DDX4 (NM_001136034) Human Tagged ORF Clone

CAT#: RC226957

DDX4 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 (DDX4), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY427781) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226957 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DDX4 protein (Cat# TP326957). The protein was produced from HEK293T cells transfected with DDX4 cDNA clone (Cat# RC226957) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DDX4
• Synonyms: DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4; MGC111074; OTTHUMP00000122546; VASA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC226957 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGATGAAGATTGGGAAGCAGAAATCAACCCTCATATGTCTTCCTATGTTCCCATATTTGAGAAGG
  ATAGGTATTCTGGAGAAAATGGAGACAATTTTAACAGGACTCCAGCTTCATCATCAGAAATGGATGATGG
  ACCTTCTCGAAGAGATCATTTCATGAAAAGTGGATTTGCCTCTGGGCGGAATTTTGGAAACAGAGATGCT
  GGTGAGTGTAATAAGCGAGATAATACATCCACAATGGGTGGTTTTGGAGTTGGAAAGAGTTTTGGAAACA
  GAGGTTTTTCAAACAGCAGGTTTGAAGATGGTGATAGCTCTGGTTTCTGGAGAGAGTCTAGTAATGACTG
  CGAAGATAATCCAACACGGAACAGAGGGTTTTCCAAGAGAGGCGGCTATCGAGATGGAAATAATTCAGAA
  GCTTCAGGGCCATACAGAAGAGGTGGAAGAGGTAGTTTCCGAGGTTGCCGTGGAGGATTTGGTCTAGGAA
  GTCCAAATAATGACTTAGACCCAGACGAATGTATGCAGCGCACTGGTGGCCTTTTTGGTTCTAGAAGACC
  AGTATTAAGTGGCACAGGTAATGGTGATACTTCTCAAAGCAGAAGTGGCAGTGGAAGTGAACGAGGTGGT
  TACAAAGGTTTAAATGAAGAAGTAATAACAGGCTCTGGAAAGAATTCTTGGAAGTCAGAAGCAGAAGGAG
  GAGAAAGTAGTGATACTCAAGGACCAAAAGTGACCTACATACCCCCTCCTCCACCTGAGGATGAGGACTC
  CATCTTTGCACATTATCAGACAGGCATAAACTTCGACAAATACGACACTATTCTTGTGGAAGTGTCTGGA
  CATGATGCACCACCAGCAATTCTGACTTTTGAAGAAGCTAATCTCTGTCAGACACTGAATAACAACATTG
  CTAAAGCTGGTTATACTAAGCTTACTCCTGTGCAAAAATACAGTATTCCTATCATACTTGCAGGACGAGA
  TTTGATGGCTTGCGCTCAAACAGGGTCTGGGAAGACTGCGGCTTTTCTCCTACCAATTTTGGCTCATATG
  ATGCATGATGGAATAACTGCCAGTCGTTTTAAAGAGTTGCAGGAACCAGAGTGTATTATTGTAGCACCAA
  CTCGAGAATTGGTCAACCAGATTTATTTGGAAGCCAGAAAATTTTCTTTTGGGACTTGTGTAAGAGCTGT
  TGTTATATATGGGGGAACCCAGCTGGGACATTCAATTCGACAAATAGTACAAGGCTGTAATATATTATGT
  GCTACTCCTGGAAGACTGATGGATATCATAGGCAAAGAAAAGATTGGTCTCAAACAGATCAAATACTTAG
  TTTTGGATGAAGCTGATCGCATGTTGGATATGGGTTTTGGTCCAGAAATGAAGAAGTTAATTTCTTGCCC
  AGGAATGCCATCAAAGGAACAGCGCCAAACCCTTATGTTCAGTGCAACTTTTCCAGAGGAAATTCAAAGG
  TTGGCTGCAGAGTTTTTAAAGTCAAATTATCTGTTTGTTGCTGTTGGACAAGTGGGTGGAGCATGTAGAG
  ATGTTCAGCAGACCGTTCTCCAAGTTGGCCAGTTCTCAAAAAGAGAAAAGCTCGTTGAAATTCTGCGAAA
  CATAGGGGATGAAAGAACTATGGTCTTTGTTGAAACTAAGAAAAAAGCAGATTTTATTGCAACTTTTCTT
  TGTCAAGAAAAAATATCAACTACAAGTATTCATGGTGATCGGGAACAGAGAGAGCGGGAGCAAGCTCTTG
  GAGATTTTCGCTTTGGAAAGTGCCCAGTTCTTGTTGCTACTTCAGTAGCTGCCAGAGGGCTGGATATTGA
  AAATGTGCAACATGTTATCAATTTTGATCTTCCTTCTACCATTGATGAATATGTTCATCGAATTGGGCGT
  ACTGGTCGTTGTGGGAATACTGGCAGAGCAATTTCCTTTTTTGATCTTGAATCGGATAACCATTTAGCAC
  AGCCTCTAGTAAAAGTATTGACAGATGCTCAACAGGATGTTCCTGCATGGTTGGAAGAAATTGCCTTTAG
  TACATACATTCCTGGCTTCAGTGGTAGTACAAGAGGAAACGTGTTTGCATCAGTTGATACCAGAAAGGGC
  AAGAGCACTTTGAACACAGCTGGGTTTTCTTCTTCACAAGCTCCCAATCCAGTAGATGATGAGTCATGGG
  AT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC226957 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGDEDWEAEINPHMSSYVPIFEKDRYSGENGDNFNRTPASSSEMDDGPSRRDHFMKSGFASGRNFGNRDA
  GECNKRDNTSTMGGFGVGKSFGNRGFSNSRFEDGDSSGFWRESSNDCEDNPTRNRGFSKRGGYRDGNNSE
  ASGPYRRGGRGSFRGCRGGFGLGSPNNDLDPDECMQRTGGLFGSRRPVLSGTGNGDTSQSRSGSGSERGG
  YKGLNEEVITGSGKNSWKSEAEGGESSDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSG
  HDAPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIILAGRDLMACAQTGSGKTAAFLLPILAHM
  MHDGITASRFKELQEPECIIVAPTRELVNQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILC
  ATPGRLMDIIGKEKIGLKQIKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQR
  LAAEFLKSNYLFVAVGQVGGACRDVQQTVLQVGQFSKREKLVEILRNIGDERTMVFVETKKKADFIATFL
  CQEKISTTSIHGDREQREREQALGDFRFGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGR
  TGRCGNTGRAISFFDLESDNHLAQPLVKVLTDAQQDVPAWLEEIAFSTYIPGFSGSTRGNVFASVDTRKG
  KSTLNTAGFSSSQAPNPVDDESWD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001136034
• ORF Size: 2172 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001136034.1, NP_001129506.1
• RefSeq Size: 2880 bp
• RefSeq ORF: 2174 bp
• Locus ID: 54514
• MW: 79.3 kDa
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]