATATC
  CTGCAGGTGATCTCTCTGGCCACCTCCTTGCCTACCTGAGCCTCAGCCCTGTATTTGTCATCGTCGGTTT
  CGTGACCCTCATCATATTTAAGCGGGAGCTGCACACGATCTCCTTCCTTGGGGGCCTGGCACTGAACGAG
  GGGGTCAACTGGCTGATCAAAAACGTCATCCAGGAGCCACGGCCCTGTGGAGGCCCCCACACAGCAGTGG
  GCACCAAGTACGGGATGCCCTCCAGCCATTCCCAGTTTATGTGGTTCTTCTCCGTCTATTCCTTCCTTTT
  CCTGTATTTAAGAATGCACCAAACAAACAACGCCAGGTTCCTGGACTTGCTGTGGAGGCACGTGCTCTCC
  CTGGGACTCCTCGCTGTGGCCTTCCTAGTCTCCTACAGCAGGCCTGTCTCCGAGTTCTTCCTAATCCGAG
  ACACAAGCCTCATTCCCAACGTACTCTGGTTTGAGTACACGGTAACCCGGGCAGAAGCCAGGAACAGACA
  ACGCAAGCTGGGGACGAAACTGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226887 representing NM_001135917
  Red=Cloning site Green=Tags(s)
  
  MAADGQCSLPASWRPVTLTHVEYPAGDLSGHLLAYLSLSPVFVIVGFVTLIIFKRELHTISFLGGLALNE
  GVNWLIKNVIQEPRPCGGPHTAVGTKYGMPSSHSQFMWFFSVYSFLFLYLRMHQTNNARFLDLLWRHVLS
  LGLLAVAFLVSYSRPVSEFFLIRDTSLIPNVLWFEYTVTRAEARNRQRKLGTKLQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001135917
• ORF Size: 585 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001135917.1, NP_001129389.1
• RefSeq ORF: 588
• Locus ID: 57171
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: N-Glycan biosynthesis
• MW: 22 kDa
• Gene Summary: A similar gene has been characterized in mice and encodes dolichyl pyrophosphate (Dol-P-P) phosphatase. This protein dephosphorylates dolichyl pyrophosphate so that it may be re-utilized as a glycosyl carrier lipid by the oligosaccharyltransferase multisubunit complex in the ER. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jun 2012]