CPT1B (NM_001145134) Human Tagged ORF Clone

CAT#: RC227244

CPT1B (Myc-DDK-tagged)-Human carnitine palmitoyltransferase 1B (muscle) (CPT1B), nuclear gene encoding mitochondrial protein, transcript variant 5

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CPT1B
• Synonyms: CPT1-M; CPT1M; CPTI; CPTI-M; M-CPT1; MCCPT1; MCPT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC227244 representing NM_001145134
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAAGCTCACCAGGCCGTGGCCTTCCAGTTCACGGTGACCCCAGACGGGGTCGACTTCCGGCTCA
  GTCGGGAGGCCCTGAAACACGTCTACCTGTCTGGGATCAACTCCTGGAAGAAACGCCTGATCCGCATCAA
  GAATGGCATCCTCAGGGGCGTGTACCCTGGCAGCCCCACCAGCTGGCTGGTCGTCATCATGGCAACAGTG
  GGTTCCTCCTTCTGCAACGTGGACATCTCCTTGGGGCTGGTCAGTTGCATCCAGAGATGCCTCCCTCAGG
  GGTGTGGCCCCTACCAGACCCCGCAGACCCGGGCACTTCTCAGCATGGCCATCTTCTCCACGGGCGTCTG
  GGTGACGGGCATCTTCTTCTTCCGCCAAACCCTGAAGCTGCTTCTCTGCTACCATGGGTGGATGTTTGAG
  ATGCATGGCAAGACCAGCAACTTGACCAGGATCTGGGCTTACCTAGAGTCTGTGCGCCCCTTGTTGGATG
  ATGAGGAATATTACCGCATGGAGTTGCTGGCCAAAGAATTCCAGGACAAGACTGCCCCCAGGCTGCAGAA
  ATACCTGGTGCTCAAGTCATGGTGGGCAAGTAACTATGTGAGTGACTGGTGGGAAGAGTACATCTACCTT
  CGAGGCAGGAGCCCTCTCATGGTGAACAGCAACTATTATGTCATGGACCTTGTGCTCATCAAGAATACAG
  ACGTGCAGGCAGCCCGCCTGGGAAACATCATCCACGCCATGATCATGTATCGCCGTAAACTGGACCGTGA
  AGAAATCAAGCCTGTGATGGCACTGGGCATAGTGCCTATGTGCTCCTACCAGATGGAGAGGATGTTCAAC
  ACCACTCGGATCCCGGGCAAGGACACAGATGTGCTACAGCACCTCTCAGACAGCCGGCACGTGGCTGTCT
  ACCACAAGGGACGCTTCTTCAAGCTGTGGCTCTATGAGGGCGCCCGTCTGCTCAAGCCTCAGGATCTGGA
  GATGCAGTTCCAGAGGATCCTGGACGACCCCTCCCCACCTCAGCCTGGGGAGGAGAAGCTGGCAGCCCTC
  ACTGCAGGAGGAAGGGTGGAGTGGGCGCAGGCACGCCAGGCCTTCTTTAGCTCTGGAAAGAATAAGGCTG
  CCTTGGAGGCCATCGAGCGTGCCGCTTTCTTCGTGGCCCTGGATGAGGAATCCTACTCCTATGACCCCGA
  AGATGAGGCCAGCCTCAGCCTCTATGGCAAGGCCCTGCTACATGGCAACTGCTACAACAGGTGGTTTGAC
  AAATCCTTCACTCTCATTTCCTTCAAGAATGGCCAGTTGGGTCTCAATGCAGAGCATGCGTGGGCAGATG
  CTCCCATCATTGGGCACCTCTGGGAGTTTGTCCTGGGCACAGACAGCTTCCACCTGGGCTACACGGAGAC
  CGGGCACTGCCTGGGCAAACCGAACCCTGCGCTCGCACCTCCTACACGGCTGCAGTGGGACATTCCAAAA
  CAGTGCCAGGCGGTCATCGAGAGTTCCTACCAGGTGGCCAAGGCGTTGGCAGACGACGTGGAGTTGTACT
  GCTTCCAGTTCCTGCCCTTTGGCAAAGGCCTCATCAAGAAGTGCCGGACCAGCCCTGATGCCTTTGTGCA
  GATCGCGCTGCAGCTGGCTCACTTCCGGGACAGGGGTAAGTTCTGCCTGACCTATGAGGCCTCAATGACC
  AGAATGTTCCGGGAGGGACGGACTGAGACTGTGCGTTCCTGTACCAGCGAGTCCACAGCCTTTGTGCAGG
  CCATGATGGAGGGGTCCCACACAAAAGCAGACCTGCGAGATCTCTTCCAGAAGGCTGCTAAGAAGCACCA
  GAATATGTACCGCCTGGCCATGACCGGGGCAGGGATCGACAGGCACCTCTTCTGCCTTTACTTGGTCTCC
  AAGTACCTAGGAGTCAGCTCTCCTTTCCTTGCTGAGGTGCTCTCGGAACCCTGGCGTCTCTCCACCAGCC
  AGATCCCCCAATCCCAGATCCGCATGTTCGACCCAGAGCAGCACCCCAATCACCTGGGCGCTGGAGGTGG
  CTTTGGCCCTGTAGCAGATGATGGCTATGGAGTTTCCTACATGATTGCAGGCGAGAACACGATCTTCTTC
  CACATCTCCAGCAAGTTCTCAAGCTCAGAGACGAACGCCCAGCGCTTTGGAAACCACATCCGCAAAGCCC
  TGCTGGACATTGCTGATCTTTTCCAAGTTCCCAAGGCCTACAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC227244 representing NM_001145134
  Red=Cloning site Green=Tags(s)
  
  MAEAHQAVAFQFTVTPDGVDFRLSREALKHVYLSGINSWKKRLIRIKNGILRGVYPGSPTSWLVVIMATV
  GSSFCNVDISLGLVSCIQRCLPQGCGPYQTPQTRALLSMAIFSTGVWVTGIFFFRQTLKLLLCYHGWMFE
  MHGKTSNLTRIWAYLESVRPLLDDEEYYRMELLAKEFQDKTAPRLQKYLVLKSWWASNYVSDWWEEYIYL
  RGRSPLMVNSNYYVMDLVLIKNTDVQAARLGNIIHAMIMYRRKLDREEIKPVMALGIVPMCSYQMERMFN
  TTRIPGKDTDVLQHLSDSRHVAVYHKGRFFKLWLYEGARLLKPQDLEMQFQRILDDPSPPQPGEEKLAAL
  TAGGRVEWAQARQAFFSSGKNKAALEAIERAAFFVALDEESYSYDPEDEASLSLYGKALLHGNCYNRWFD
  KSFTLISFKNGQLGLNAEHAWADAPIIGHLWEFVLGTDSFHLGYTETGHCLGKPNPALAPPTRLQWDIPK
  QCQAVIESSYQVAKALADDVELYCFQFLPFGKGLIKKCRTSPDAFVQIALQLAHFRDRGKFCLTYEASMT
  RMFREGRTETVRSCTSESTAFVQAMMEGSHTKADLRDLFQKAAKKHQNMYRLAMTGAGIDRHLFCLYLVS
  KYLGVSSPFLAEVLSEPWRLSTSQIPQSQIRMFDPEQHPNHLGAGGGFGPVADDGYGVSYMIAGENTIFF
  HISSKFSSSETNAQRFGNHIRKALLDIADLFQVPKAYS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145134
• ORF Size: 2214 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145134.1, NP_001138606.1
• RefSeq ORF: 2217 bp
• Locus ID: 1375
• Cytogenetics: 22q13.33
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Fatty acid metabolism, PPAR signaling pathway
• MW: 83.7 kDa
• Gene Summary: 'The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene. [provided by RefSeq, Jun 2009]'