Synaptotagmin 1 (SYT1) (NM_001135806) Human Tagged ORF Clone
CAT#: RC227252
SYT1 (Myc-DDK-tagged)-Human synaptotagmin I (SYT1), transcript variant 3
"NM_001135806" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SYT1 |
Synonyms | BAGOS; P65; SVP65; SYT |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC227252 representing NM_001135806
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAGCGAGAGTCACCATGAGGCCCTGGCAGCCCCGCCTGTCACCACTGTCGCGACTGTTCTGCCAA GCAATGCCACAGAGCCAGCCAGTCCTGGAGAAGGAAAGGAAGATGCATTTTCTAAGCTGAAGGAGAAGTT TATGAATGAGTTGCATAAAATTCCATTGCCACCGTGGGCCTTAATTGCAATAGCCATAGTCGCAGTCCTT TTAGTCCTGACCTGCTGCTTTTGTATCTGTAAGAAATGTTTGTTCAAAAAGAAAAACAAGAAGAAGGGAA AGGAAAAAGGAGGGAAGAATGCCATTAACATGAAAGATGTAAAAGACTTAGGGAAGACGATGAAAGATCA GGCCCTCAAGGATGATGATGCTGAAACTGGATTGACAGATGGAGAAGAAAAAGAAGAACCCAAAGAAGAG GAGAAACTGGGAAAACTTCAGTATTCACTGGATTATGATTTCCAAAATAACCAGCTGCTGGTAGGGATCA TTCAGGCTGCCGAACTGCCCGCCTTGGACATGGGGGGCACATCTGATCCTTACGTGAAAGTGTTTCTGCT ACCTGATAAGAAGAAGAAATTTGAGACAAAAGTCCACCGAAAAACCCTTAATCCTGTCTTCAATGAGCAA TTTACTTTCAAGGTACCATACTCGGAATTGGGTGGCAAAACCCTAGTGATGGCTGTATATGATTTTGATC GTTTCTCTAAGCATGACATCATTGGAGAATTTAAAGTCCCTATGAACACAGTGGATTTTGGCCATGTAAC TGAGGAATGGCGTGACCTGCAAAGTGCTGAGAAGGAAGAGCAAGAGAAATTGGGTGATATCTGCTTCTCC CTTCGCTACGTACCTACTGCTGGTAAGCTGACTGTTGTCATTCTGGAGGCAAAGAACCTGAAGAAGATGG ATGTGGGTGGCTTATCCGATCCTTATGTGAAGATTCATCTGATGCAGAATGGTAAGAGGCTGAAGAAGAA AAAGACAACAATTAAAAAGAACACACTTAACCCCTACTACAATGAGTCATTCAGCTTTGAAGTACCTTTT GAACAAATCCAGAAAGTGCAGGTGGTGGTAACTGTTTTGGACTATGACAAGATTGGCAAGAACGATGCCA TCGGCAAAGTCTTTGTGGGCTACAACAGCACCGGCGCGGAGCTGCGACACTGGTCAGACATGCTGGCCAA CCCCAGGCGACCTATTGCCCAGTGGCACACCCTGCAGGTAGAGGAGGAAGTTGATGCCATGCTGGCCGTC AAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC227252 representing NM_001135806
Red=Cloning site Green=Tags(s) MVSESHHEALAAPPVTTVATVLPSNATEPASPGEGKEDAFSKLKEKFMNELHKIPLPPWALIAIAIVAVL LVLTCCFCICKKCLFKKKNKKKGKEKGGKNAINMKDVKDLGKTMKDQALKDDDAETGLTDGEEKEEPKEE EKLGKLQYSLDYDFQNNQLLVGIIQAAELPALDMGGTSDPYVKVFLLPDKKKKFETKVHRKTLNPVFNEQ FTFKVPYSELGGKTLVMAVYDFDRFSKHDIIGEFKVPMNTVDFGHVTEEWRDLQSAEKEEQEKLGDICFS LRYVPTAGKLTVVILEAKNLKKMDVGGLSDPYVKIHLMQNGKRLKKKKTTIKKNTLNPYYNESFSFEVPF EQIQKVQVVVTVLDYDKIGKNDAIGKVFVGYNSTGAELRHWSDMLANPRRPIAQWHTLQVEEEVDAMLAV KK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001135806 |
ORF Size | 1266 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001135806.1, NP_001129278.1 |
RefSeq ORF | 1269 bp |
Locus ID | 6857 |
Cytogenetics | 12q21.2 |
Protein Families | Secreted Protein, Transmembrane |
MW | 47.4 kDa |
Gene Summary | 'The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC325081 | SYT1 (untagged)-Human synaptotagmin I (SYT1), transcript variant 3 |
USD 760.00 |
|
RG227252 | SYT1 (GFP-tagged) - Human synaptotagmin I (SYT1), transcript variant 3 |
USD 460.00 |
|
RC227252L3 | Lenti ORF clone of Human synaptotagmin I (SYT1), transcript variant 3, Myc-DDK-tagged |
USD 620.00 |
|
RC227252L4 | Lenti ORF clone of Human synaptotagmin I (SYT1), transcript variant 3, mGFP tagged |
USD 620.00 |
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