CCDC154 (NM_001143980) Human Tagged ORF Clone

CAT#: RC227147

CCDC154 (Myc-DDK-tagged)-Human coiled-coil domain containing 154 (CCDC154)

Product Images

Western blot validation of overexpression lysate (Cat# LY428440) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227147 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCDC154
• Synonyms: C16orf29
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227147 representing NM_001143980
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGAGTTGGCTGACAGTGGACCCTCAGGGGCATCGGCCCCTTCCCAGCTGAGAGCCGTCACCCTGG
  AAGACCTGGGGCTCCTCCTGGCAGGAGGCCTGGCCAGCCCCGAGCCCTTGAGCCTGGAGGAGCTCTCGGA
  GAGGTATGAGTCCAGCCATCCGACATCCACGGCCTCTGTCCCCGAGCAGGACACCGCCAAGCACTGGAAC
  CAGCTGGAGCAGTGGGTGGTGGAGCTGCAGGCCGAGGTGGCCTGCCTGCGGGAGCACAAGCAGCGCTGTG
  AGCGCGCCACGCGGAGCCTGCTGCGGGAGCTGCTCCAGGTGCGGGCCCGCGTGCAGCTGCAGGGCTCAGA
  GCTGAGGCAGTTGCAGCAGGAGGCGCGGCCGGCAGCCCAGGCCCCCGAAAAGGAGGCGCCGGAGTTCTCT
  GGTCTCCAGAACCAGATGCAGGCCCTGGACAAAAGGCTGGTGGAGGTCCGGGAAGCCTTGACCCGGCTCA
  GGAGGAGGCAGGTGCAACAGGAGGCGGAGAGAAGGGGCGCCGAGCAAGAGGCCGGCCTCAGACTGGCCAA
  GCTGACCGACTTGCTGCAGCAGGAGGAGCAGGGCCGGGAGGTGGCCTGCGGCGCCCTGCAGAAGAACCAA
  GAGGACAGCAGCCGGAGGGTGGACCTGGAGGTGGCCAGAATGCAGGCCCAGGTGACCAAGCTCGGCGAAG
  AGGTGAGCCTGCGCTTCCTGAAGAGGGAGGCCAAGCTGTGCGGCTTCCTGCAGAAGAGCTTCCTGGCCCT
  GGAGAAGAGAATGAAGGCCTCGGAGAGCTCACGGCTGAAGCTGGAGGGCAGCCTGCGGGGCGAGCTGGAG
  AGCCGGTGGGAGAAGCTTCGGGGGCTGATGGAGGAGCGCCTGCGGGCCCTGCAGGGGCAGCATGAGGAGA
  GCCACCTCCTGGAGCAGTGCCAGGGCCTGGATGCTGCCGTGGCCCAGCTGACCAAGTTTGTGCAGCAGAA
  CCAGGCGTCGCTGAACCGTGTCCTACTGGCCGAGGAGAAAGCCTGGGACGCCAAGGGGCGCTTGGAGGAA
  AGCCGGGCTGGGGAGCTGGCCGCCTATGTGCAGGAGAACCTGGAGGCCGCACAGCTGGCTGGCGAGCTGG
  CCCGGCAGGAGATGCATGGAGAGCTGGTGCTGCTCCGAGAGAAGAGCCGGGCTCTGGAGGCATCCGTGGC
  GCAGCTGGCCGGGCAGTTAAAGGAGCTGAGTGGCCATCTCCCGGCTCTGAGCAGCCGGCTCGATCTGCAG
  GAGCAGATGCTGGGCCTCAGGCTGTCCGAGGCAAAGACCGAATGGGAAGGTGCAGAGAGGAAGTCCCTGG
  AGGACCTGGCCAGGTGGCGGAAGGAGGTGACCGAACACCTGCGAGGGGTGCGGGAGAAGGTGGATGGCCT
  CCCCCAGCAGATAGAGAGTGTCTCCGACAAGTGCCTGCTTCATAAGAGCGACTCAGACCTCAGGATTTCC
  GCCGAAGGCAAGGCCAGGGAGTTCAAGGTTGGGGCCCTGCGGCAGGAGCTGGCCACGCTACTATCATCCG
  TGCAGCTGCTAAAGGAAGACAACCCTGGGCGGAAGATCGCGGAGATGCAGGGCAAGCTGGCCACGTTTCA
  GAACCAAATAATGAAGCTGGAAAACTGCGTCCAGGCCAACAAGACCATCCAGAACCTCAGGTTCAACACT
  GAGGCCCGGCTGCGCACGCAGGAGATGGCCACCCTGTGGGAGAGTGTGCTCCGGCTGTGGAGTGAGGAGG
  GCCCGCGGACGCCGCTGGGCAGCTGGAAGGCGCTCCCATCCCTGGTGAGGCCGCGGGTCTTCATCAAGGA
  CATGGCGCCTGGCAAGGTGGTGCCCATGAACTGCTGGGGCGTGTACCAGGCTGTGAGGTGGCTGCGCTGG
  AAGGCGTCCCTCATAAAGCTCAGGGCCCTGCGGAGGCCAGGAGGGGTCCTGGAGAAGCCCCACAGCCAGG
  AGCAGGTGCAGCAGCTCACACCCTCACTGTTTATCCAGAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227147 representing NM_001143980
  Red=Cloning site Green=Tags(s)
  
  MSELADSGPSGASAPSQLRAVTLEDLGLLLAGGLASPEPLSLEELSERYESSHPTSTASVPEQDTAKHWN
  QLEQWVVELQAEVACLREHKQRCERATRSLLRELLQVRARVQLQGSELRQLQQEARPAAQAPEKEAPEFS
  GLQNQMQALDKRLVEVREALTRLRRRQVQQEAERRGAEQEAGLRLAKLTDLLQQEEQGREVACGALQKNQ
  EDSSRRVDLEVARMQAQVTKLGEEVSLRFLKREAKLCGFLQKSFLALEKRMKASESSRLKLEGSLRGELE
  SRWEKLRGLMEERLRALQGQHEESHLLEQCQGLDAAVAQLTKFVQQNQASLNRVLLAEEKAWDAKGRLEE
  SRAGELAAYVQENLEAAQLAGELARQEMHGELVLLREKSRALEASVAQLAGQLKELSGHLPALSSRLDLQ
  EQMLGLRLSEAKTEWEGAERKSLEDLARWRKEVTEHLRGVREKVDGLPQQIESVSDKCLLHKSDSDLRIS
  AEGKAREFKVGALRQELATLLSSVQLLKEDNPGRKIAEMQGKLATFQNQIMKLENCVQANKTIQNLRFNT
  EARLRTQEMATLWESVLRLWSEEGPRTPLGSWKALPSLVRPRVFIKDMAPGKVVPMNCWGVYQAVRWLRW
  KASLIKLRALRRPGGVLEKPHSQEQVQQLTPSLFIQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001143980
• ORF Size: 2001 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001143980.1, NP_001137452.1
• RefSeq ORF: 2004
• Locus ID: 645811
• MW: 75.2 kDa