FAM157B (NM_001145249) Human Tagged ORF Clone

CAT#: RC227199

FAM157B (Myc-DDK-tagged)-Human family with sequence similarity 157, member B (FAM157B)

Product Images

Western blot validation of overexpression lysate (Cat# LY428760) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC227199 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FAM157B
• Synonyms: RP11-885N19.7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227199 representing NM_001145249
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCTTTGTTCACCACCATTCCTGGTGCCCATTCTGGTCCCATGCGTCCTCTGCCCAAGAAACACG
  TAGAACCCATGGCAGTGCGACAGCTGCTTCTTGGCAACTCCACCATGATCAGGCACACTTGTCCCATGTC
  TGTCCCGTTGAGCCGGCAAGTCAAGGAAGTTGCTGCCCAGAAACCAAGTGAGGACATTTACAAGAACCGG
  CAGCGGCGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACTGGATTTGCTTTTTCACCAGAGGA
  TCCAGATTTCCCTGTGGCCTCGCAAACAAAAAAGAAGGAAGACGGAGCAGCACAGTCATCCCTTTGTGAA
  AAAGGCATTCAGGTTCTCCGCCGGCCCTGGCTGCAGGCGGCCCTCATCTAACAAGATGCTTCGGTCCATG
  GGTGGAGGACAAAGGCCTATGGGCTTGGGGAGCGAGTTCTTTCGTCTCCTACATGACCTTCATCTGCTCG
  CGTTTGCCACGAAGCGCATCTGGATCCACCGGAGGGGCGAGGCCACAGCTCGACCGCGAGCACCTGAGCA
  TCCTGCACCGCCTGCGACGGCCGTCAGGGGGCGCGATGCCGCCTCACAGAACCTCAAGCGGCGCCCGGGT
  TCCGGCACAGACGGCCTGCGGCTCCAGGGGGCGGAGCCGAGCCGTCTCCTCAGGACCTACGCAGGAGGCG
  CCGTCATCCCTACAGGGACACCTGAGAGGGCCCAGCCTCCTCCTCCTCAGGACCCACTCGGGAGACGCCG
  CTGGCTCTCTAGGAACACCTGGGGACCGTGGCCAGGGACGACCCAGCCTCCTTCTCCTCAGCTCCTCAGG
  AACGACTGGGGAAGCTGTGGCTTCATGGTCCCCGAGGCTGCCCGAGGAAAGGTATTTCAGGACAGCCAGG
  AGGGGGCGCACATCCGCCGCGAAACTGTGAGCAAGAGCGTCTGTGCTGAACCATGGCGCCACCAGAGGGC
  GCGCGATCCCGCCCCAACCAACTTCCCGCTGAAGTGCCAGAAGCAGCGAGGAGCTTCAACTTCCTCAGGG
  CAGCACGGGGGTCGTGTTAATTTGGTGTTCTTCATTGATTCCCCAACAGTTATTGCTGTACCAGATTTGC
  AGTGTCCCACAAAATACTCCGGTATATTGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227199 representing NM_001145249
  Red=Cloning site Green=Tags(s)
  
  MGPLFTTIPGAHSGPMRPLPKKHVEPMAVRQLLLGNSTMIRHTCPMSVPLSRQVKEVAAQKPSEDIYKNR
  QRRQQQQQQQQQQQQLDLLFHQRIQISLWPRKQKRRKTEQHSHPFVKKAFRFSAGPGCRRPSSNKMLRSM
  GGGQRPMGLGSEFFRLLHDLHLLAFATKRIWIHRRGEATARPRAPEHPAPPATAVRGRDAASQNLKRRPG
  SGTDGLRLQGAEPSRLLRTYAGGAVIPTGTPERAQPPPPQDPLGRRRWLSRNTWGPWPGTTQPPSPQLLR
  NDWGSCGFMVPEAARGKVFQDSQEGAHIRRETVSKSVCAEPWRHQRARDPAPTNFPLKCQKQRGASTSSG
  QHGGRVNLVFFIDSPTVIAVPDLQCPTKYSGILY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145249
• ORF Size: 1152 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001145249.1, NP_001138721.1
• RefSeq ORF: 1155
• Locus ID: 100132403
• MW: 42.9 kDa