TPCN1 (NM_001143819) Human Tagged ORF Clone

CAT#: RC227015

TPCN1 (Myc-DDK-tagged)-Human two pore segment channel 1 (TPCN1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY428364) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227015 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TPCN1
• Synonyms: TPC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227015 representing NM_001143819
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTCTTGCTACATTGCCCAGGCTGGTCTTGAACTGCTAGGCTCAAGCAGTTCTCCCACCTTGACCT
  CCCAAAGCGCTGAGATTACAGAAGATGCCTCTAATGGAGGAGTTTCTGAGCAGCACCCCTGGCCCAGTGG
  CTTTGAAAGGGAGCTCAAACCAGAGACTATTTCAAGCCCTGGATATCATATCCTGAGGGCCACAGGAGAA
  GAGAACATGGCTGTGAGTTTGGATGACGACGTGCCGCTCATCCTGACCTTGGATGAGGGTGGCAGTGCCC
  CACTGGCTCCCTCCAACGGCCTGGGCCAAGAAGAGCTACCTAGCAAAAATGGCGGCAGCTATGCCATCCA
  CGACTCCCAGGCCCCCAGTCTCAGCTCTGGGGGTGAGAGTTCCCCCTCCAGCCCCGCACACAACTGGGAG
  ATGAATTACCAAGAGGCAGCAATCTACCTCCAGGAAGGCGAGAACAACGACAAGTTCTTCACCCACCCCA
  AGGATGCCAAGGCGCTGGCGGCCTACCTCTTTGCACACAATCACCTCTTCTACCTGATGGAGCTGGCCAC
  GGCCCTGCTGCTGCTGCTGCTCTCCCTGTGCGAGGCCCCCGCCGTCCCCGCACTCCGGCTTGGCATCTAT
  GTCCACGCCACCCTGGAGCTGTTTGCCCTGATGGTGGTAGTGTTTGAACTCTGCATGAAGTTACGCTGGC
  TGGGCCTCCACACCTTCATCCGGCACAAGCGGACCATGGTCAAGACCTCGGTGCTGGTGGTGCAGTTTGT
  CGAGGCCATCGTGGTGTTGGTACGGCAGATGTCCCATGTGCGGGTGACCCGAGCACTGCGCTGCATTTTC
  CTGGTGGACTGTCGGTATTGCGGTGGCGTCCGGCGCAACCTGCGGCAGATCTTCCAGTCCCTGCCGCCCT
  TCATGGACATCCTCCTGCTGCTGCTGTTCTTCATGATCATCTTTGCCATCCTCGGTTTCTACTTGTTCTC
  CCCTAACCCTTCAGACCCCTACTTCAGCACCCTGGAGAACAGCATCGTCAGTCTGTTTGTCCTTCTGACC
  ACAGCCAATTTCCCAGATGTGATGATGCCCTCCTACTCCCGGAACCCCTGGTCCTGCGTCTTCTTCATCG
  TGTACCTCTCCATCGAGCTGTATTTCATCATGAACCTGCTTCTGGCTGTGGTGTTCGACACCTTCAATGA
  CATTGAGAAACGCAAGTTCAAGTCTTTGCTACTGCACAAGCGAACCGCTATCCAGCATGCCTACCGCCTG
  CTCATCAGCCAGAGGAGGCCTGCCGGCATCTCCTACAGGCAGTTTGAAGGCCTCATGCGCTTCTACAAGC
  CCCGGATGAGTGCCAGGGAGCGCTATCTTACCTTCAAGGCCCTGAATCAGAACAACACACCCCTGCTCAG
  CCTAAAGGACTTTTACGATATCTACGAAGTTGCTGCTTTGAAGTGGAAGGCCAAGAAAAACAGAGAGCAC
  TGGTTTGATGAGCTTCCCAGGACGGCGCTCCTCATCTTCAAAGGTATTAATATCCTTGTGAAGTCCAAGG
  CCTTCCAGTATTTCATGTACTTGGTGGTGGCAGTCAACGGGGTCTGGATCCTCGTGGAGACATTTATGCT
  GAAAGGTGGGAACTTCTTCTCCAAGCACGTGCCCTGGAGTTACCTCGTCTTTCTAACTATCTATGGGGTG
  GAGCTGTTCCTGAAGGTTGCCGGCCTGGGCCCTGTGGAGTACTTGTCTTCCGGATGGAACTTGTTTGACT
  TCTCCGTGACAGTGTTCGCCTTCCTGGGACTGCTGGCGCTGGCCCTCAACATGGAGCCCTTCTATTTCAT
  CGTGGTCCTGCGCCCCCTCCAGCTGCTGAGGTTGTTTAAGTTGAAGGAGCGCTACCGCAACGTGCTGGAC
  ACCATGTTCGAGCTGCTGCCCCGGATGGCCAGCCTGGGCCTCACCCTGCTCATCTTTTACTACTCCTTCG
  CCATCGTGGGCATGGAGTTCTTCTGCGGGATCGTCTTCCCCAACTGCTGCAACACGAGTACAGTGGCAGA
  TGCCTACCGCTGGCGCAACCACACCGTGGGCAACAGGACCGTGGTGGAGGAAGGCTACTATTATCTCAAT
  AATTTTGACAACATCCTCAACAGCTTTGTGACCCTGTTTGAGCTCACAGTTGTCAACAACTGGTACATCA
  TCATGGAAGGCGTCACCTCTCAGACCTCCCACTGGAGCCGCCTCTACTTCATGACCTTTTACATTGTGAC
  CATGGTGGTGATGACGATCATTGTCGCCTTTATCCTCGAGGCCTTCGTCTTCCGAATGAACTACAGCCGC
  AAGAACCAGGACTCGGAAGTTGATGGTGGCATCACCCTTGAGAAGGAAATCTCCAAAGAAGAGCTGGTTG
  CCGTCCTGGAGCTCTACCGGGAGGCACGGGGGGCCTCCTCGGATGTCACCAGGCTGCTGGAGACCCTCTC
  CCAGATGGAGAGATACCAGCAACATTCCATGGTGTTTCTGGGACGGCGATCAAGGACCAAGAGCGACCTG
  AGCCTGAAGATGTACCAGGAGGAGATCCAGGAGTGGTATGAGGAGCATGCCAGGGAGCAAGAGCAGCAGC
  GACAACTCAGCAGCAGTGCAGCCCCCGCCGCCCAGCAGCCCCCAGGCAGCCGCCAGCGCTCCCAGACCGT
  TACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227015 representing NM_001143819
  Red=Cloning site Green=Tags(s)
  
  MESCYIAQAGLELLGSSSSPTLTSQSAEITEDASNGGVSEQHPWPSGFERELKPETISSPGYHILRATGE
  ENMAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAIHDSQAPSLSSGGESSPSSPAHNWE
  MNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIY
  VHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIF
  LVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLT
  TANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRL
  LISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREH
  WFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGV
  ELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLD
  TMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLN
  NFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSR
  KNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDL
  SLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143819
• ORF Size: 2664 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001143819.1, NM_001143819.2, NP_001137291.1
• RefSeq ORF: 2667
• Locus ID: 53373
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• MW: 101.6 kDa
• Gene Summary: Voltage-gated Ca(2+) and Na+ channels have 4 homologous domains, each containing 6 transmembrane segments, S1 to S6. TPCN1 is similar to these channels, but it has only 2 domains containing S1 to S6 (Ishibashi et al., 2000 [PubMed 10753632]). [supplied by OMIM, Mar 2008]